关键词: 黑腹果蝇, 抗真菌肽, Drosomycin, 同系物, 表达, 蛋白纯化

Abstract: Drosomycin(Drs) is an insect antifungal peptide include 44-residue from Drosophila melanogaster only has broad-spectrum activity against filamentous fungi. Expect drs gene, there are six genes, Drs-lC, Drs-lD, Drs-lE, Drs-lF, Drs-lG and Drs-lH, showed homology to the Drs formed a gene family on the 3rd chromosome of D. melanogaster. Their mature peptides have the same guard sites and the CS徕 motif. In order to identify the function of these six members and study the relations of molecular structure and the function, these peptides should be cloned and expressed to obtain purified native sample. In this paper, six sequences were synthesized by two steps of PCR, cloned to the pET-3C vector and expressed in the E.coli origami, the expression products were purified by CM Sepharose?Fast Flow, Sephacryl S-100 High Resolution, Sephadex?G-25 medium and treated to retrieve the dimensional structure, finally the native peptides were obtained.