关键词: 节瓜（Benincasa hispida var. chieh-qua）, 分子遗传图谱, AFLP, RAPD, SRAP, QTL

Abstract:

【Objective】 The aims of this paper are to construct a molecular genetic linkage map and locate the first pistillate flower node traits of chieh-qua, which will provide a scientific basis for establishing molecular marker of breeding, female gene cloning and gene transfer. 【Method】 In this study, a population of F2 generation including 115 individual plants was derived from the cross between the homozygous weak female lines G4 and the homozygous gynoecious line K36, the AFLP, RAPD and SRAP markers were used in constructing a molecular genetic map and mapping QTL traits controlling the first pistillate flower bearing node. 【Result】 This molecular genetic linkage map included 13 linkage groups and contained 93 AFLP markers, 16 RAPD markers, 35 SRAP markers respectively. This map was 1 651.9 cM, and the average distance between two markers was 11.47 cM. Mapping of the quantitative trait loci of the first pistillate flower node was analyzed by software QTL Network 2.0, and 3 QTLs were found on the map, named as fn1, fn2, fn3. fnl located on linkage group1, fn2 and fn3 located on linkage group6. They accounted for 62.54%, 0.2%, 37.39% of the phenotypic variance, respectively. 【Conclusion】 A genetic linkage map was firstly constructed,and three QTLs was located which control first pistillate flower node traits of chieh-qua, thus providing a scientific basis for further cloning of the female-related gene and molecular marker-assisted selection breeding.

Key words: chieh-qua (Benincasa hispida var. chieh-qua), molecular genetic map, AFLP, RAPD, SRAP, quantitative trait loci (QTL)