中国农业科学 ›› 2005, Vol. 38 ›› Issue (09): 1796-1800 .

• 植物保护 • 上一篇    下一篇

小麦抗叶锈病基因Lr19的AFLP标记

李星,杨文香,刘大群,闫红飞,孟庆芳,张汀,李亚宁   

  1. 河北农业大学植物病理系
  • 收稿日期:2005-03-20 修回日期:1900-01-01 出版日期:2005-09-10 发布日期:2005-09-10
  • 通讯作者: 刘大群

Identification of AFLP Markers Linked to Lr19 Resistance to Wheat Leaf Rust

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  1. 河北农业大学植物病理系
  • Received:2005-03-20 Revised:1900-01-01 Online:2005-09-10 Published:2005-09-10

摘要: 以Thatcher和23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系及TcLr19与Thatcher杂交F2代植株为材料,利用AFLP技术开展了小麦抗叶锈病基因Lr19的分子标记研究。共获得7个与小麦抗叶锈病基因连锁的分子标记:P-AGT/M-GAG289bp(3.3cM)、P-ACA/M-GGT102bp(4.1cM)、P-ACA/M-GGT106bp(4.1cM)、P-AAC/M-CAG123bp(4.9cM)、P-AAC/M-GGT203bp(5.0cM)、P-ACA/M-GGT290bp(5.7cM)和P-ATC/M-GAG293bp(9.6cM)。这些特异性片段经回收、克隆、测序得出了特异带的序列。该研究可促进遗传图谱、物理图谱的构建和小麦抗叶锈病基因Lr19的克隆。

关键词: 小麦, Lr19, 分子标记, AFLP

Abstract: AFLP analysis was carried out on Thatcher, twenty three near?isogenic lines and F2 generation of Tclr19×Thatcher and developed molecular markers for gene Lr19 resistance to wheat leaf rust. Seven markers linked to Lr19 resistance traits were obtained, which were P-AGT/M-GAG289bp (3.3cM), P-ACA/M-GGT102bp (4.1cM), P-ACA/M-GGT106bp (4.1cM), P-AAC/M- CAG123bp (4.9cM), P-AAC/M-GGT203bp (5.0cM), P-ACA/M-GGT290bp (5.7cM), and P-ATC/M-GAG293bp (9.6cM). All of these specific fragments were isolated from the polyacrylamide gels, re-amplified, cloned and sequenced. The research may facilitate genetic mapping, physical mapping, and eventual cloning of Lr19 gene.

Key words: wheat, Lr19, molecular marker, AFLP