关键词: 小麦条锈病, 抗病基因, YrVir1, SSR, 遗传分析

Abstract: 【Objective】The wheat cultivar Virgilio is one of the wheat stripe rust differential hosts in China, it is very important to genetically characterize its stripe rust resistance genes. By developing the molecular markers linked to the resistance genes, we can detect the epidemics of stripe rust races and analyze wheat resistance on a molecular level. Meanwhile we map a stripe rust resistance gene YrVir1 in Virgilio using near-isogenic lines(NILs) Taichung29*6/YrVir1.【Method】We made a cross between cultivar Taichung29 and the resistance gene donor Virgilio, and developed the near-isogenic lines(NILs) Taichung29*6/YrVir1. The resistance of near-isogenic lineTaichung29*6/YrVir1, Taichung29 and their F2 progenies was tested by inoculating with stripe rust (Puccinia striiformis f. sp. tritici) single-spore isolate 2E16. We mapped the gene YrVir1 with 141 pairs SSR primers on wheat chromosome 2B.【Result】The resistance to 2E16 in Taichung29*6/YrVir1 was determined by a single dominant gene; a specific DNA band was amplified between Taichung29*6/YrVir1, Virgilio and Taichung29 by PCR with the primer Xbarc349. The genetic linkage was tested on 200 F2 plants, and the microsatellite marker Xbarc349 was linked to the resistance gene YrVir1, its genetic distance is 4.2cM.【Conclusion】Xbarc349 was regarded as SSR marker linked to stripe rust resistance gene YrVir1 in Chinese wheat differential host Virgilio. According to wheat SSR genetics map, the resistance gene YrVir1 was located on wheat chromosome 2B.

Key words: Wheat Stripe Rust, Resistance Gene, YrVir1, SSR, Genetic Analysis