糜子资源耐盐性评价与盐胁迫生理响应

doi:10.3864/j.issn.0578-1752.2019.22.011

中国农业科学 ›› 2019, Vol. 52 ›› Issue (22): 4066-4078.doi: 10.3864/j.issn.0578-1752.2019.22.011

糜子资源耐盐性评价与盐胁迫生理响应

袁雨豪¹,杨清华¹,党科¹,杨璞¹,高金锋¹,高小丽¹,王鹏科¹,陆平²,刘敏轩²,冯佰利¹()

1 西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室,陕西杨凌 712100
2 中国农业科学院作物科学研究所,北京 100081

收稿日期:2019-06-11 接受日期:2019-07-31 出版日期:2019-11-16 发布日期:2019-11-16
通讯作者: 冯佰利
作者简介:袁雨豪,E-mail：yuanyuhao126@163.com
基金资助:
国家谷子高粱产业技术体系(CARS-13.5-06-A26);国家“十二五”科技支撑计划(2014BAD07B03);国家自然科学基金(31371529);陕西省2017年省级现代农作物种业项目(20171010000004);陕西省小杂粮产业技术体系项目(NYKJ-2018-YL19)

Salt-Tolerance Evaluation and Physiological Response of Salt Stress of Broomcorn Millet (Panicum miliaceum L.)

YUAN YuHao¹,YANG QingHua¹,DANG Ke¹,YANG Pu¹,GAO JinFeng¹,GAO XiaoLi¹,WANG PengKe¹,LU Ping²,LIU MinXuan²,FENG BaiLi¹()

1 College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi;
2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081

Received:2019-06-11 Accepted:2019-07-31 Online:2019-11-16 Published:2019-11-16
Contact: BaiLi FENG

摘要/Abstract

摘要：

【目的】评价糜子资源对盐胁迫的耐受性,探究盐胁迫下不同耐盐性糜子品种形态结构变化及生理响应,建立糜子耐盐性鉴定的指标体系。【方法】在人工气候箱内用质量分数1% NaCl溶液胁迫,对100份糜子资源萌发期发芽势、发芽率、胚芽鞘长、根长、芽长、根重、芽重及苗期生理指标进行测定。运用相关性分析、主成分分析、聚类分析及苗期对盐胁迫的生理响应进行综合评价,评估糜子品种对盐胁迫的耐受性。【结果】在质量分数1% NaCl胁迫处理下,糜子品种萌发期幼苗芽长小于对照,但不同品种降低幅度存在显著差异。相关性分析表明,各萌发期指标相对值之间存在显著或极显著正相关,与相对盐害率存在显著或极显著负相关。主成分分析结果表明,根鲜重、发芽指数、发芽率在萌发因子中的负荷量较大,可作为萌发期糜子耐盐性筛选的主要鉴定指标。聚类分析表明,伊选大红糜、污咀黍、白黍等11个品种为高度耐盐品种资源,呼盟黑粘糜、小黑黍、临河双粒黍、陕78等4个品种为高度盐敏感品种资源。耐盐性品种与盐敏感性品种在形态结构和生理指标上存在较大差异。扫描电镜观察发现,随着盐胁迫时间的延长,耐盐性品种叶表面出现盐囊泡并增多变大,气孔张开度变化不明显。盐敏感性品种气孔关闭,叶片表面蜡质增多粗糙,气孔保卫细胞受损破裂出现凋亡。透射电镜显示,随着盐胁迫时间的增加,耐盐性品种叶绿体外部形态变化不明显,在胁迫12 h后仍能维持正常的内部结构。而盐敏感性品种叶绿体由椭球形或椭球形变成球形且淀粉粒增多,叶绿体外被膜逐渐解体,基粒排列紊乱随机分布,类囊体膨胀,甚至解体消失。耐盐性品种相对电导率增加幅度小于盐敏感性品种,而叶绿素荧光参数（Fv/Fm、Y(Ⅱ)和NPQ）降低幅度小于盐敏感性品种。【结论】质量分数1% NaCl溶液可作为糜子萌发期耐盐性鉴定的适宜盐浓度。发芽势、发芽率、发芽指数可作为糜子萌发期耐盐性鉴定的指标,扫描电镜下气孔的状态、透射电镜下叶绿体超微结构的变化可作为糜子耐盐性鉴定的细胞学指标,相对电导率和叶绿素参数可作为糜子耐盐性鉴定的生理指标。

关键词: 糜子, NaCl胁迫, 耐盐性鉴定, 耐盐生理响应

Abstract:

【Objective】 To evaluate the tolerance of broomcorn millet cultivars to salt stress, explore the physiological responses of different salt-tolerant broomcorn millet to salt stress, and establish the salt tolerance identification index system of broomcorn millet, the salt tolerance of broomcorn millet was studied. 【Method】 The growth parameters of 100 genotypes of broomcorn millet at germination stage and the physiological parameters of different salt-tolerant broomcorn millet at seedling stage were measured under salt stress. The tolerance of each variety to salt stress was evaluated by correlation analysis, principal component analysis, cluster analysis and physiological responses to salt stress. 【Result】 the results showed that the measured values of each shoot in the germination stage under 1% NaCl solution stress were lower than that of the control. Correlation analysis showed that there was a significant correlation in the different germination indexes, and there was a significant negative correlation with the relative salt damage rate. The principal component analysis results showed root fresh weight, germination index and germination rate were the larger load in germination factors and these indexes were considered as the main indexes to identify salt tolerance of broomcorn millet germination. The 100 broomcorn millet cultivars were sorted four groups according to the cluster analysis. The cluster analysis results showed eleven cultivars such as Yixuandahongmi, Wujushu, Baishu etc. were classified into salt-tolerant cultivar group, four cultivars including Humengheinianmi, Xiaoheishu, Linheshuanglishu, and Shaan78 were classified into salt sensitive group. There were great differences between salt tolerant cultivars and salt sensitive cultivars in morphological structure and physiological indexes. The scanning electron microscope results showed salt bladders appeared on the surface of salt-tolerant cultivars, the stomatal aperture size was barely changed. The salt-sensitive cultivar showed the stomata were closed in salt-sensitive broomcorn millet cultivar, and the surface of the leaf become rough, and the guard cells death occurred. With the increase of treatment time, the salt tolerant cultivar chloroplast changed from ellipsoid to spherical and starch granules increase, membrane disrupted, grana lamella arranged loosely, the thylakoids swollen and even disintegrate. With the days of salt stress increasing, the relative conductivity were increased. However, the relative conductivity in salt-tolerant broomcorn millet cultivar were lower than salt-sensitive broomcorn millet cultivar. The decreasing of Fv/Fm、Y(Ⅱ) and NPQ in salt-tolerant broomcorn millet cultivar were less than salt-sensitive broomcorn millet cultivar. 【Conclusion】 1% NaCl solution could be used as the suitable concentration of salt tolerance in broomcorn millet. The results showed that salt tolerance evaluation of broomcorn millet was affected with multiple indexes, and root fresh weight, germination index and germination rate could be used as the evaluation indexes of salt tolerance in germination stage. Stomatal state and chloroplast structures could be used as the cytology indexes of salt tolerance evaluation. The relative conductivity and chlorophyll fluorescence coefficient could be used as the physiology indexes of salt tolerance evaluation.

Key words: broomcorn millet, NaCl stress, salt tolerance screening, physiological response

袁雨豪, 杨清华, 党科, 杨璞, 高金锋, 高小丽, 王鹏科, 陆平, 刘敏轩, 冯佰利. 糜子资源耐盐性评价与盐胁迫生理响应[J]. 中国农业科学, 2019, 52(22): 4066-4078.

YUAN YuHao, YANG QingHua, DANG Ke, YANG Pu, GAO JinFeng, GAO XiaoLi, WANG PengKe, LU Ping, LIU MinXuan, FENG BaiLi. Salt-Tolerance Evaluation and Physiological Response of Salt Stress of Broomcorn Millet (Panicum miliaceum L.)[J]. Scientia Agricultura Sinica, 2019, 52(22): 4066-4078.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2019.22.011

https://www.chinaagrisci.com/CN/Y2019/V52/I22/4066

图/表 10

表1

图1

表2

表3

表4

表5

图2

图3

图4

图5

参考文献 49

[1]	IVITS E V A, CHERLET M, TÓTH T, LEWIŃSKA K E, TÓTH G . Characterisation of productivity limitation of salt-affected lands in different climatic regions of Europe using remote sensing derived productivity indicators. Land Degradation & Development, 2013,24(5):438-452.
[2]	GUPTA B, HUANG B R . Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. International Journal of Genomics, 2014(1):701596.
[3]	FLOWERS T J, FLOWERS S A . Why does salinity pose such a difficult problem for plant breeders? Agricultural Water Management, 2005,78(1/2):15-24.
[4]	GUO H L, WANG Y, LI D D, CHEN J B, ZONG J Q, WANG Z Y, CHEN X, LIU J X . Growth response and ion regulation of seashore paspalum accessions to increasing salinity. Environmental & Experimental Botany, 2016,131:137-145.
[5]	蒋静, 翟登攀, 张超波 . 灌溉施肥水平对盐渍化农田水盐分布及玉米产量的影响. 应用生态学报, 2019,30(4):1207-1217.
	JIANG J, ZHAI D P, ZHANG C B . Effects of irrigation and fertilizer levels on the distribution of water and salt in saline field and maize yield. Chinese Journal of Applied Ecology, 2019,30(4):1207-1217. (in Chinese)
[6]	李彬, 王志春, 孙志高, 陈渊, 杨福 . 中国盐碱地资源与可持续利用研究. 干旱地区农业研究, 2005,23(2):154-158.
	LI B, WANG Z C, SUN Z G, CHEN Y, YANG F . Resources and sustainable resource exploitation of salinized land in China. Agricultural Research in the Arid Areas, 2005,23(2):154-158. (in Chinese)
[7]	QADIR M, QUILLEROU E, NANGIA V, MUTAZA G . Economics of salt‐induced land degradation and restoration. Natural Resources Forum, 2014,38(4):282-295.
[8]	JESUS J M, DANKO A S, FIUZA A, BORGES M T . Phytoremediation of salt-affected soils: A review of processes, applicability, and the impact of climate change. Environmental Science and Pollution Research, 2015,22(9):6511-6525.
[9]	ZHU J K . Abiotic stress signaling and responses in plants. Cell, 2016,167(2):313-324.
[10]	YANG Y Q, GUO Y . Elucidating the molecular mechanisms mediating plant salt‐stress responses. New Phytologist, 2018,217(2):523-539.
[11]	YANG Y Q, GUO Y . Unraveling salt stress signaling in plants. Journal of Integrative Plant Biology, 2018,60(9):58-66.
[12]	马佩勇, 边小峰, 郭小丁, 贾赵东, 禹阳, 谢一芝 . 甘薯全生育期耐盐种质筛选与耐盐性评价. 植物遗传资源学报, 2018,19(3):174-181.
	MA P Y, BIAN X F, GUO X D, JIA Z D, YU Y, XIE Y Z . Evaluation and screening for salt-tolerant sweetpotato accessions during growth period. Journal of Plant Genetic Resources, 2018,19(3):174-181. (in Chinese)
[13]	韩飞, 诸葛玉平, 娄燕宏, 王会, 张乃丹, 何伟, 晁赢 . 63份谷子种质的耐盐综合评价及耐盐品种筛选. 植物遗传资源学报, 2018,19(4):685-693.
	HAN F, ZHUGE Y P, LOU Y H, WANG H, ZHANG N D, HE W, CHAO Y . Evaluation of salt tolerance and screening for salt tolerant accessions of 63 foxtail millet germplasm. Journal of Plant Genetic Resources, 2018,19(4):685-693. (in Chinese)
[14]	YANG Q H, ZHANG P P, QU Y, GAO X L, LIANG J B, YANG P, FENG B L . Comparison of physicochemical properties and cooking edibility of waxy and non-waxy proso millet ( Panicum miliaceum L.). Food Chemistry, 2018,257:271-278.
[15]	ARAB A, BRADARAN R, VAHIDIPOUR T H . Effect of irrigation and mycorrhizal bio-fertilizers on yield and agronomic traits of millet ( Panicum miliaceum L.). International Journal of Agriculture & Crop Sciences, 2013,6(2):103-109.
[16]	YUE H, WANG M, LIU S Y, DU X H, SONG W N, NIE X J . Transcriptome-wide identification and expression profiles of the WRKY transcription factor family in Broomcorn millet ( Panicum miliaceum L.). BMC Genomics, 2016,17(1):343.
[17]	ZHANG D Z, PANHWAR R B, LIU J J, GONG X W, LIANG J B, LIU M X, LU P, GAO X L, FENG B L . Morphological diversity and correlation analysis of phenotypes and quality traits of proso millet ( Panicum miliaceum L.) core collections. Journal of Integrative Agriculture, 2019,18(5):958-969.
[18]	XIE F L, WANG Q L, SUN R R, ZHANG B H . Deep sequencing reveals important roles of microRNAs in response to drought and salinity stress in cotton. Journal of Experimental Botany, 2014,66(3):789-804.
[19]	SUN Y, XU W, JIA Y B, WANG M C . The wheat TaGBF1 gene is involved in the blue‐light response and salt tolerance. The Plant Journal, 2015,84(6):1219-1230.
[20]	HUANG Y, ZHANG X X, LI Y H, DING J Z, DU H M, ZHAO Z, ZHOU L N, LIU C, GAO S B, CAO M J, LU Y L, ZHANG S Z . Overexpression of the Suaeda salsa SsNHX1 gene confers enhanced salt and drought tolerance to transgenic Zea mays. Journal of Integrative Agriculture, 2018,17(12):2612-2623.
[21]	CHEN G, HU Q D, LUO L, YANG T Y, ZHANG S, HU Y B, YU L, XU G H . Rice potassium transporter OsHAK1 is essential for maintaining potassium‐mediated growth and functions in salt tolerance over low and high potassium concentration ranges. Plant, Cell & Environment, 2015,38(12):2747-2765.
[22]	LI W, ZHAO F A, FANG W P, XIE D Y, HOU J N, YANG X J, ZHAO Y M, TANG Z J, NIE L H, LV S P . Identification of early salt stress responsive proteins in seedling roots of upland cotton (Gossypium hirsutum L.) employing iTRAQ-based proteomic technique. Frontiers in Plant Science, 2015,6:732.
[23]	WANG F B, ZHAI H, AN Y Y, SI Z Z, HE S Z, LIU Q C . Overexpression of IbMIPS1 gene enhances salt tolerance in transgenic sweetpotato. Journal of Integrative Agriculture, 2016,15(2):271-281.
[24]	李占成, 张丽丽, 李玮 . 盐胁迫对糜子种子发芽的影响. 作物杂志, 2011(6):122-123.
	LI Z C, ZHANG L L, LI W . Effect of salt stress on germination of gardenia seeds. Crop, 2011(6):122-123. (in Chinese)
[25]	LIU M X, QIAO Z J, ZHANG S, WANG Y Y, LU P . Response of broomcorn millet (Panicum miliaceum L.) genotypes from semiarid regions of China to salt stress. Journal of Integrative Agriculture, 2015,3(1):57-66.
[26]	SHI J P, MA X X, ZHANG J H, ZHOU Y S, LIU M X, HUANG L L, SU S L, ZHANG X B, GAO X, ZHAN W, LI P H, WANG L, LU P, ZHAO H M, SONG W B, LAI J S . Chromosome conformation capture resolved near complete genome assembly of broomcorn millet. Nature Communications, 2019,10(1):464-477.
[27]	ZOU C S, LI L T, MILI D, LI D L, TANG Q M, XIAO L H, RAJPUT S, DENG P, PENG L, JIA W, HUANG R, ZHANG M L, SUN Y D, HU J M, FU X, SCHNABLE P S, CHANG Y X, LI F, ZHANG H, FENG B L, ZHU X G, LIU R Y, SCHNABLE J C, ZHU J K, ZHANG H . The genome of broomcorn millet. Nature Communications, 2019,10(1):436.
[28]	陈宣, 郭海林, 陈静波, 刘建秀 . 结缕草属植物两种耐盐评价方法的比较. 草业科学, 2014,31(6):1052-1057.
	CHEN X, GUO H L, CHEN J B, LIU J X . Comparison of salinity tolerance evaluation system of Zoysia grass. Pratacultural Science, 2014,31(6):1052-1057. (in Chinese)
[29]	张寒, 潘香逾, 王秀华, 李家丽, 姜慧新, 赵岩 . 苜蓿萌发期耐盐性综合评价与耐盐种质筛选. 草地学报, 2018(3):666-672.
	ZHANG H, PAN X Y, WANG X H, LI J L, JIANG H X, ZHAO Y . Comprehensive evaluation of salt tolerance and screening for salt tolerant germplasm of Alfalfa (Medicago) at germination stage. Acta Agrestia Sinica, 2018(3):666-672. (in Chinese)
[30]	李娜娜, 张煜, 王俊峰, 张晓冬, 马玉敏, 丁汉凤 . 栽培大豆种质资源耐盐性的研究进展. 中国农学通报, 2011,27(27):6-11.
	LI N N, ZHANG Y, WANG J F, ZHANG X D, MA Y M, DING H F . Advances in studies of salt tolerance in cultivated soybean. Chinese Agricultural Science Bulletin, 2011,27(27):6-11. (in Chinese)
[31]	张金霞, 董德坤, 胡兴旺, 陈芬, 朱丹华 . 三个大豆品种萌发期和苗期的耐盐性比较. 浙江农业学报, 2016,28(7):1101-1107.
	ZHANG J X, DONG D K, HU X W, CHEN F, ZHU D H . Research on salt tolerance of three soybean varieties at both germination and seedling stage. Acta Agriculturae Zhejiangensis, 2016,28(7):1101-1107. (in Chinese)
[32]	BARTON L, NEWSOME S D, CHEN F H, WANG H, GUILDERSON T P, BETTINGER R . Agricultural origins and the isotopic identity of domestication in northern China. Proceedings of the National Academy of Sciences of the USA, 2009,106(14):5523-5528.
[33]	YUE H, WANG M, LIU S . Transcriptome-wide identification and expression profiles of the WRKY transcription factor family in Broomcorn millet ( Panicum miliaceum L.). BMC Genomics, 2016,17(1):343.
[34]	RAJPUT S G, SANTRA D K, SCHNABLE J . Mapping QTLs for morpho-agronomic traits in Proso millet ( Panicum miliaceum L.): New strategies in plant improvement. Molecular Breeding, 2016,36(4):1-18.
[35]	张洪鹏, 陈德龙, 马强 . 不同糜子品种芽期耐盐性比较. 安徽农业科学, 2016,44(23):11-13.
	ZHANG H P, CHEN D L, MA Q . Comparative study on salt tolerance of different varieties of broomcorn millet at the germination stage. Journal of Anhui Agricultural Sciences, 2016,44(23):11-13. (in Chinese)
[36]	苏国兴, 洪法水 . 桑品种耐盐性的隶属函数法之评价. 江苏农业学报, 2002,18(1):42-47.
	SU G X, HONG F S . Evaluation of salt tolerance for partial mulberry varieties with subordinate function. Jiangsu Journal of Agricultural Sciences, 2002,18(1):42-47. (in Chinese)
[37]	王志春, 梁正伟 . 植物耐盐研究概况与展望. 生态环境, 2003,12(1):106-109.
	WANG Z C, LIANG Z W . Advances of salt tolerance in plants. Journal of Environmental Sciences, 2003,12(1):106-109. (in Chinese)
[38]	刘敏轩, 张宗文, 吴斌, 陆平 . 黍稷种质资源芽、苗期耐中性混合盐胁迫评价与耐盐生理机制研究. 中国农业科学, 2012,45(18):3733-3743.
	LIU M X, ZHANG Z W, WU B, LU P . Evaluation of mixed salt-tolerance at germination stage and seedling stage and the related physiological characteristics of Panicum miliaceum L. Scientia Agricultura Sinica, 2012,45(18):3733-3743. (in Chinese)
[39]	张国伟, 路海玲, 张雷, 陈兵林, 周治国 . 棉花萌发期和苗期耐盐性评价及耐盐指标筛选. 应用生态学报, 2011,22(8):2045-2053.
	ZHANG G W, LU H L, ZHANG L, CHEN B L, ZHOU Z G . Salt tolerance evaluation of cotton at its germinating and seedling stages and selection of related indices. Chinese Journal of Applied Ecology, 2011,22(8):2045-2053. (in Chinese)
[40]	YANG Y Q, GUO Y . Elucidating the molecular mechanisms mediating plant salt-stress responses. New Phytologist, 2018,217(2):523-539.
[41]	GAO W, XU F C, GUO D D, ZHAO J R, LIU J, GUO Y W, SINGH P K, MA X N, LONG L, BOTELLA J R, SONG C P . Calcium- dependent protein kinases in cotton: insights into early plant responses to salt stress. BMC Plant Biology, 2018,18(1):15.
[42]	YANG Y Q, GUO Y . Unraveling salt stress signaling in plants. Journal of Integrative Plant Biology, 2018,60(9):58-66.
[43]	左凤月 . 盐胁迫对3种白刺生长、生理生化及解剖结构的影响[D]. 重庆: 西南大学, 2013.
	ZUO F Y . The effect of NaCl treatment on the seed germination,the seedling growth,the leaf physiology and biochemistry and the anatomical structures of leaf and stem of 3 Nitraria species[D]. Chongqing: Southwest University, 2013. (in Chinese)
[44]	杨美娟, 杨德奎, 李法曾 . 中亚滨藜盐囊泡形态结构与发育研究. 西北植物学报, 2006,26(8):1575-1578.
	YANG M J, YANG D K, LI F Z . Morphological structure and development of salt bladder of Atriplex centralasiatica. Acta Botanica Boreali-Occidentalia Sinica, 2006,26(8):1575-1578. (in Chinese)
[45]	KHAN M S, HEMALATHA S . Biochemical and molecular changes induced by salinity stress in Oryza sativa L. Acta Physiologiae Plantarum, 2016,38(7):1-9.
[46]	ZHO J L, WANG X F, JIAO Y L, QIN Y H, LIU X G, HE K, CHEN C, MA L G, WANG J, XIOMG L Z, ZHANG Q F, FAN L M, DENG X W . Global genome expression analysis of rice in response to drought and high-salinity stresses in shoot, flag leaf, and panicle. Plant Molecular Biology, 2007,63(5):591-608.
[47]	AMAKO K, CHEN G X, ASADA K . Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants. Plant & Cell Physiology, 1994,35(3):497-504.
[48]	FOYER C H, NOCTOR G . Tansley review No. 112. Oxygen processing in photosynthesis: regulation and signalling. New Phytologist, 2000,146(3):359-388.
[49]	付晴晴, 谭雅中, 翟衡, 杜远鹏 . NaCl胁迫对耐盐性不同葡萄株系叶片活性氧代谢及清除系统的影响. 园艺学报, 2018,45(1):30-40.
	FU Q Q, TAN Y Z, ZHAI H, DU Y P . Effects of salt stress on the generation and scavenging of reactive oxygen species in leaves of grape strains with different salt tolerance. Acta Horticulturae Sinica, 2018,45(1):30-40. (in Chinese)

指标 Index	发芽势 GE	发芽率 GP	发芽指数 GI	芽长 SL	根长 RL	芽鲜重 SFW	根鲜重 RFW	活力指数 VI	盐害率 SDR
发芽势GE	1
发芽率GP	0.625**	1
发芽指数GI	0.748**	0.910**	1
芽长SL	0.310**	0.308**	0.427**	1
根长RL	0.029	0.143	0.150	0.377**	1
芽鲜重SFW	0.125	0.223*	0.213*	0.331**	0.117	1
根鲜重RFW	0.198*	0.275**	0.319**	0.464**	0.564**	0.360**	1
活力指数VI	0.290**	0.387**	0.445**	0.491**	0.543**	0.369**	0.982**	1
盐害率SDR	-0.759**	-0.916**	-0.996**	-0.411**	-0.144	-0.202*	-0.303**	-0.425**	1

主成分Principal component	特征值Eigen value	贡献率Contribution (%)	累计贡献率Cumulative contribution (%)
Ⅰ	4.399	49.980	49.980
Ⅱ	2.068	22.310	72.290
Ⅲ	1.030	9.900	82.190

主成分Principal component	相对发芽势 Relative germination energy	相对发芽率 Relative germination percentage	相对发芽指数Relative germination index	相对芽长Relative sprout length	相对根长Relative root length	相对芽鲜重 Relative shoot fresh weight	相对根鲜重Relative root fresh weigh	相对活力指数Relative vitality index	相对盐害率Relative salt damage rate
Ⅰ	0.704	0.824	0.893	0.626	0.419	0.405	0.666	0.753	-0.886
Ⅱ	-0.455	-0.413	-0.410	0.282	0.612	0.283	0.657	0.559	0.430
Ⅲ	-0.040	-0.030	-0.045	0.132	-0.413	0.827	-0.074	-0.073	0.052

主成分 Principal component	发芽势 GE	发芽率 GP	发芽指数 GI	芽长 SL	根长 RL	芽鲜重 SFW	根鲜重 RFW	活力指数 VI	盐害率 SDR
Ⅰ	0.157	0.183	0.198	0.139	0.093	0.090	0.148	0.167	-0.197
Ⅱ	-0.226	-0.205	-0.204	0.140	0.305	0.141	0.327	0.278	0.214
Ⅲ	-0.045	-0.034	-0.051	0.148	-0.464	0.929	-0.083	-0.082	0.059

编号Code	品种资源Germplasm	来源Source	F值F-value	排序Order
18	伊选大红糜 Yixuandahongmi	中国内蒙古Inner Mongolia, China	269.72	1
27	污咀黍 Wujushu	中国山西Shanxi, China	268.97	2
33	白黍 Baishu	中国山西Shanxi, China	264.54	3
45	竹叶青黄硬糜 Zhuyeqinghuangyingmi	中国甘肃Gansu, China	221.35	4
41	小黄糜子 Xiaohuangmizi	中国宁夏Ningxia, China	205.25	5
69	太原1036 Taiyuan 1036	中国黑龙江Heilongjiang, China	196.97	6
17	达旗青糜子 Daqiqingmizi	中国内蒙古Inner Mongolia, China	174.28	7
93	宁糜8号 Ningmi 8	中国宁夏Ningxia, China	165.39	8
6	蚂蚱眼 Mazhayan	中国辽宁Liaoning, China	157.45	9
86	外引黍8号 Waiyinshu 8	美国America	154.87	10
46	大黄粘糜子 Dahuangnianmizi	中国甘肃Gansu, China	146.75	11

糜子资源耐盐性评价与盐胁迫生理响应

Salt-Tolerance Evaluation and Physiological Response of Salt Stress of Broomcorn Millet (Panicum miliaceum L.)

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 49

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	王君杰,田翔,秦慧彬,王海岗,曹晓宁,陈凌,刘思辰,乔治军. 光周期对糜子生长发育及叶片内源激素的调控效应[J]. 中国农业科学, 2021, 54(2): 286-295.
[2]	董明,降彦苗,李海权,耿玲玲,刘建烨,乔志红,刘国庆. 光周期变化对糜子形态建成及幼穗发育进程的影响[J]. 中国农业科学, 2020, 53(6): 1118-1125.
[3]	王君杰,王海岗,曹晓宁,陈凌,刘思辰,田翔,秦慧彬,乔治军. 糜子不同性状光周期敏感性的综合评价[J]. 中国农业科学, 2020, 53(3): 474-485.
[4]	陈凌,王君杰,王海岗,曹晓宁,刘思辰,田翔,秦慧彬,乔治军. 耐低氮糜子品种的筛选及农艺性状的综合评价[J]. 中国农业科学, 2020, 53(16): 3214-3224.
[5]	陈小红,何杰丽,石甜甜,邵欢欢,王海岗,陈凌,高志军,王瑞云,乔治军. 基于转录组测序开发糜子SSR标记[J]. 中国农业科学, 2020, 53(10): 1940-1949.
[6]	寇淑君, 霍阿红, 付国庆, 纪军建, 王瑶, 左振兴, 刘敏轩, 陆平. 利用荧光SSR分析中国糜子的遗传多样性和群体遗传结构[J]. 中国农业科学, 2019, 52(9): 1475-1477.
[7]	石甜甜, 何杰丽, 高志军, 陈凌, 王海岗, 乔治军, 王瑞云. 利用EST-SSR评估糜子资源遗传差异[J]. 中国农业科学, 2019, 52(22): 4100-4109.
[8]	宫香伟,党科,李境,罗艳,赵冠,杨璞,高小丽,高金锋,王鹏科,冯佰利. 糜子绿豆间作模式下糜子光合物质生产及水分利用效率[J]. 中国农业科学, 2019, 52(22): 4139-4153.
[9]	罗艳, 屈洋, 杨清华, 张伟丽, 宫香伟, 李境, 高小丽, 高金锋, 杨璞, 王鹏科, 冯佰利. 播期对糜子农艺性状及淀粉理化性质的影响[J]. 中国农业科学, 2019, 52(22): 4154-4165.
[10]	王君杰,陈凌,王海岗,曹晓宁,刘思辰,田翔,秦慧彬,乔治军. 糜子叶片氮含量和籽粒蛋白质含量高光谱监测研究[J]. 中国农业科学, 2019, 52(15): 2593-2603.
[11]	宫香伟，韩浩坤，张大众，李境，王孟，薛志和，杨璞，高小丽，冯佰利. 氮肥运筹对糜子生育后期干物质积累与转运及叶片氮素代谢的调控效应[J]. 中国农业科学, 2018, 51(6): 1045-1056.
[12]	卞凤娥，肖秋红，郝桂梅，孙永江，陆文利，杜远鹏，翟衡. 根施褪黑素对NaCl胁迫下葡萄内源褪黑素及叶绿素荧光特性的影响[J]. 中国农业科学, 2018, 51(5): 952-963.
[13]	薛延桃，陆平，乔治军，刘敏轩，王瑞云. 基于SSR标记的黍稷种质资源遗传多样性及亲缘关系研究 [J]. 中国农业科学, 2018, 51(15): 2846-2859.
[14]	董孔军，刘天鹏，何继红，任瑞玉，张磊，许岩，杨天育. 黄土高原半干旱区不同覆膜方式对土壤水热环境及糜子耗水特性的影响[J]. 中国农业科学, 2018, 51(12): 2274-2287.
[15]	刁现民，程汝宏. "谷子糜子育种"专题导读:十五年区试数据分析展示谷子糜子育种现状[J]. 中国农业科学, 2017, 50(23): 4469-4474.