中国农业科学 ›› 2013, Vol. 46 ›› Issue (18): 3914-3921.doi: 10.3864/j.issn.0578-1752.2013.18.022

• 畜牧.兽医.资源昆虫 • 上一篇    下一篇

甘草酸二钾体外诱导感染MDV鸡胚成纤维细胞 凋亡发挥抗病毒作用

 丛向明1, 牛丽1, 白喜云1, 武彩红2, 孙耀贵1, 贺俊平1, 白元生3, 李宏全1   

  1. 1.山西农业大学动物科技学院,山西太谷 030801
    2.江苏畜牧兽医职业技术学院,江苏泰州 225300
    3.山西省生态畜牧产业管理站,太原 030001
  • 收稿日期:2012-12-30 出版日期:2013-09-15 发布日期:2013-06-28
  • 通讯作者: 通信作者李宏全,Tel:0354-6288409;E-mail:livets@163.com
  • 作者简介:丛向明,E-mail:congxiangming@sina.com
  • 基金资助:

    山西省科技攻关项目(2010311047,20120311022-1)

Dipotassium Glycyrrhizinate Against MDV on CEFs by Promoting MDV-Infected Cells Apoptosis in vitro

 CONG  Xiang-Ming-1, NIU  Li-1, BAI  Xi-Yun-1, WU  Cai-Hong-2, SUN  Yao-Gui-1, HE  Jun-Ping-1, BAI  Yuan-Sheng-3, LI  Hong-Quan-1   

  1. 1.College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi
    2.Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, Jiangsu
    3.Shanxi Province Station of Ecological Animal Husbandry     Industry Management, Taiyuan 030001
  • Received:2012-12-30 Online:2013-09-15 Published:2013-06-28

摘要: 【目的】探讨甘草酸二钾体外抗MDV的活性及作用机制。【方法】在体外以鸡胚成纤维细胞为MDV增殖模型,利用MTT比色法、噬斑减少法通过抗病毒试验、病毒复制抑制试验、直接灭活试验来评价甘草酸二钾的抗病毒效果;并通过细胞凋亡试验解释其抗病毒机制。【结果】抗病毒试验结果表明,在安全浓度范围内,甘草酸二钾在体外具有较强的抗MDV的活性,其EC50为(893.5±36.99)µg•mL-1,SI>3.36,最大抑制率达94.4%;在病毒复制抑制试验中,甘草酸二钾可以抑制病毒复制的整个过程;病毒直接灭活试验表明甘草酸二钾可直接杀灭病毒粒子且不呈时间依赖性;细胞凋亡试验结果显示甘草酸二钾能够诱导感染MDV的鸡胚成纤维细胞凋亡。【结论】甘草酸二钾在体外具有抗MDV的活性,可以通过诱导感染病毒的鸡胚成纤维细胞凋亡发挥抗病毒作用。

关键词: 甘草酸二钾 , MDV , 抗病毒 , 细胞凋亡

Abstract: 【Objective】The aim of the study is to test the antiviral activity and mechanism of dipotassium glycyrrhizinate (DG) against Marek’s disease virus (MDV) in vitro.【Method】 Using MTT test and standard plaque reduction assay on chick embryo fibroblast cells (CEFs), the antiviral effect of DG against MDV was observed by antiviral assay, time-of-addition assay and virucidal assay, and the antiviral mechanism was tested by cell apoptosis assay.【Result】Antiviral assay showed that DG could reduce the damage of MDV to CEFs , EC50 was (893.5±36.99) µg•mL-1,SI>3.36 and the inhibition ratio was 94.4%, at the maximum with no-cytotoxic concentration. Time-of-addition assay and virucidal assay demonstrated that DG could inhibit the viral replication in the full replication cycle and inactivated MDV particles in non time-dependent manner. Moreover, DG could promote MDV-infected cells apoptosis in vitro.【Conclusion】DG could inhibit MDV on CEFs by promoting MDV-infected cells apoptosis in vitro.

Key words: dipotassium glycyrrhizinate , MDV , anti-virus , cell apoptosis