关键词: 麦长管蚜, 单克隆抗体, 酶联免疫吸附试验, 制备, 特性

Abstract: 【Objective】The English grain aphid, Sitobion avenae (Fabricius), is one of main wheat pests all over the world. It is very important to fully exert the natural control of predatory natural enemies in wheat fields in order to control the wheat aphid. 【Method】Hybridoma technolgy was used to develope monoclonal antibodies(McAbs) to the English grain aphid. The classes and subclasses of McAbs were determined by a double-antibody sandwich ELISA. Antibody specificity was tested using an indirect ELISA. SDS-PAGE and Western blot analysis were used to determine the proteins reacted with the antibodies.【Results】Two highly specific monoclonal antibodies were developed, namely EGA-1E8 and EGA-4H11, respectively. McAb EGA-1E8 was IgM subclass while McAb EGA-4H11 belonged to IgG2b subclass. McAb EGA-4H11 reacted with a single peptide of molecular weight 54.2 kD. The specificity test showed that both McAbs had no cross-reaction with other insects and spiders in wheat fields. 【Conclusion】The successful development of S. avenae McAbs may establish an essential tool for further study on the predatory natural enemies of the wheat aphid in wheat fields.

Key words: Macrosiphum avenae(Fabricius), Monoclonal antibody, ELISA, development, characterization