关键词: 小麦, 多枝赖草, 二体异附加系, 荧光原位杂交, 微卫星标记

Abstract: 【Objective】The salt-resistance and the alien addition chromosomes of the addition line Line15 were studied in an experiment. 【Method】The salt-resistance was detected under 0.4% ( NaCl/soil w/w) salt stress in man-made simulated salt pool. The numbers of chromosomes in the root cells and pollen cells was also checked. Then Line15 was detected by fluorescence in situ hybridization (FISH) and simple sequence repeat (SSR).【Result】The results showed that the wheat-Leymus multicaulis offspring Line15 was a salt-resistant line, and it was a disomic alien addition line which karyotype was 2n=44=22II. The results of SSR showed that the two alien addition chromosomes are closely correlated to the long arm of the second homologous group, the centromere region of the third homologous group and the short arm of the seventh homologous group in wheat, respectively. 【Conclusion】In this research, the wheat-Leymus multicaulis disomic alien addition line Line15 was detected on physiological, cellular and molecular level. The research results have provided reliable evidence for the source of the salt-resistance of Line15.

Key words: Triticum aestivum, Leymus multicaulis, Disomic alien addition line, FISH, SSR