中国农业科学 ›› 2006, Vol. 39 ›› Issue (11): 2384- .

• 研究简报 • 上一篇    下一篇

苦马豆素-BSA免疫山羊的血清相关酶评价

童德文,董 强,赵宝玉,刘文明,李 立,赵 俊   

  1. 西北农林科技大学动物科技学院
  • 收稿日期:2006-01-17 修回日期:1900-01-01 出版日期:2006-11-10 发布日期:2006-11-10
  • 通讯作者: 童德文

Analysis on Related Serum Enzyme of SW-BSA Immune Goats

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  1. 西北农林科技大学动物科技学院
  • Received:2006-01-17 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10

摘要: 【目的】通过评价SW-BSA免疫山羊的血清相关酶,探索SW-BSA免疫接种后对动物机体组织器官的保护作用。【方法】将24只山羊随机分为免疫对照组、免疫攻毒组和攻毒组,其中免疫对照组和免疫攻毒组接种SW-BSA,免疫攻毒组和攻毒组拌料饲喂10 g/kg BW/d甘肃棘豆草粉攻毒。【结果】血清GOT、GPT、LDH、AKP、BUN、AMA和抗体效价的检测结果表明,免疫攻毒组山羊较攻毒组山羊LDH活性升高延缓28 d,AKP活性升高延缓14 d,AMA活性降低延缓21 d,BUN活性升高延缓14 d,GOT活性升高延缓28 d。攻毒后免疫攻毒组山羊抗SW抗体水平降低,但在攻毒的第21天有一反弹,之后逐渐下降。【结论】这些酶活性延缓变化和抗体水平变化,说明在甘肃棘豆攻毒的前30 d内,SW-BSA能够有效地延缓SW对山羊肝脏、心脏和肾脏等组织器官的损伤。

关键词: SW-BSA, 血清酶, 甘肃棘豆, 山羊

Abstract: Abstract:24 goats were separated into immune control, immune and poisoning, poisoning groups randomly. Goats in immune control, immune and poisoning groups were vaccinated with SW-BSA. Goats in immune and poisoning, poisoning groups were fed with 10 g/kg BW/d power of O. kansuensis Bunge at each day. The changes of serum GOT, GPT, LDH, AKP, BUN and AMA were detected. The results showed that the increasing activity of LDH and GOT had been delayed 28 d, the increasing activity of AKP and BUN had been delayed 14 d, the decreasing activity of AMA had been delayed 21 d in immune and poisoning goats compared with poisoning goats. These results indicated that in 30 days, SW-BSA had prevented liver, kidney and heart of goats from injuring by SW effectively.