中国农业科学 ›› 2005, Vol. 38 ›› Issue (02): 405-409 .

• 畜牧.兽医.资源昆虫 • 上一篇    下一篇

IBDV超强毒株感染SPF鸡后法氏囊淋巴细胞凋亡及Bax和Bcl-2蛋白动态表达的研究

刘玉锋,佘锐萍   

  1. 中国农业大学动物医学院
  • 收稿日期:2004-02-17 修回日期:2004-10-26 出版日期:2005-02-10 发布日期:2005-02-10
  • 通讯作者: 刘玉锋

The Apoptosis and Dynamic Expression of Bax and Bcl-2 in Lymphocytes of Bursa of SPF Chickens Infected with SNJ93 Strain

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  1. 中国农业大学动物医学院
  • Received:2004-02-17 Revised:2004-10-26 Online:2005-02-10 Published:2005-02-10

摘要: 为证实传染性法氏囊炎超强毒SNJ93株感染SPF鸡后1~14 d内法氏囊淋巴细胞中Bcl-2、Bax蛋白的动态表达与淋巴细胞凋亡的关系。应用透射电镜观察、TUNEL法标记检测凋亡的淋巴细胞,用免疫组织化学方法检测Bcl-2、Bax蛋白。结果表明,感染后1~7 d可见到大量凋亡的淋巴细胞,其中感染后3~5 d最多。感染后1~9 d Bcl-2蛋白显著高于同时点的对照组(P<0.01);感染后1~5 d Bax蛋白明显高于同时点的对照组,差异极显著(P<0.01);感染后第7天高于同时点的对照组,差异显著(P<0.05); Bcl-2/Bax比率在感染后第1天低于同时点的对照组(P<0.05);3~5 d明显低于同时点的对照组(P<0.01)。SNJ93感染SPF雏鸡后1~7 d内法氏囊淋巴细胞会发生大量凋亡,Bcl-2/Bax能准确反映淋巴细胞的凋亡程度。

关键词: SPF鸡, 法氏囊, 细胞凋亡, Bax, Bcl-2

Abstract: Apoptosis and dynamic expression of their regulatory genes Bcl-2 and Bax in lymphocytes of bursa of 21-day -old SPF chickens infected with SNJ93 strain from the first to fourteenth day post infection were investigated. Apoptosis was observed by electron microscope and apoptosis rate was assessed by TUNEL method. The expression of Bcl-2, Bax was investigated by immunohistochemical ABC method. The apoptosis of lymphocytes in bursa of chickens infected with SNJ93 strain from the first to seventh day post infection significantly increased compared with that of normal SPF chickens (P<0.01).The expression of BCL-2 significantly increased in lymphocytes of bursa of SPF chickens infected with SNJ93 strain from the first to nineth day post infection compared with those of normal chickens (P<0.01). The expression of Bax significantly increased from the first to seventh day post infection (P<0.01 or P<0.05).The ratio of bcl-2 and Bax significantly decreased from the first to fifth day post infection (P<0.05 or P<0.01). A great deal of disappearance of lymphocytes of bursa of SPF chickens infected with SNJ93 strain from the first to seventh day was related to apoptosis .The ratio of Bcl-2 and Bax could precisely reflect the degree of apoptosis of lymphocytes of bursa.

Key words: SPF chickens, Bursa of Fabricius, Bpoptosis, Bcl-2, Bax