中国农业科学 ›› 2021, Vol. 54 ›› Issue (24): 5240-5250.doi: 10.3864/j.issn.0578-1752.2021.24.007
魏启航1,2(),冯瑶2,马倩倩2,李艳丽2,刘元望2,李兆君2,*(
),任艳芳1,*(
)
收稿日期:
2020-12-11
接受日期:
2021-03-22
出版日期:
2021-12-16
发布日期:
2021-12-28
通讯作者:
李兆君,任艳芳
作者简介:
魏启航,Tel:15996166142;E-mail: 基金资助:
WEI QiHang1,2(),FENG Yao2,MA QianQian2,LI YanLi2,LIU YuanWang2,LI ZhaoJun2,*(
),REN YanFang1,*(
)
Received:
2020-12-11
Accepted:
2021-03-22
Online:
2021-12-16
Published:
2021-12-28
Contact:
ZhaoJun LI,YanFang REN
摘要:
【目的】二次发酵是实现鸡粪等养殖废弃物功能价值提高的关键阶段,本研究旨在将前期筛选得到的真菌应用于鸡粪堆肥的二次发酵阶段,探讨其促进二次发酵的效果并对其进行鉴定,为今后鸡粪的高值化与资源化利用提供理论依据和支撑。【方法】以鸡粪一次发酵产物为原料,添加实验室前期筛选的2株真菌(分别为FCM1和FCM3)进行二次发酵试验。共设置3个处理,分别为对照CK:原料中加入1%(体积L∶干物质量kg)无菌液体培养基;F1:原料中加入1% FCM1菌液;F3:原料中加入1% FCM3菌液。在二次发酵的不同时期,分析测定堆体温度、pH、电导率(EC)、铵态氮、硝态氮、种子发芽指数以及腐殖酸等指标。【结果】与对照相比,添加真菌主要促进了二次发酵阶段快速升温和腐殖化过程快速进行。与未接菌处理相比,真菌FCM3使堆料提前8 d进入升温期,真菌FCM1效果不佳,且在一定程度上延后了鸡粪堆肥的二次发酵。F3处理高温阶段(>50℃)持续了13 d,堆料的GI比对照处理提前25 d达到80%,且腐殖化程度较高(PHA=74.58%,PHA:胡敏酸/总腐殖酸)。相关分析表明,GI与pH、PHA显著正相关,与EC显著负相关。经形态和ITS rDNA测序等方法鉴定,FCM3为链格孢菌。【结论】真菌FCM3能够在一定程度上促进鸡粪堆肥二次发酵的进行,提高堆料的腐殖化程度。
魏启航,冯瑶,马倩倩,李艳丽,刘元望,李兆君,任艳芳. 促进堆肥二次发酵真菌在堆肥中的应用效果[J]. 中国农业科学, 2021, 54(24): 5240-5250.
WEI QiHang,FENG Yao,MA QianQian,LI YanLi,LIU YuanWang,LI ZhaoJun,REN YanFang. Application Effect of Fungi Promoting Secondary Fermentation in Composting[J]. Scientia Agricultura Sinica, 2021, 54(24): 5240-5250.
[1] | 牛新胜, 巨晓棠. 我国有机肥料资源及利用. 植物营养与肥料学报, 2017, 23(6):1462-1479. |
NIU X S, JU X T. Organic fertilizer resources and utilization in China. Journal of Plant Nutrition and Fertilizer, 2017, 23(6):1462-1479. (in Chinese) | |
[2] | 中国物资再生协会. 《关于推进农业废弃物资源化利用试点的方案》解读. 中国资源综合利用, 2016, 34(10):15-16. |
China National Resources Recycling Association. Interpretation of the plan for promoting the pilot of agricultural waste recycling. China Resources Comprehensive Utilization, 2016, 34(10):15-16. (in Chinese) | |
[3] | BLUEMLING B, WANG F. An institutional approach to manure recycling: Conduit brokerage in Sichuan Province, China. Resources, Conservation & Recycling, 2018, 139:396-406. |
[4] |
DOMINGO J, ROVIRA J, VILAVERT L, NADAL M, FIGUERAS M J, SCHUHMACHER M. Health risks for the population living in the vicinity of an integrated waste management facility: Screening environmental pollutants. Science of the Total Environment, 2015, 518/519:363-370.
doi: 10.1016/j.scitotenv.2015.03.010 |
[5] | 中华人民共和国国务院办公厅. 关于加快推进畜禽养殖废弃物资源化利用的意见. 北京: 中华人民共和国国务院办公厅, 2020. |
General Office of the State Council of the People's Republic of China. Opinions on accelerating resource utilization of livestock and poultry waste. Beijing: General Office of the State Council of the People's Republic of China, 2020. (in Chinese) | |
[6] | CHAURASIA A, MEENA B R, TRIPATHI A N, PANDEY K K, RAI A B, SINGH B. Actinomycetes: an unexplored microorganisms for plant growth promotion and biocontrol in vegetable crops. World Journal of Microbiology and Biotechnology, 2018, 9(34):132. |
[7] | 曹文胜, 曹军, 王阳, 刘永德. 微生物接种应用于好氧堆肥的研究进展. 绿色科技, 2016(24):18-19, 21. |
CAO W S, CAO J, WANG Y, LIU Y D. Application of microbial inoculation in aerobic composting: A review. Journal of Green Science and Technology, 2016(24):18-19, 21. (in Chinese) | |
[8] | 田伟. 牛粪高温堆肥过程中的物质变化、微生物多样性以及腐熟度评价研究[D]. 南京: 南京农业大学, 2012. |
TIAN W. Study of substance change, microbial diversity and maturity assessment during thermophilic composting of dairy manure[D]. Nanjing: Nanjing Agricultural University, 2012. (in Chinese) | |
[9] |
LI C N, LI H Y, YAO T, SU M, RAN F, HAN B, LI J H, LAN X J, ZHANG Y C, YANG X M, GUN S B. Microbial inoculation influences bacterial community succession and physicochemical characteristics during pig manure composting with corn straw. Bioresource Technology, 2019, 289:121653.
doi: 10.1016/j.biortech.2019.121653 |
[10] |
WANG J Q, LIU Z P, XIA J S, CHEN Y P. Effect of microbial inoculation on physicochemical properties and bacterial community structure of citrus peel composting. Bioresource Technology, 2019, 291:121843.
doi: 10.1016/j.biortech.2019.121843 |
[11] |
XIE X Y, ZHAO Y, SUN Q H, WANG X Q, CUI H Y, ZHANG X, LI Y J, WEI Z M. A novel method for contributing to composting start-up at low temperature by inoculating cold-adapted microbial consortium. Bioresource Technology, 2017, 238:39-47.
doi: 10.1016/j.biortech.2017.04.036 |
[12] | 尹静, 刘悦秋, 于峰, 蔡建超, 刘天月. 一株木质素降解菌的筛选鉴定及其在堆肥中的应用. 中国土壤与肥料, 2019(3):179-185. |
YIN J, LIU Y Q, YU F, CAI J C, LIU T Y. Screening and identi6cation of a lignin-degrading bacterium and its application in composting. Soil and Fertilizer Sciences in China, 2019(3):179-185. (in Chinese) | |
[13] | 孙旭, 汝超杰, 苏良湖, 陈玉东, 赵克强, 张龙江, 蔡金傍. 3种秸秆腐熟剂微生物组成及其腐熟效果. 江苏农业科学, 2018, 46(3):212-215. |
SUN X, RU C J, SU L H, CHEN Y D, ZHAO K Q, ZHANG L J, CAI J B. Microbial composition and maturity effect of three straw decomposition agents. Jiangsu Agricultural Sciences, 2018, 46(3):212-215. (in Chinese) | |
[14] |
LIU J, XU X H, LI H T, XU Y. Effect of microbiological inocula on chemical and physical properties and microbial community of cow manure compost. Biomass and Bioenergy, 2011, 35(8):3433-3439.
doi: 10.1016/j.biombioe.2011.03.042 |
[15] |
RAI R, SUTHAR S. Composting of toxic weed Parthenium hysterophorus: Nutrient changes, the fate of faecal coliforms, and biopesticide property assessment. Bioresource Technology, 2020, 311:123523.
doi: 10.1016/j.biortech.2020.123523 |
[16] | 王桂珍, 李兆君, 张树清, 马晓彤, 梁永超. 碳氮比对鸡粪堆肥中土霉素降解和堆肥参数的影响. 中国农业科学, 2013, 46(7):1399-1407. |
WANG G Z, LI Z J, ZHANG S Q, MA X T, LIANG Y C. Influence of C/N ratio on degradation of oxytetracycline and composting parameters during chicken manure composting. Scientia Agricultura Sinica, 2013, 46(7):1399-1407. (in Chinese) | |
[17] |
LIU Y W, FENG Y, CHENG D M, XUE J M, WAKELIN S A, HU H Y, LI Z J. Gentamicin degradation and changes in fungal diversity and physicochemical properties during composting of gentamicin production residue. Bioresource Technology, 2017, 244(Part1):905-912.
doi: 10.1016/j.biortech.2017.08.057 |
[18] | 鲍士旦. 土壤农化分析. 北京: 中国农业出版社, 2000. |
BAO S D. Soil and Agricultural Chemistry Analysis. Beijing: China Agriculture Press, 2000. (in Chinese) | |
[19] | 李恕艳, 李吉进, 张邦喜, 李国学, 李扬阳, 李丹阳. 菌剂对鸡粪堆肥腐殖质含量品质的影响. 农业工程学报, 2016, 32(S2):268-274. |
LI S Y, LI J J, ZHANG B X, LI G X, LI Y Y, LI D Y. Influence of inoculants on content and quality of humus during chicken manure composting. Transactions of the Chinese Society of Agricultural Engineering, 2016, 32(S2):268-274. (in Chinese) | |
[20] |
WANG G Y, KONG Y L, LIU Y, LI D Y, ZHANG X H, YUAN J, LI G X. Evolution of phytotoxicity during the active phase of co-composting of chicken manure, tobacco powder and mushroom substrate. Waste Management, 2020, 114:25-32.
doi: 10.1016/j.wasman.2020.06.034 |
[21] | 尹瑞, 张鹤, 邱慧珍, 杨慧珍, 李孟婵, 张春红, 王友玲. 不同碳氮比牛粪玉米秸秆堆肥的碳素转化规律. 甘肃农业大学学报, 2019, 54(5):68-78. |
YIN R, ZHANG H, QIU H Z, YANG H Z, LI M C, ZHANG C H, WANG Y L. Study on carbon conversion of cow dung/corn straw composting with different C/N ratios. Journal of Gansu Agricultural University, 2019, 54(5):68-78. (in Chinese) | |
[22] |
LIU H J, HUANG Y, DUAN W D, QIAO C C, SHEN Q R, LI R. Microbial community composition turnover and function in the mesophilic phase predetermine chicken manure composting efficiency. Bioresource Technology, 2020, 313:123658.
doi: 10.1016/j.biortech.2020.123658 |
[23] | 王玉, 张晶, 曹云, 吴华山, 黄红英, 刘爱民. 极端嗜热功能菌筛选及其促进堆肥腐熟效果研究. 农业环境科学学报, 2020, 39(7):1633-1642. |
WANG Y, ZHANG J, CAO Y, WU H S, HUANG H Y, LIU A M. Screening of functional extreme thermophiles and their effects on improving the maturation of composting. Journal of Agro-Environment Science, 2020, 39(7):1633-1642. (in Chinese) | |
[24] | 王信, 蔡晓剑, 王亚艺, 徐仲阳, 高旭升, 李松龄. 牛粪高温好氧堆肥中发酵菌剂筛选研究. 青海大学学报, 2019, 37(5):20-25. |
WANG X, CAI X J, WANG Y Y, XU Z Y, GAO X S, LI S L. Screening of fermentation agents in cow manure during its aerobic composting. Journal of Qinghai University, 2019, 37(5):20-25. (in Chinese) | |
[25] | 戴美玲, 江涛, 谭美, 卢盛杰, 何伟豪, 张振宇, 向铁军. 微生物菌剂对菜粕堆肥的影响及堆肥腐熟度评价. 湖南农业科学, 2020(2):29-32, 36. |
DAI M L, JIANG T, TAN M, LU S J, HE W H, ZHANG Z Y, XIANG T J. Effect of microbial agents on rapeseed meal composting and evaluation of compost maturity. Hunan Agricultural Sciences, 2020(2):29-32, 36. (in Chinese) | |
[26] | 高芬, 吴元华. 链格孢属(Alternaria)真菌病害的生物防治研究进展. 植物保护, 2008(3):1-6. |
GAO F, WU Y H. Progresses in the biocontrol of plant diseases caused by Alternaria. Plant Protection, 2008(3):1-6. (in Chinese) | |
[27] | 李朋华. 部分链格孢属真菌的形态学及多基因鉴定. 河南农业大学, 2016. |
LI P H. Identifiation of partial Alternaria by morphology and multiple-gene analysis. Henan Agricultural University, 2016. (in Chinese) | |
[28] | 李宏光, 易图永, 肖艳松, 巢进, 方明. 7%井冈霉素·极细链格孢激活蛋白可湿性粉剂对烟草病毒病的防效研究. 现代农业科技, 2014(24): 126, 131. |
LI H G, YI T Y, XIAO Y S, CHAO J, FANG M. Study on the Control Effect of 7 % Jinggangmycin·Alternaria activator protein wettable powder on tobacco virus disease. Modern Agricultural Science and Technology, 2014(24): 126, 131. (in Chinese) | |
[29] | YANG F Z, YANG B, LI B B, XIAO C. Alternaria toxin-induced resistance in rose plants against rose aphid (Macrosiphum rosivorum): effect of tenuaonic acid. Journal of Zhejiang University-SCIENCE B(Biomedicine & Biotechnology), 2015, 16(4):264-274. |
[30] |
XU Y L, LI X Y, CONG C, GONG G L, XU Y P, CHE J, HOU F Q, CHEN H L, WANG L L. Use of resistant Rhizoctonia cerealis strains to control wheat sharp eyespot using organically developed pig manure fertilizer. Science of the Total Environment, 2020, 726:138568.
doi: 10.1016/j.scitotenv.2020.138568 |
[31] |
LI H H, ZHANG T, TSANG D C W, LI G X. Effects of external additives: Biochar, bentonite, phosphate, on co-composting for swine manure and corn straw. Chemosphere, 2020, 248:125927.
doi: 10.1016/j.chemosphere.2020.125927 |
[32] |
AWASTHI M K, DUAN Y M, AWASTHI S K, LIU T, ZHANG Z Q, KIM S H, PANDEY A. Effect of biochar on emission, maturity and bacterial dynamics during sheep manure compositing. Renewable Energy, 2020, 152:421-429.
doi: 10.1016/j.renene.2020.01.065 |
[33] |
AWASTHI M K, PANDEY A K, BUNDELA P S, WONG J W C, LI R H, ZHANG Z Q. Co-composting of gelatin industry sludge combined with organic fraction of municipal solid waste and poultry waste employing zeolite mixed with enriched nitrifying bacterial consortium. Bioresource Technology, 2016, 213:181-189.
doi: 10.1016/j.biortech.2016.02.026 |
[34] |
HUANG G F, WONG J W C, WU Q T, NAGAR B B. Effect of C/N on composting of pig manure with sawdust. Waste Management, 2004, 24:805-813.
doi: 10.1016/j.wasman.2004.03.011 |
[35] |
PAN J T, LI R H, ZHANG Z Q, MA J Y, LIU H B. Influence of palygorskite addition on biosolids composting process enhancement. Journal of Cleaner Production, 2019, 217:371-379.
doi: 10.1016/j.jclepro.2019.01.227 |
[36] |
LIU T, AWASTHI M K, AWASTHI S K, REN X N, LIU X Y, ZHANG Z Q. Influence of fine coal gasification slag on greenhouse gases emission and volatile fatty acids during pig manure composting. Bioresource Technology, 2020, 316:123915.
doi: 10.1016/j.biortech.2020.123915 |
[37] | 魏启航, 任艳芳, 何俊瑜, 李兆君. 畜禽养殖废弃物堆肥过程中微生物除臭研究进展. 中国农业科学, 2020, 53(15):3134-3145. |
WEI Q H, REN Y F, HE J Y, LI Z J. Research progress of microbial deodorization in livestock and poultry wastes composting. Scientia Agricultura Sinica, 2020, 53(15):3134-3145. (in Chinese) | |
[38] |
BERNAL M P, PAREDES C, SÁNCHEZ-MONEDERO M A, CEGARRA J. Maturity and stability parameters of composts prepared with a wide range of organic wastes. Bioresource Technology, 1998, 63(1):91-99.
doi: 10.1016/S0960-8524(97)00084-9 |
[39] |
WANG G Y, KONG Y L, LIU Y, LI D Y, ZHANG X H, YUAN J, LI G X. Evolution of phytotoxicity during the active phase of co-composting of chicken manure, tobacco powder and mushroom substrate. Waste Management, 2020, 114:25-32.
doi: 10.1016/j.wasman.2020.06.034 |
[40] |
ESTEBAN Q, ARIZ I, CRUZ C, MORAN J F. Review Mechanisms of ammonium toxicity and the quest for tolerance. Plant Science, 2016, 248:92-101.
doi: 10.1016/j.plantsci.2016.04.008 |
[41] | ZHANG B X, XU Z C, JIANG T, HUDA N, LI G X, LUO W H. Gaseous emission and maturity in composting of livestock manure and tobacco wastes: Effects of aeration intensities and mitigation by physiochemical additives. Environment Technology & Innovation, 2020, 19:100899. |
[42] |
ZHAO X L, LI B Q, NI J P, XIE D T. Effect of four crop straws on transformation of organic matter during sewage sludge composting. Journal of Integrative Agriculture, 2016, 15(1):232-240.
doi: 10.1016/S2095-3119(14)60954-0 |
[43] | 许修宏, 马怀良. 接种菌剂对鸡粪堆肥腐殖酸的影响. 中国土壤与肥料, 2010(1):54-56. |
XU X H, MA H L. Influence of microbial inoculants on humic acids in compost of chicken manure. Soil and Fertilizer Sciences in China, 2010(1):54-56. (in Chinese) | |
[44] | 陈迪, 赵洪颜, 葛长明, 李金雪, 李雪, 朴仁哲. 中药渣堆肥化过程中腐殖酸的动态变化研究. 延边大学农学学报, 2015, 37(4):292-295. |
CHEN D, ZHAO H Y, GE C M, LI J X, LI X, PIAO R Z. Dynamics of humic acid during composting of Chinese Herbal Medicine. Journal of Agricultural Science Yanbian University, 2015, 37(4):292-295. (in Chinese) | |
[45] |
SHAN Y N, CHEN J H, WANG L, LI F, FU X H, LE Y Q. Influences of adding easily degradable organic waste on the minimization and humification of organic matter during straw composting. Journal of Environmental Science and Health, 2013, 48(5):384-392.
doi: 10.1080/03601234.2013.742391 |
[1] | 杨虹,曹文明,陈何妍,卫学青,束莉丹,李彤. 谷物及制品中修饰型真菌毒素的风险与防控[J]. 中国农业科学, 2022, 55(6): 1213-1226. |
[2] | 沙月霞, 黄泽阳, 马瑞. 嗜碱假单胞菌Ej2对稻瘟病的防治效果及对水稻内源激素的影响[J]. 中国农业科学, 2022, 55(2): 320-328. |
[3] | 张晨曦, 田明慧, 杨硕, 杜嘉琪, 何堂庆, 仇云鹏, 张学林. 酸性土壤中丛枝菌根真菌菌剂多样性对玉米产量及其磷钾吸收的影响[J]. 中国农业科学, 2022, 55(15): 2899-2910. |
[4] | 刘泓,郭玉杰,许雄,李侠,张鸿儒,齐立伟,孙雪梅,张春晖. 不同畜禽骨蛋白肽的制备、理化特性表征及其生物活性[J]. 中国农业科学, 2022, 55(13): 2629-2642. |
[5] | 张学林,何堂庆,张晨曦,田明慧,李晓立,吴梅,周亚男,郝晓峰. 丛枝菌根真菌对玉米生育期土壤N2O排放的影响[J]. 中国农业科学, 2022, 55(10): 2000-2012. |
[6] | 赵卫松,郭庆港,苏振贺,王培培,董丽红,胡卿,鹿秀云,张晓云,李社增,马平. 马铃薯健株与黄萎病株根际土壤真菌群落结构及其对碳源利用特征[J]. 中国农业科学, 2021, 54(2): 296-309. |
[7] | 范楷,祭芳,徐剑宏,钱鸣蓉,段劲生,聂冬霞,唐占敏,赵志辉,史建荣,韩铮. 长三角地区市场常见农产品中40种真菌毒素的污染状况和特征分析[J]. 中国农业科学, 2021, 54(13): 2870-2884. |
[8] | 孔亚丽,朱春权,曹小闯,朱练峰,金千瑜,洪小智,张均华. 土壤微生物介导植物抗盐性机理的研究进展[J]. 中国农业科学, 2021, 54(10): 2073-2083. |
[9] | 毕秋艳,党志红,朱伟旗,高占林,韩秀英,赵建江,王文桥,路粉,吴杰. 河北省大豆主要病原真菌鉴定及防治药剂筛选[J]. 中国农业科学, 2021, 54(1): 71-85. |
[10] | 田晴,高丹美,李慧,刘守伟,周新刚,吴凤芝. 小麦根系分泌物对西瓜连作土壤真菌群落结构的影响[J]. 中国农业科学, 2020, 53(5): 1018-1028. |
[11] | 唐科志,周常勇. 红橘响应褐斑病菌侵染的转录组学分析[J]. 中国农业科学, 2020, 53(22): 4584-4600. |
[12] | 付兵,王美,刘建阳,林伟,张成省,赵栋霖. 海洋来源杂色曲霉次级代谢产物及其抗植物病原细菌活性[J]. 中国农业科学, 2020, 53(19): 3964-3974. |
[13] | 赵艳,王天圻,朱军莉. 基于PTN系统分析不同种植地转基因水稻种子 可培养内生真菌菌群的多样性[J]. 中国农业科学, 2020, 53(11): 2305-2320. |
[14] | 刘海洋, 王伟, 张仁福, 热西达·阿不都热合曼, 姚举. 黄萎病不同发生程度棉田土壤中的真菌群落特征分析[J]. 中国农业科学, 2019, 52(3): 455-465. |
[15] | 郝宝成, 宋向东, 高艳, 王学红, 刘宇, 李元曦, 梁妍, 陈柯源, 胡毓瑶, 邢小勇, 胡永浩, 梁剑平. 产苦马豆素疯草内生真菌Alternaria Section Undifilum oxytropis的诱变筛选[J]. 中国农业科学, 2019, 52(15): 2716-2728. |
|