2017-2019年四川地区猪A群轮状病毒的分子流行病学调查

doi:10.3864/j.issn.0578-1752.2021.05.017

中国农业科学 ›› 2021, Vol. 54 ›› Issue (5): 1063-1072.doi: 10.3864/j.issn.0578-1752.2021.05.017

• 畜牧·兽医·资源昆虫 • 上一篇下一篇

2017-2019年四川地区猪A群轮状病毒的分子流行病学调查

周群¹(),陈小飞²(),阚蕊慈¹,李玉¹,曹慧¹,彭艳伶³,张斌^1,⁴()

¹西南民族大学生命科学与技术学院,成都 610041
²华南农业大学兽医学院,广州 510642
³西昌市畜牧局,四川西昌 615000
⁴青藏高原动物遗传资源保护与利用教育部/四川省重点实验室,成都 610041

收稿日期:2020-04-13 接受日期:2020-07-29 出版日期:2021-03-01 发布日期:2021-03-09
通讯作者: 张斌
作者简介:周群,E-mail：2297248747@qq.com。|陈小飞,E-mail：chenxf-1@163.com。
基金资助:
国家自然科学基金(31772766);四川应用基础研究计划(2020YJ0247);西南民族大学中央高校基本科研业务费专项资金(2020NYB21)

Molecular Epidemiological Investigation of Porcine Group A Rotavirus in Sichuan from 2017 to 2019

Qun ZHOU¹(),XiaoFei CHEN²(),RuiCi KAN¹,Yu LI¹,Hui CAO¹,YanLing PENG³,Bin ZHANG^1,⁴()

¹College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041
²College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642
³Animal Husbandry Bureau of Xichang City, Xichang 615000, Sichuan
⁴Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education/Sichuan Province, Chengdu 610041

Received:2020-04-13 Accepted:2020-07-29 Online:2021-03-01 Published:2021-03-09
Contact: Bin ZHANG

摘要/Abstract

摘要：

【目的】通过对四川地区规模化猪场A群轮状病毒（RVA）的分子检测,从而了解RVA流行情况及分子特征,为猪RVA疫苗研制提供理论基础。【方法】2017—2019年从四川省14个地区40个猪场采集303份仔猪腹泻样本,用荧光定量RT-PCR方法调查RVA的分子流行率,用RT-PCR方法对RVA阳性样本进行分型和VP4和VP7全基因组的扩增,用RotaC^2.0软件确定相应毒株的基因型,用MegAlign软件进行同源性分析,通过MEGA 7.0软件用邻近法构建系统进化树,用SimPlot和RDP4软件进行重组分析。【结果】303份仔猪腹泻样本检出RVA阳性样本98份,阳性率为32.34%（98/303,95%CI=27.1%—37.9%）。从98份RVA阳性样本中扩增出39个VP7片段,G9为优势基因型（41%）,G4、G5、G26和G3各占23%、28.2%、5.1%和2.7%。扩增出的59个P型中以P[13]型为主,占40.7%,其次为P[6]、P[23]和P[1]型,分别占30.5%、23.7%和5.1%。30株毒株成功鉴定出G/P基因型,G9P[23]（23.3%）为优势组合基因型。其他基因型为G4P[6](16.7%)、G9P[13](13.3%)、G5P[23](10%)、G5P[13](10%)、G9P[6](6.7%)、G26P[13](6.7%)、G4P[13](6.7%)、G4P[23](3.3%)和G3P[13](3.3%)。基因型G5P[23]、G4P[13]、G9P[6]、G26P[13]和G4P[23]为国内首次鉴定。此外,重组分析表明4株毒株在VP7或VP4基因上存在重组现象。【结论】四川地区腹泻仔猪粪便中RVA的流行率高且基因型复杂,优势组合基因型为G9P[23]。该结果丰富了四川地区RVA的流行病学资料,对四川地区猪RVA的防控提供了重要参考。

关键词: 猪A群轮状病毒, 分子流行病学, 基因组研究, 重组

Abstract:

【Objective】The aim of this study was to investigate the prevalence and molecular characterization of porcine group A rotavirus (RVA) in large-scale pig farms in Sichuan, to provid a theoretical basis for the development for porcine RVA vaccine. 【Method】 From 2017 to 2019, the 303 samples were collected from 40 pig farms in 14 regions of Sichuan province. Prevalence of RVA was detected by real-time RT-PCR method, and RVA positive samples were typed by RT-PCR. At the same time, the whole genomes of RVA VP4 and VP7 gene were amplified from some positive samples. The genotypes of the corresponding strains were determined by RotaC^2.0 classification tool. Sequence homology was analyzed by MegAlign. Phylogenetic tree was constructed by Neighbor-Joining method through MEGA 7.0. SimPlot and RDP4 softwares were applied for recombination analysis. 【Result】 Of the 303 samples examined, 32.34% (98/303, 95%CI=27.1%-37.9%) were positive for RVA. Among the 39 G types, G9 (41%) was the dominant genotype, while G4, G5, G26, and G3 were detected in 23%, 28.2%, 5.1% and 2.7%, respectively. The P[13] genotype (40.7%) was dominant among the 59 P types, followed by P[6], P[23] and P[1] in 30.5%, 23.7% and 5.1%, respectively. Furthermore, the 30 strains successfully identified the G/P combination genotype, and the dominant combination genotype was G9P[23] (23.3%), the other combinations were G4P[6] (16.7%), G9P[13] (13.3%), G5P[23] (10%), G5P[13] (10%), G9P[6] (6.7%), G26P[13] (6.7%), G4P[13] (6.7%), G4P[23] (3.3%) and G3P[13] (3.3%). Notably, the G5P[23], G4P[13], G9P[6], G26P[13] and G4P[23] were first identified in China. In addition, the recombination analysis showed that four strains had recombination on VP7 or VP4 genes. 【Conclusion】 The results demonstrated that the prevalence of RVA in diarrhea piglet feces was high and the genotypes were complex in Sichuan. The dominant genotype of RVAs was G9P[23]. The results of this study enriched the epidemiological data of RVA and provided an important reference for the prevention and control of porcine RVA in Sichuan province.

Key words: porcine group A rotavirus, molecular epidemiology, genomic research, recombination

周群,陈小飞,阚蕊慈,李玉,曹慧,彭艳伶,张斌. 2017-2019年四川地区猪A群轮状病毒的分子流行病学调查[J]. 中国农业科学, 2021, 54(5): 1063-1072.

Qun ZHOU,XiaoFei CHEN,RuiCi KAN,Yu LI,Hui CAO,YanLing PENG,Bin ZHANG. Molecular Epidemiological Investigation of Porcine Group A Rotavirus in Sichuan from 2017 to 2019[J]. Scientia Agricultura Sinica, 2021, 54(5): 1063-1072.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2021.05.017

https://www.chinaagrisci.com/CN/Y2021/V54/I5/1063

图/表 6

表1

图1

表2

图2

表3

图3

参考文献 31

[1]	THEINGI W M, HLAING M T, YE M K, KHIN M A, MO M W, HTIN L, THIN T S, WIN M, KHIN K O, KYAW Z T. Sentinel surveillance for rotavirus in children <5 years of age admitted for Diarrheal illness to Yangon Children's Hospital, Myanmar, 2009-2014. Vaccine, 2018,36(51):7832-7835. doi: 10.1016/j.vaccine.2017.11.002. doi: 10.1016/j.vaccine.2017.11.002 pmid: 29274701
[2]	HOSHINO Y, SERENO M M, MIDTHUN K, FLORES J, KAPIKIAN A Z, CHANOCK R M. Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proceedings of the National Academy of Sciences of the United States of America, 1985,82(24):8701-8704. doi: 10.1073/pnas.82.24.8701.
[3]	MIHALOV K E, GELLÉRT Á, MARTON S, FARKAS S L, FEHÉR E, OLDAL M, JAKAB F, MARTELLA V, BÁNYAI K. Candidate new rotavirus species in sheltered dogs, Hungary. Emerging Infectious Diseases, 2015,21(4):660-663. doi: 10.3201/eid2104.141370. doi: 10.3201/eid2104.141370 pmid: 25811414
[4]	MATTHIJNSSENS J, OTTO P H, CIARLET M, DESSELBERGER U, VAN R M, JOHNE R. VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation. Archives of Virology, 2012,157(6):1177-1182. doi: 10.1007/s00705-012-1273-3. doi: 10.1007/s00705-012-1273-3
[5]	TONIETTI P O, HORA A S, SILVA F D F, RUIZ V L A, GREGORI F. Phylogenetic analyses of the VP4 and VP7 genes of porcine group A rotaviruses in Sao Paulo State, Brazil: first identification of G5P[23] in piglets. Journal of Clinical Microbiology, 2013,51(8):2750-2753. doi: 10.1128/JCM.01175-13. pmid: 23761160
[6]	VLASOVA A N, AMIMO J O, SAIF L J. Porcine Rotaviruses: Epidemiology, immune responses and control strategies. Viruses, 2017,9(3):48. doi: 10.3390/v9030048. doi: 10.3390/v9030048
[7]	KOZYRA I, KOZYRA J, DORS A, RZEŻUTKA A. Molecular characterisation of porcine group A rotaviruses: Studies on the age-related occurrence and spatial distribution of circulating virus genotypes in Poland. Veterinary Microbiology, 2019,232:105-113. doi: 10.1016/j.vetmic.2019.03.026. pmid: 31030833
[8]	WAKUDA M, IDE T, SASAKI J, KOMOTO S, ISHII J, SANEKATA T, TANIGUCHI K. Porcine rotavirus closely related to novel group of human rotaviruses. Emerging Infectious Diseases, 2011,17(8):1491-1493. doi: 10.3201/eid1708.101466. pmid: 21801631
[9]	MALASAO R, KHAMRIN P, KUMTHIP K, USHIJIMA H, MANEEKARN N. Complete genome sequence analysis of rare G4P[6] rotavirus strains from human and pig reveals the evidence for interspecies transmission. Infection, Genetics and Evolution, 2018,65:357-368. doi: 10.1016/j.meegid.2018.08.019. pmid: 30144568
[10]	GHOSH S, VARGHESE V, SAMAJDAR S, BHATTACHARYA S K, KOBAYASHI N, NAIK T N. Evidence for independent segregation of the VP6- and NSP4- encoding genes in porcine group A rotavirus G6P[13] strains. Archives of Virology, 2007,152(2):423-429. doi: 10.1007/s00705-006-0848-2. doi: 10.1007/s00705-006-0848-2
[11]	AGBEMABIESE C A, NAKAGOMI T, GAUCHAN P, SHERCHAND J B, PANDEY B D, CUNLIFFE N A, NAKAGOMI O. Whole genome characterisation of a porcine-like human reassortant G26P[19] Rotavirus A strain detected in a child hospitalised for diarrhoea in Nepal, 2007. Infection, Genetics and Evolution, 2017,54:164-169. doi: 10.1016/j.meegid.2017.06.026. doi: 10.1016/j.meegid.2017.06.026
[12]	GREENBERG H, MCAULIFFE V, VALDESUSO J, WYATT R, FLORES J, KALICA A, HOSHINO Y, SINGH N. Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infection and Immunity, 1983,39(1):91-99. doi: 10.1128/ IAI.39.1.91-99. doi: 10.1128/IAI.39.1.91-99.1983 pmid: 6185436
[13]	PAPP H, LÁSZLÓ B, JAKAB F, GANESH B, DE G S, MATTHIJNSSENS J, CIARLET M, MARTELLA V, BÁNYAI K. Review of group A rotavirus strains reported in swine and cattle. Veterinary Microbiology, 2013,165:190-199. doi: 10.1016/j.vetmic. 2013.03.020. doi: 10.1016/j.vetmic.2013.03.020 pmid: 23642647
[14]	MATTHIJNSSENS J, CIARLET M, MCDONALD S M, ATTOUI H, BÁNYAI K, BRISTER J R, BUESA J, ESONA M D, ESTES M K, GENTSCH J R, et al. Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Archives of Virology, 2011,156(8):1397-1413.doi: 10.1007/s00705- 011-1006-z. doi: 10.1007/s00705-011-1006-z
[15]	JING Z, ZHANG X, SHI H, CHEN J, SHI D, DONG H, FENG L. A G3P[13] porcine group A rotavirus emerging in China is a reassortant and a natural recombinant in the VP4 gene. Transboundary and Emerging Diseases, 2018,65(2):e317-e328. doi: 10.1111/tbed.12756. doi: 10.1111/tbed.12756 pmid: 29148270
[16]	曹恭貌, 张斌, 岳华, 费磊, 任玉鹏. 四川部分猪场PEDV、TGEV、GARV和PKV感染状况调查. 动物医学进展, 2016,37(1):118-122.
	CAO G M, ZHANG B, YUE H, FEI L, REN Y P. Investigation of PEDV, TGEV, GARV and PKV infection in some pig farms in Sichuan Province. Progress in Veterinary Medicine, 2016,37(1):118-122. (in Chinese)
[17]	杨文宇, 周远成, 韩燕, 陈蕾, 廖春燕, 付梦瑾, 季洪伟, 蔡雨涵, 朱玲, 徐志文, 郭万柱. 猪A群轮状病毒和C群轮状病毒以及猪星状病毒多重RT-PCR同时检测方法的建立及应用. 中国兽医科学, 2014,44(4):394-400.
	YANG W Y, ZHOU Y C, HAN Y, CHEN L, LIAO C Y, FU M J, JI H W, CAI Y H, ZHU L, XU Z W, GUO W Z. Establishment and clinical application of a multiplex reverse transcription-PCR for simultaneous detection of porcine group A rotrvirus, porcine group C rotavirus and porcine astrovirus. Chinese Veterinary Science, 2014,44(4):394-400. (in Chinese)
[18]	XUE R, TIAN Y, ZHANG Y, ZHANG M, LI Z, CHEN S, LIU Q. Diversity of group A rotavirus of porcine rotavirus in Shandong province China. Acta Virologica, 2018,62(3):229-234. doi: 10.4149/ av_2018_216. doi: 10.4149/av_2018_216 pmid: 30160138
[19]	周玲, 陈桂华, 伍子娴, 麦凯杰, 李迪, 王胜阳, 马静云, 张祥斌. 广东地区2016-2017年规模化猪场腹泻病原调查分析. 中国兽医杂志, 2017, 53(10): 3-5+9+49.
	ZHOU L, CHEN G H, WU Z X, MAI K J, LI D, WANG S Y, MA J Y, ZHANG X B. Investigation on porcine diarrhea pathogens in Guangdong province form 2016 to 2017. Chinese Journal of Veterinary Medicine, 2017, 53(10): 3-5+9+49.(in Chinese)
[20]	库旭钢, 张坤, 刘羽茜, 何启盖. 猪A群轮状病毒的分离与鉴定. 畜牧兽医学报, 2012,43(2):275-281.
	KU X G, ZHANG K, LIU X X, HE Q G. Isolation and identification of group A porcine rotavirus. Acta Veterinaria et Zootechnica Sinica, 2012,43(2):275-281. (in Chinese)
[21]	李玉, 穷达, 张敏, 阚蕊慈, 汤承, 岳华, 张斌. 猪A群轮状病毒RVA/Pig-tc/CHN/SWU-1C/2018/G9P[13]株的分离与鉴定. 中国预防兽医学报, 2019,41(11):1170-1173.
	LI Y, QIONG D, ZHANG M, KAN R C, TANG C, YUE H, ZHANG B. Isolation and identification of the porcine group A rotavirus strain of RVA/Pig-tc/CHN/SWU-1C/2018/G9P[13]. Chinese Journal of Preventive Veterinary Medicine, 2019,41(11):1170-1173. (in Chinese)
[22]	ZHANG H, ZHANG Z, WANG Y F, WANG X, XIA M Q, WU H. Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China. Archives of Virology, 2015,160(5):1333-1338. doi: 10.1007/s00705-015- 2347-9.
[23]	AMIMO J O, VLASOVA A N, SAIF L J. Detection and genetic diversity of porcine group A rotaviruses in historic (2004) and recent (2011 and 2012) swine fecal samples in Ohio: Predominance of the G9P[13] genotype in nursing piglets. Journal of Clinical Microbiology, 2013,51(4), 1142-1151. doi: 10.1128/JCM.03193-12.
[24]	DISTEFANO D J, KRAIOUCHKINE N, MALLETTE L, MALIGA M, KULNIS G, KELLER P M, CLARK H F, SHAW A R. Novel rotavirus VP7 typing assay using a one-step reverse transcriptase PCR protocol and product sequencing and utility of the assay for epidemiological studies and strain characterization, including serotype subgroup analysis. Journal of Clinical Microbiology, 2005,43(12):5876-5880. doi: 10.1128/JCM.43.12.5876-5880. pmid: 16333070
[25]	YAN N, TANG C, KAN R C, FENG F, YUE H. Genome analysis of a G9P[23] group A rotavirus isolated from a dog with diarrhea in China. Infection, Genetics and Evolution, 2019,70:67-71. doi: 10.1016/j. meegid.2019.02.020. pmid: 30796978
[26]	WANG Y H, PANG B B, ZHOU X, GHOSH S, TANG W F, PENG J S, HU Q, ZHOU D J, KOBAYASHI N. Complex evolutionary patterns of two rare human G3P[9] rotavirus strains possessing a feline/canine-like H6 genotype on an AU-1-like genotype constellation. Infection, Genetics and Evolution, 2013, 1:103-112. doi: 10.1016/j. meegid.2013.01.016.
[27]	TIMURKAN M Ö, ALKAN F. Identification of rotavirus A strains in small ruminants: first detection of G8P[1] genotypes in sheep in Turkey. Archives of Virology, 2020,165(2):425-431. doi: 10.1007/ s00705-019-04476-7. pmid: 31828508
[28]	MISHRA N, RESLAN L, EL-HUSSEINI M, RAOOF H, FINIANOS M, GUO C, THAKKAR R, INATI A, DBAIBO G, LIPKIN W I, ZARAKET H. Full genome characterization of human G3P[6] and G3P[9] rotavirus strains in Lebanon. Infection, Genetics and Evolution, 2020,78:104-133. doi: 10.1016/j.meegid.2019.104133.
[29]	LI D D, DUAN Z J, ZHANG Q, LIU N, XIE Z P, JIANG B M, STEELE D, JIANG X, WANG Z S, FANG Z Y. Molecular characterization of unusual human G5P[6] rotaviruses identified in China. Journal of Clinical Virology, 2008,42(2):141-148. doi: 10. 1016/j.jcv.2007.12.013. doi: 10.1016/j.jcv.2007.12.013 pmid: 18304868
[30]	RAMANI S, ITURRIZA G M, JANA A K, KURUVILLA K A, GRAY J J, BROWN D W, KANG G. Whole genome characterization of reassortant G10P[11] strain (N155) from a neonate with symptomatic rotavirus infection: Identification of genes of human and animal rotavirus origin. Journal of Clinical Virology, 2009,45(3):237-244. doi: 10.1016/j.jcv.2009.05.003.
[31]	CHEN D Y, ZHOU L, TIAN Y M, WU X, FENG L, ZHANG X P, LIU Z H, PANG S R, KANG R M, YU J F, YE Y G, WANG H N, YANG X. Genetic characterization of a novel G9P[23] rotavirus A strain identified in southwestern China with evidence of a reassortment event between human and porcine strains. Archives of Virology, 2019,164(4):1229-1232. doi: 10.1007/s00705-019-04188-y. pmid: 30810805

地区 Region	猪场个数 Farm	样本数 Number of samples	RVA阳性数 Positive number	G型 G genotype	P 型 P genotype	G/P组合型 G/P combination genotype
眉山Meishan	6	62	14	G3,G4,G9	P[1],P[13],P[23]	G3P[13],G4P[23],G9P[23],G9P[13]
绵阳Mianyang	5	34	24	G5,G9	P[23]	G9P[23],G5P[23]
泸州Luzhou	5	13	3	G9	P[1],P[13]	G9P[13]
西昌Xichang	5	25	8		P[1],P[13]
成都Chengdu	4	49	9	G9	P[13],P[23]	G9P[13],G9P[23]
雅安Yaan	4	13	3	G9	P[13]	G9P[13]
内江Neijiang	3	18	2	G9	P[6]	G9P[6]
宜宾Yibin	2	7	5	G5	P[13],P[23]	G5P[23],G5P[13]
乐山Leshan	1	4	2	G4	P[13]	G4P[13]
遂宁Suining	1	18	4	G9,G26	P[13],P[23]	G9P[23],G26P[13]
南充Nanchong	1	3	3	G9	P[13]	G9P[13]
资阳Ziyang	1	18	2	G4	P[6]	G4P[6]
广元Guangyuan	1	36	19	G4,G5	P[6],P[13]	G4P[6],G5P[13]
攀枝花Panzhihua	1	3	0
共计Total	40	303	98

毒株 Strain	同源性最高的毒株 Strain with highest homology	国家及宿主 Country and host	年份 Year	同源性 Homology
SCMS-71	SCQL-5-2（MG066590）	中国/猪源China/pig	2018	99.9%
SCLS-18,SCLS-20	MOZ/21205（KP222851）	莫桑比克/人源Mozambique/human	2011	96.9%,97.4%
SCZY-165,SCZY-166	ZT-11D-T101（KM247280）	中国/人源China/human	2011	98.9%,98.3%
SCMS-69	GUB71（AB573647）	日本/猪源Japan/pig	2006	95.6%
SCGY-193,SCGY-198,SCGY-199,SCGY-201	R1954（KF726066）	中国/人源China/human	2013	95.1%
6株G5型（SCJY）,SCGY-192 6 strains of G5 （SCJY）	HLJqqhe-1（JX498960）	中国/猪源China/pig	2011	97.6%-98.1%
SCYB-Y1,SCYB-Y2	LL3354（EF159575）	中国/人源China/human	2000	93.9%,94.2%
SCYB-C2,SCYB-C3	103-P-001-1-0126（MK227837）	中国/猪源China/pig	2014	93.7%
4株G9型（SCJY） 4 strains of G9 （SCJY）	LLP48（KJ126835）	中国/猪源China/pig	2008	97.4%-97.6%
SCRS-142,SCSN-96	HBHP（KX831114）	中国/猪源China/pig	2016	98.3%
SCDY-219	GXqz-2（JX498942）	中国/猪源China/pig	2011	97.5%
SCLZ-FB,SCYA-C7	ISO31（DQ117937）	印度/人源India/human	2008	96.2%,96.5%
SCMY-28,SCMS-153,SCDY-211, SCDY-210,SCDY-209	CQ（KX831100）	中国/猪源China/pig	2016	95.9%-97.1%
SCSN-93,SCSN-94	07N1760（LC208008）	尼泊尔/猪源Nepal/pig	2007	96.1%
SCLJ-C18,SCLJ-C20	TA-1-2（KT820779）	中国/猪源China/pig	2014	85.1%

毒株 Strain	同源性最高的毒株 Strain with highest homology	国家及宿主 Country and host	年份 Year	同源性 Homology
SCMS-152,SCLZ-35,SCXC-83	A5-10（LC133517）	泰国/牛源Thailand/cow	1988	93.4%
15株P[6]型（SCGY）15 strains of P[6] （SCGY）	GUB88（AB573872）	日本/猪源Japan/pig	2006	92.6%-98.6%
SCLJ-C18,SCLJ-C20,SCZY-165	R1207（LC389888）	斯里兰卡/人源Sri Lanka/human	2009	95.7%-98.6%
SCXC-89,SCXC-85,SCXC-84,SCRS-142,SCGY-192	CMP178（DQ536362）	泰国/猪源Thailand/pig	2008	94.5%-96.8%
SCYB-Y1,SCQL-54,SCQL-55,SCMS-71,SCDY-91	SCMY（MH320795）	中国/猪源China/pig	2018	79.1%-98.8%
SCLZ-FB	FGP36（AB573878）	日本/猪源Japan/pig	2009	95.1%
SCSN-93,SCSN-94	LNCY（MF462324）	中国/猪源China/pig	2016	98.3%
SCYA-C7,SCRS-131,SCLS-18,SCLS-20, SCYA-H2,SCYA-H3,SCXC-90,SCYB-Y2	SWU-1C(MK410284)	中国/猪源China/pig	2018	92.5%-97.7%
SCDY-210,SCSN-140,SCMS-151	SCLS-R3（MK597985）	中国/人源China/human	2018	92.6%-97.0%
SCYB-C2,SCYB-C3	14175-24（KX363359）	越南/猪源Viet Nam/pig	2012	95.3%
SCSN-96	SC11（MH624176）	中国/猪源China/pig	2017	97.4%
SCMS-153,SCMS-68,SCMS-69,SCDY-211, 7株P[23]型（SCJY）7 strains of P[23] （SCJY）	HLJ/15/1（KU886316）	中国/猪源China/pig	2015	92.1-96.4%

2017-2019年四川地区猪A群轮状病毒的分子流行病学调查

Molecular Epidemiological Investigation of Porcine Group A Rotavirus in Sichuan from 2017 to 2019

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 31

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	赵凌, 张勇, 魏晓东, 梁文化, 赵春芳, 周丽慧, 姚姝, 王才林, 张亚东. 利用高密度Bin图谱定位水稻抽穗期剑叶叶绿素含量QTL[J]. 中国农业科学, 2022, 55(5): 825-836.
[2]	郝静,李秀坤,崔顺立,邓洪涛,侯名语,刘盈茹,杨鑫雷,穆国俊,刘立峰. 花生每荚种子数相关性状QTL的定位[J]. 中国农业科学, 2022, 55(13): 2500-2508.
[3]	张静远,缪发明,陈腾,李敏,扈荣良. 非洲猪瘟实时荧光RPA诊断方法建立及应用[J]. 中国农业科学, 2022, 55(1): 197-207.
[4]	段玉,许建建,马志敏,宾羽,周常勇,宋震. 柑橘叶斑驳病毒的逆转录重组酶聚合酶扩增检测[J]. 中国农业科学, 2021, 54(9): 1904-1912.
[5]	孟鑫浩,邓洪涛,李丽,崔顺立,Charles Y.Chen,侯名语,杨鑫雷,刘立峰. 栽培种花生株型相关性状的QTL定位[J]. 中国农业科学, 2021, 54(8): 1599-1612.
[6]	郭淑青,宋慧,杨清华,高金锋,高小丽,冯佰利,杨璞. 谷子株高及穗部性状主基因+多基因混合遗传模型分析[J]. 中国农业科学, 2021, 54(24): 5177-5193.
[7]	李松美,仇雨歌,陈胜男,王晓萌,王春生. CRISPR/Cas9介导的绵羊示踪脐带间充质干细胞系的建立[J]. 中国农业科学, 2021, 54(2): 400-411.
[8]	潘丽媛,贺建波,赵晋铭,王吴彬,邢光南,喻德跃,张小燕,李春燕,陈受宜,盖钧镒. RTM-GWAS方法应用于大豆RIL群体百粒重QTL检测的功效[J]. 中国农业科学, 2020, 53(9): 1730-1742.
[9]	刘再东,孟珊,贺建波,邢光南,王吴彬,赵团结,盖钧镒. 大豆重组自交系群体异黄酮含量QTL连锁定位与关联定位的比较研究[J]. 中国农业科学, 2020, 53(9): 1756-1772.
[10]	赵久然, 李春辉, 宋伟, 刘新香, 王元东, 张如养, 王继东, 孙轩, 王夏青. 玉米骨干自交系京2416杂种优势及遗传重组解析[J]. 中国农业科学, 2020, 53(22): 4527-4536.
[11]	秦艳红,王永江,王爽,乔奇,田雨婷,张德胜,张振臣. 甘薯羽状斑驳病毒O株系和RC株系中国分离物全基因组序列分析及其遗传特征[J]. 中国农业科学, 2020, 53(11): 2207-2218.
[12]	贾云慧,许程志,隋金钰,吴运谱,许榜丰,陈艳,杨焕良,乔传玲,陈化兰. 欧亚类禽型H1N1猪流感病毒HA蛋白的表达及免疫原性评估[J]. 中国农业科学, 2019, 52(5): 930-938.
[13]	陈文凤,王红芳,刘振国,张卫星,郗学鹏,胥保华. 中华蜜蜂Apidaecin的重组表达及其抗菌活性[J]. 中国农业科学, 2019, 52(4): 767-776.
[14]	毛娅卿,张兵,王团结,侯力丹,黄小洁,刘丹,赵俊杰,李启红,王乐元,李俊平,杨承槐. UL56基因下游3513bp对鸭肠炎病毒生物特性的影响[J]. 中国农业科学, 2019, 52(23): 4390-4397.
[15]	孙莹,张兵,李岭,黄小洁,侯力丹,刘丹,李启红,李俊平,王乐元,李慧姣,杨承槐. 表达H9亚型禽流感病毒HA基因重组鸭肠炎病毒的构建[J]. 中国农业科学, 2019, 52(23): 4398-4405.