关键词: 磷脂氢谷胱甘肽过氧化物酶, 荧光定量PCR, 组织分布, 睾丸, 附睾

Abstract:

【Objective】 The objectives of this study were to evaluate the expression pattern of PHGPx in various tissues and localization of PHGPx in gonad of goat. 【Method】 Total RNA was isolated from various tissues, and mRNA expression was detected by real-time fluorescent quantitative PCR. Cellular localization of PHGPx in testes and epididymidis was examined by immunohistochemistry, respectively. 【Result】 The quantitative real-time PCR results showed that the rank order of PHGPx mRNA expression was testes＞＞heart＞cerebrum＞epididymidis＞kidney＞liver＞lung＞spleen＞Longissimus dorsi (LD) muscle, and testes＞＞epididymidis tail (EpT)＞epididymidis head (EpH)＞epididymidis body (EpB). There was a positive expression of PHGPx in interstitial tissue and spermatogenic cells of caprine testes. The expression of PHGPx in smooth muscle fibers (SMF) of EpB showed a weak positive signal, and simple columnar epithelium (SCE) around the ductus epididymidis (DE) of EpT showed a strong positive products. 【Conclusion】 Goat PHGPx mRNA appeared to be ubiquitously expressed almost in all tissues, which plays an important biological function. The immunohistochemistry results display the differently expressed region evidently between testes and epididymidis. Furthermore, the mechanism of dramaticly expressed in gonad needs to be further researched.

Key words: phospholipid hydroperoxide glutathione peroxidase (PHGPx), fluorescent quantitative PCR, tissue distribution, testes, epididymidis