关键词: 野生稻, 种质资源, AFLP, DNA序列, RAPD

Abstract: 【Objective】A new cytoplamic male sterility line Yewei A and its maintainer line Yewei B, were developed from a maintainer line variant V20B with DNA transformed from wild rice(Oryza. Minuta J.S. Presl. ex C. B. Presl.). Experiment was carried out to verify if specific DNA fragment from O. minuta was integrated into genomes of the two species (YeweiB and YeweiA), and to verify if integrated fragment can inherit stably in high generation of YeweiB/YeweiA, 【Method】 Analysis of RAPD, DNA sequencing of specific RAPD bands and AFLP in receptor, variant and donor confirmed the integration and its stable inheritance. 【Result】Results of RAPD analysis of donor, receptor and variant gave that specific bands showed in variant and donor but not in receptor. DNA sequencing of the bands detected in RAPD suggested that primer OPG-11 could be used to amplify a 975 bp product in both variant and donor. A 97% similarity of the products was observed except a 29bp long difference. Mutated base pairs between the two DNA fragments included transition, transversion, insertion and deletion. At the same time, more polymorphisms was displayed in AFLP analysis between variants Yewei B and its receptor V20B with specific DNA fragments from O. minuta integrated. 【Conclusion】The above results confirmed that genomic DNA transformation from distant rice relatives was an effective approach to create new germplasm.