小麦TaBG的克隆及其在花药开裂中的潜在功能

doi:10.3864/j.issn.0578-1752.2021.13.002

中国农业科学 ›› 2021, Vol. 54 ›› Issue (13): 2710-2723.doi: 10.3864/j.issn.0578-1752.2021.13.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

小麦TaBG的克隆及其在花药开裂中的潜在功能

谭照国^1,²(),李艳梅²(),白建芳²,郭昊宇²,栗婷婷²,段文静²,刘子涵²,苑少华²,张天豹²,张风廷²,陈兆波²,赵福永¹(),赵昌平²(),张立平^1,²()

¹长江大学生命科学学院,湖北荆州 434025
²北京市农林科学院北京杂交小麦工程技术研究中心/杂交小麦分子遗传北京市重点实验室,北京 100097

收稿日期:2020-11-25 修回日期:2021-02-05 出版日期:2021-07-01 发布日期:2021-07-12
通讯作者: 赵福永,赵昌平,张立平
作者简介:谭照国,Tel：18977388904;E-mail： tanzhaoguo@foxmail.com。|李艳梅,E-mail： lymei1013@163.com。
基金资助:
国家自然科学基金(31872881);国家自然科学青年基金(31901490);北京市农林科学院杰出科学家项目(JKZX201907)

Cloning of TaBG and Analysis of Its Function in Anther Dehiscence in Wheat

TAN ZhaoGuo^1,²(),LI YanMei²(),BAI JianFang²,GUO HaoYu²,LI TingTing²,DUAN WenJing²,LIU ZiHan²,YUAN ShaoHua²,ZHANG TianBao²,ZHANG FengTing²,CHEN ZhaoBo²,ZHAO FuYong¹(),ZHAO ChangPing²(),ZHANG LiPing^1,²()

¹College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei
²Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences/The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097

Received:2020-11-25 Revised:2021-02-05 Online:2021-07-01 Published:2021-07-12
Contact: FuYong ZHAO,ChangPing ZHAO,LiPing ZHANG

摘要/Abstract

摘要：

【目的】β-葡萄糖苷酶（4-β-D-glueosidase,BG）是一种水解酶,可以从糖聚合物或寡聚糖中水解糖苷键释放出非还原性糖基,对控制花药开裂具有重要作用。小麦光温敏雄性不育系BS366在不育环境下花药不开裂,在可育环境下花药开裂或不完全开裂。从BS366中克隆TaBG,分析其在花药开裂中的潜在功能,为进一步解析小麦光温敏雄性不育系花药开裂异常的分子机理提供理论基础。【方法】以不育系BS366花药的cDNA为模板,克隆TaBG;利用生物信息学软件对TaBG及其蛋白结构进行预测,构建系统进化树、预测互作蛋白并对TaBG进行上游启动子元件以及互作miRNA预测;构建TaBG-16318hGFP载体,观察其在小麦原生质体中的亚细胞定位;使用实时荧光定量PCR（qPCR）方法测定TaBG在不育环境、可育环境下不同发育时期花药中的表达量,以及MeJA处理下TaBG及与其互作的tae-miR395a在花药和颖壳中的表达模式。【结果】TaBG属于糖基水解酶超家族基因,全长为1 473 bp,编码490个氨基酸,蛋白理论等电点为8.12,属于不稳定的亲水性蛋白。通过miRNA互作预测,发现TaBG可能受miR169、miR395a等抗逆性相关的miRNA调控。通过蛋白互作预测,发现TaBG可能与氧化还原酶（glucose-methanol-choline oxidoreductase,GMC）、内切葡聚糖酶（endoglucanase,EG）等蛋白互作。TaBG定位于小麦原生质体的液泡中。qPCR结果表明,TaBG在花药发育双室期（stage13)、花药开裂时期（stage14）和衰退期（stage15）中的表达量呈现先升高后下降的趋势。在花药开裂时期表达量最高,并且在不开裂花药中的表达量是正常开裂花药的2.8倍。经MeJA处理后,花药和颖壳中的TaBG均呈下调表达,tae-miR395a表达模式与其相反。【结论】TaBG可能受miR169、miR395a等抗逆相关miRNA的调控,参与花药开裂调控。由于TaBG表达量的升高,增加了不育环境下花药中可溶性糖的含量,进而增加花药中的渗透势,从而减缓花药开裂时的脱水活动,导致花药不开裂。

关键词: 小麦, β-葡萄糖苷酶, miRNA, 花药开裂

Abstract:

【Objective】 β-glucosidase (BG, 4-β-D-glueosidase) is a kind of hydrolase which hydrolyzes glycosidic bond from glycopolymer or oligosaccharide to release non reducing sugar group, which plays an important role in controlling anther dehiscence. The anthers of the photoperiod-temperature sensitive genic male sterile (PTGMS) wheat line BS366 did not dehiscence in sterile environment, but completely or partially cracked in fertile environment. In order to study the regulatory function of BG gene on anther dehiscence in wheat, the TaBG was cloned from BS366 and its potential function in anther dehiscence was analyzed, which provided theoretical basis for further analysis of molecular mechanism of abnormal anther dehiscence in photoperiod thermo sensitive male sterile line of wheat.【Method】TaBG was cloned from the anther of PTGMS line BS366, and the primary structure, secondary structure, tertiary structure of TaBG protein were predicted. In addition, the cis-acting elements in promoter region prediction, phylogenetic tree construction with other species, interaction between miRNA and TaBG were performed using bioinformatics software. The expression levels of TaBG and the interacted tae-miR395a in anthers and glumes under MeJA and SA treatment were determined. 【Result】 The total length of TaBG is 1 473 bp, which encoded 490 amino acids, and the theoretical pI is 8.12, belonging to the glycosylhydrolase superfamily. The results showed that TaBG may be regulated by stress resistance-related miRNAs, such as miR169and miR395a. Based on the prediction of protein interaction, it was found that TaBG might interact with glucose-methanol-choline oxidoreductase (GMC) and endoglucanase (EG). TaBG is located in the liquid bubble of the wheat proton. The qPCR results showed that the expression of TaBG in Bilocular stage (stage 13), Dehiscence (stage14) and Senescence (stage15) showed an upward and then downward trend. TaBG was the highest expression in Dehiscence, and the expression of TaBG in no dehiscence of anther was 2.8 times higher than that of complete dehiscence of anther. After treatment of MeJA, the expression ofTaBG in anther and glume showed downward. The tae-miR395a expression pattern was the opposite.【Conclusion】 TaBGmight be involved in anther dehiscence with stress tolerance related miRNA such as miR169andmiR395a. High expression of TaBG could increase the content of soluble sugar in the anther under the sterile environment, and then increase the osmotic potential in the anther, so as to slow down the dehydration of anther dehiscence. This study will lay a foundation for elucidating the biological function of BG in regulating anther dehiscence.

Key words: wheat, 4-β-D-glueosidase, miRNA, anther dehiscence

谭照国,李艳梅,白建芳,郭昊宇,栗婷婷,段文静,刘子涵,苑少华,张天豹,张风廷,陈兆波,赵福永,赵昌平,张立平. 小麦TaBG的克隆及其在花药开裂中的潜在功能[J]. 中国农业科学, 2021, 54(13): 2710-2723.

TAN ZhaoGuo,LI YanMei,BAI JianFang,GUO HaoYu,LI TingTing,DUAN WenJing,LIU ZiHan,YUAN ShaoHua,ZHANG TianBao,ZHANG FengTing,CHEN ZhaoBo,ZHAO FuYong,ZHAO ChangPing,ZHANG LiPing. Cloning of TaBG and Analysis of Its Function in Anther Dehiscence in Wheat[J]. Scientia Agricultura Sinica, 2021, 54(13): 2710-2723.

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引物名称 Primer name	引物序列 Primer sequence (5'-3')	用途 Usage
TaBG-F	CTCCACTATGGAGCTCCTCCG	基因扩增Gene amplification
TaBG-R	TATTCACCCATCTGGCACCACT	基因扩增Gene amplification
TaBG-CF	ATGGAGCTCCTCCGCG	蛋白编码区克隆 CDS cloning
TaBG-CR	TCAGTACTCCTCGCTCTT	蛋白编码区克隆 CDS cloning
TaBG-DF	TATCTCTAGAGGATCCATGGAGCTCCTCCGCG	亚细胞定位 Subcellular localization
TaBG-DR	TGCTCACCATGGATCCGTACTCCTCGCTCTTGATCAC	亚细胞定位 Subcellular localization
TaBG-QF	CCTCACGCGCTCTACGACACC	实时荧光定量PCR qPCR
TaBG-QR	CCATGCGAAGTAGCCCGTCACC	实时荧光定量PCR qPCR
β-Actin-QF	TACTCCCTCACAACAACCG	内参基因 Reference gene
β-Actin-QR	AGAACCTCCACTGAGAACAA	内参基因 Reference gene
tae-miR395a-QF	GTGAAGTGTTTGGGGGAACTC	实时荧光定量PCR qPCR
tae-miR395a-QR	AGAACTTCACAGTGGTCTTA	实时荧光定量PCR qPCR
U6snRNA-QF	CAAGGATGACACGCAAATTCG	内参基因 Reference gene
U6snRNA-QR	GTGCAGGGTCCGAGGT	内参基因 Reference gene

上游启动子元件 Upstream promoter elements	功能 Function
CAAT-box、TATA-box	核心启动子元件 Core promoter element
ABRE	脱落酸响应元件 Abscisic acid responsiveness element
CGTCA-motif、TGACG-motif	茉莉酸甲酯响应元件 MeJA-responsiveness responsiveness element
TCA-element	水杨酸响应元件 Salicylic acid responsiveness element
G-box、TCT-motif	光响应元件 Light responsiveness element
CAT-box	分生组织表达相关元件 Related to meristem expression element

基序 Motif	基序序列 Motif sequence
Motif 1	DFCFKTYGDRVKNWFTINEPRMMAWHGYGDGFFPPGRCTGC
Motif 2	CIWDTFLKYPGATPDNATANVTVDEYHRYMDDVDNMVRVGFDAYRFSISW
Motif 3	DGARVTGYFAWSLLDNFEWRLGFTSKFGJVYVDRKTFTRYPKDSTRWFRK
Motif 4	GKIGILLDFVWYEPLTYSVEDEYAAHRAREFTLGWFLHPITYGHYPETMZ
Motif 5	SRIFPSGIGRINKDGVDYYHRLIBYMLANNITPYVVLYHYDLPZVLNBQY
Motif 6	ERNGVPIGKQAYSDWLYVVPWGFYKAVMHVKEKFNBPVILIGENGIDQSG
Motif 7	KJVGGRLPNFTFEQSKMVKGSADYIAINHYTTYYVSHHVNLTHMSYANDW
Motif 8	LLANGEHTBLTRDSFPPGFVFGTASSAYQVEGNALKYGRGP
Motif 9	NSATEPYIAGHHLLLAHAAAVKVYRDKYQ
Motif 10	NDTLPHALYDTFRIDYFDQYLHELKRAIA

小麦TaBG的克隆及其在花药开裂中的潜在功能

Cloning of TaBG and Analysis of Its Function in Anther Dehiscence in Wheat

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