中国农业科学 ›› 2006, Vol. 39 ›› Issue (12): 2608-2614 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

利用RAPD标记分析柞蚕品种资源的亲缘关系

刘彦群,鲁成,秦利,向仲怀   

  1. 南开大学
  • 收稿日期:2005-07-06 修回日期:1900-01-01 出版日期:2006-12-10 发布日期:2006-12-10
  • 通讯作者: 鲁成

Genetic Relationships of Antheraea pernyi Cultivars based on RAPD Markers

YanQun Liu,Cheng Lu,Li Qin,ZhongHuai Xiang   

  1. 南开大学
  • Received:2005-07-06 Revised:1900-01-01 Online:2006-12-10 Published:2006-12-10
  • Contact: YanQun Liu

摘要: 【目的】从分子水平揭示柞蚕品种资源之间的遗传关系,为柞蚕的育种及种质资源的利用提供依据。【方法】利用RAPD(random amplified polymorphic DNA)技术,对来自中国6个主要柞蚕产区的66个品种(系)和2个朝鲜的品种的基因组DNA多态性进行分析。根据扩增结果计算单匹配相似系数,用Neighbor-Joining法构建聚类图。【结果】筛选出的33个随机引物对68个柞蚕品种(系)共扩增出296条DNA带,其中多态性带269条(90.88%)。品种间的成对遗传距离在0.120~0.324之间,主要集中在0.200~0.300之间,有99.05%的品种对的遗传距离小于0.300。聚类分析表明,供试的68个柞蚕品种(系)聚为6个类群,并表现出按目前所在地域(产地)聚类的特性。【结论】柞蚕品种资源之间的遗传差异较小,并且遗传聚类与其目前的地理分布有密切相关性。

关键词: 柞蚕, 品种, RAPD, 遗传关系

Abstract: The genetic relationships of 68 A. pernyi cultivars were analyzed with random amplified polymorphic DNA (RAPD). A total of 296 DNA fragments were amplified with 33 primers selected among A. pernyi cultivars, 269 of which (90.88%) were polymorphic. Only nine (12.68%) of A. pernyi cultivars used had one or more cultivar-specific markers. The value of pairwise genetic distance were from 0.120 to 0.324, and most of them were in the range of 0.200 to 0.300. We found 99.05% of the cultivar pairs with pairwise genetic distance less than 0.300 and 0.95% of cultivar pairs with pairwise genetic distance exceeding 0.300. Generally, there was great genetic similarity among A. pernyi cultivars. A dendrogram was constructed by Neighbor-Joining method. The A. pernyi cultvars used were classified into 6 genetic groups. Cultivargroupings were closely related to their geographic distribution in the present.

Key words: Antheraea pernyi, Cultivar, RAPD, Genetic relationship