关键词: 粗山羊草, 表达序列标签, 全长cDNA文库, 功能注释, 生物信息学

Abstract: Abstract: Expressed sequenced tags (EST) and full-length cDNA were important tool and resources for biological functional genomic and genetic research., the progenitor for D genome of bread wheat, was an important material for wheat genetic and genomic research.. Two full-length cDNA libraries were constructed from shoot and root of Aegilops tauschii Y2282 respectively by use of Cap-trapper method and 6616 ESTs and 6973 ESTs were obtained from the 2 libraries respectively. By use of our bioinformatic analysis platform base on Linux system ， we did electronic annotation for the EST, including blasting against public database such as protein ,nucleotide and triticum and Aegilops EST databases from NCBI. By use of result of GO ontology catalogue ,which was obtained from software GoPipee, and expression profile ,which were come from Phrap assembling result, some EST differently or specifically expressed in 2 cDNA libraries were obtained. Maybe the results could help us understand the molecular basis of different functions which organs perform.

Key words: Aegilops taushii, EST, Full-length cDNA library, Function annotation, Bioinformatics