关键词: 奶牛')">奶牛, 脂肪肝, 激素, PEPCK-C, HSL, 瘦蛋白, mRNA丰度

Abstract:

【Objective】 To understand the changes of metabolism, endocrine and mRNA expression of three genes in liver and adipose of fatty liver cows. 【Method】 Ten fatty liver cows and ten health control cows were chosen from the same dairy cow farm were used in the experiment. Fourteen blood parameters and liver fat content were detected in the tested cows. Meanwhile, mRNA expression of liver PEPCK-C was measured by a semi-quantitative RT-PCR, of adipose Lp and HSL by a real time RT-PCR in the tested cows. 【Result】 In fatty liver cows, the concentration of plasma glucose decreased very significantly (P＜0.01), plasma NEFA and BHBA increased very markedly (P＜0.01), the liver fat content (about 41.88%) and activity of serum AST increased very significantly (P＜0.01), the level of serum TBI, γ-GT and CHE increased significantly (P＜0.05), compared with the health control cows. In fatty liver cows, the concentration of plasma Ins and ratio of Ins to Gn all increased significantly (P＜0.05), mRNA expression level of liver PEPCK-C increased markedly (P＜0.05), and of adipose HSL also decreased significantly (P＜0.05), compared with the health control cows. mRNA expression level of adipose Lp , the concentration of plasma Lp and NPY all were lower markedly in fatty liver cows than that in health control cows(P＜0.05). 【Conclusion】 These results suggested that fatty liver cows have serious energy metabolism disorder, and marked liver disfunction. It might be an important mechanism of energy metabolic disorder for fatty liver cows to have a disturbance between Ins and Gn, Lp and NPY, and a decreased mRNA level of liver PEPCK-C, fat Lp and HSL.

Key words: dairy cows')">dairy cows, fatty liver, hormones, PEPCK-C, HSL, leptin, mRNA abundance