关键词: 猪白介素, 基因家族, 同源性分析, 结构预测

Abstract: 【Objective】Aim This research is to clone, express and analyses the structure and function of major molecular of porcine interleukin family. 【Method】Genes of porcine interleukin family was cloned with RT-PCR through stimulated porcine PBMC by LPS and PHA firstly, then expressed in E.coli and predicted the structure and function of these molecular by ExPASY.【Result】It was successfully cloned and express the genes of IL-4, IL-6 and IL-18. Products of recombinant expression of IL-4 and IL-6 both have more biological activity. In analysis of these genes with NCBI/GenBank data, the homologies of the nucleotide acid sequence are 99.25%, 99.21% and 100% respectively and have large difference among other animal species. Results of prediction show that these molecular all contain many phosphorylation, glycosylation, protein kinase and signal transduction bonding sites in secondary structure, and all are a compact globularity protein in space configuration. This character of structure is the basis for its more biological functions. 【Conclusion】We have successfully cloned, expressed and analysed the structure and function of key molecular of porcine interleukin family.

Key words: Porcine interleukin, Gene family, Analysis of homologies, Prediction of construction