中国农业科学 ›› 2007, Vol. 40 ›› Issue (12): 2915-2919 .

• 研究简报 • 上一篇    下一篇

黄瓜CSHSP70基因内含子结构分析

杨寅桂,娄群峰,陈劲枫,刘 强,李为观   

  1. 作物遗传与种质创新国家重点实验室/南京农业大学园艺学院
  • 收稿日期:2007-01-25 修回日期:2007-03-09 出版日期:2007-12-10 发布日期:2007-12-10
  • 通讯作者: 陈劲枫

Structural Analysis of the Introns in Cucumber CSHSP70 Gene

  1. 作物遗传与种质创新国家重点实验室/南京农业大学园艺学院
  • Received:2007-01-25 Revised:2007-03-09 Online:2007-12-10 Published:2007-12-10

摘要: 【目的】探明黄瓜CSHSP70基因的内含子数目及其结构特点。【方法】根据黄瓜CSHSP70基因cDNA序列设计引物,应用PCR技术从gDNA中克隆CSHSP70基因,采用RT-PCR技术验证内含子的存在。【结果】经测序与拼接,得到长度为4 056 bp的黄瓜CSHSP70基因,它包含7个内含子。经分析发现这些内含子富含A•T,具有类似启动子和HSE核心结构的序列存在。对黄瓜、甜瓜和丝瓜CSHSP70基因部分序列的比较分析表明,该基因在这3个葫芦科作物中具有很强的保守性。【结论】内含子存在的特殊结构表明其可能对该基因表达具有调控作用。

关键词: 黄瓜, 叶绿体基质HSP70基因, 内含子

Abstract: 【Objective】Introns widely exist in the DNA region of non-encoding proteins and it could effectually regulate the gene expression in the eukaryote. The objective of this paper is to study the number and structure of the introns of the chloroplastic stroma HSP70 (CSHSP70) gene in the cucumber. 【Method】Primers were designed according to the cDNA sequence from cucumber CSHSP70 gene and cucumber CSHSP70 gene was cloned from gDNA with polymerase chain reaction (PCR) technique. The existence of introns was proven by reverse transcription polymerase chain reaction (RT-PCR) technique. 【Result】After sequencing the three fragments cloned from cucumber CSHSP70 gene, a 4056bp cucumber CSHSP70 gene with seven introns was obtained. Analysis of those introns indicated that they are rich in A•T, have similar structure of promoter and core structure of the HSE. Comparative analysis of the CSHSP70 sequence in cucumber, melon and luffa indicated its nature of conservative among those cucurbit crops. 【Result】The results obtained in this study are useful in revealing the introns function of the cucumber CSHSP70 gene and understanding the molecular mechanism of heat injury and heat tolerance.

Key words: Cucumis sativus L, CSHSP70 gene, intron