中国农业科学 ›› 2019, Vol. 52 ›› Issue (14): 2450-2467.doi: 10.3864/j.issn.0578-1752.2019.14.006
刘波1,陈倩倩1,王阶平1,阮传清1,陈燕萍1,夏江平2,车建美1,陈峥1,潘志针1,文笑2,朱育菁1,张海峰1,郑雪芳1
收稿日期:
2019-03-19
接受日期:
2019-05-09
出版日期:
2019-07-16
发布日期:
2019-07-26
作者简介:
刘波(通信作者),E-mail: fzliubo@163.com
基金资助:
Bo LIU1,QianQian CHEN1,JiePing WANG1,ChuanQing RUAN1,YanPing CHEN1,JiangPing XIA2,JianMei CHE1,Zheng CHEN1,ZhiZhen PAN1,Xiao WEN2,YuJing ZHU1,HaiFeng ZHANG1,XueFang ZHENG1
Received:
2019-03-19
Accepted:
2019-05-09
Online:
2019-07-16
Published:
2019-07-26
摘要:
【目的】利用微生物发酵床作为发酵槽,猪粪氮素连续流加中温好氧发酵,将通过宏基因检测鉴定到的微生物组称为整合微生物组,生产高含菌量整合微生物组菌剂,作为植物病害生防菌剂。【方法】生产工艺:原料配制→发酵床发酵→猪粪氮素连续流加→好氧发酵控制→产品加工→产品包装等。生产技术:利用养猪使用1年以上的微生物发酵床,添加一层10 cm厚的30%豆饼粉+70%杏鲍菇菌糠垫料,每平方米1头猪作为猪粪氮素连续流加营养来源,每天翻耕1次,连续好氧发酵20 d后,取出上层20 cm的垫料,进入晾晒、粉碎、分筛、包装,加工成整合微生物组菌剂。【结果】整合微生物组菌剂产品技术指标:含水量29.74%,pH 7.56,有机质含量44.46%,全氮含量2.23%,腐殖酸含量11.20%,粗纤维含量14.06%,含菌量145×10 8cfu/g。宏基因组测定结果表明,菌剂样品序列(reads)条数平均值为99 701.75,每克菌剂含有细菌39门、96纲、189目、383科、786属、1 281种;其中,芽孢杆菌46种,9种为中国新记录种,即:①嗜气芽孢杆菌(Bacillus aerophilus)、②蚯蚓芽孢杆菌(Bacillus eiseniae)、③丝状芽孢杆菌(Bacillus filamentosus)、④柯赫芽孢杆菌(Bacillus kochii)、⑤根际芽孢杆菌(Bacillus rhizosphaerae)、⑥长型赖氨酸芽孢杆菌(Lysinibacillus macroides)、⑦淤泥大洋芽孢杆菌(Oceanobacillus caeni)、⑧拾蛤鸟氨酸芽孢杆菌(Ornithinibacillus scapharcae)、⑨海洋枝芽孢杆菌(Virgibacillus oceani);未发现猪细菌病原。可培养法分离的芽孢杆菌活菌数2.062×10 8cfu/g,宏基因组测定结果中,芽孢杆菌的总丰度为1.42%,以此推算菌剂有效细菌总含量145×10 8cfu/g。整合微生物组菌剂浸出液处理组的绿豆发芽率为96.67%,与清水对照组无显著差异(P>0.05),但胚根长比对照增加了58.08%。用5%—10%的菌剂配制成育苗基质,番茄出苗率提高了3.0%,株高增加了25.1%,对番茄青枯病的校正防治效果可达79.41%。整合微生物组菌剂的产品质量标准参考农业农村部生物有机肥标准(NY884-2012),初步确定为:有机质≥40%,含水量≤30%,pH 5.5—7.5,粪大肠菌群数≤100个/g,蛔虫卵死亡率>95%,有效期>6个月;重金属含量满足标准要求:砷<15 mg·kg -1,镉≤15 mg·kg -1,铅≤15 mg·kg -1,铬≤15 mg·kg -1,汞≤15 mg·kg -1;有效活菌数调整为:总细菌数≥30×10 8cfu/g,其中芽孢杆菌≥2×10 8cfu/g。 【结论】提出了整合微生物组菌剂的概念和产品技术标准。研发的整合微生物菌剂可促进种子根部生长,并对番茄青枯病有良好的防治效果。
刘波,陈倩倩,王阶平,阮传清,陈燕萍,夏江平,车建美,陈峥,潘志针,文笑,朱育菁,张海峰,郑雪芳. 整合微生物组菌剂的提出、研发与应用[J]. 中国农业科学, 2019, 52(14): 2450-2467.
Bo LIU,QianQian CHEN,JiePing WANG,ChuanQing RUAN,YanPing CHEN,JiangPing XIA,JianMei CHE,Zheng CHEN,ZhiZhen PAN,Xiao WEN,YuJing ZHU,HaiFeng ZHANG,XueFang ZHENG. Proposition, Development and Application of the Integrated Microbiome Agent (IMA)[J]. Scientia Agricultura Sinica, 2019, 52(14): 2450-2467.
表1
整合微生物菌组剂营养成分分析"
样品序号 Sample number | 水分含量 Water content (%) | pH | 有机质 Organic material (%) | 全氮 Total nitrogen (%) | 腐殖酸 Humic acid (%) | 粗纤维 Coarse fiber (%) |
---|---|---|---|---|---|---|
g1 | 31.60 | 9.20 | 45.30 | 2.42 | 11.10 | 11.40 |
g2 | 27.60 | 7.10 | 38.90 | 2.31 | 9.31 | 11.30 |
g3 | 27.70 | 6.30 | 45.90 | 2.00 | 9.88 | 17.80 |
g4 | 33.60 | 6.20 | 45.10 | 2.10 | 11.40 | 14.80 |
g5 | 31.40 | 9.50 | 42.20 | 2.08 | 12.70 | 12.80 |
g6 | 28.70 | 8.10 | 45.30 | 2.62 | 12.90 | 13.00 |
g7 | 27.60 | 6.50 | 48.50 | 2.09 | 11.10 | 17.30 |
平均值Average | 29.74 | 7.56 | 44.46 | 2.23 | 11.20 | 14.06 |
表2
整合微生物组菌剂产品样品芽孢杆菌种类鉴定"
菌落编号 Colony number | 菌株编号 Strain number | 标准菌株学名 Name of Bacillus-like species | 16S rDNA相似度 16S rDNA similarity (%) |
---|---|---|---|
1. | FJAT-46225 | [1] 芽孢杆菌Bacillus sp. | 97.13 |
2. | FJAT-46301 | [2] 嗜气芽孢杆菌Bacillus aerophilus 28KT | 100.00 |
3. | FJAT-46217 | 100.00 | |
4. | FJAT-46253 | [3] 高地芽孢杆菌Bacillus altitudinis 41KF2bT | 100.00 |
5. | FJAT-46318 | [4] 解淀粉芽孢杆菌Bacillus amyloliquefaciens FZB42T | 99.72 |
6. | FJAT-46213 | [5] 蜡样芽孢杆菌Bacillus cereus ATCC 14579T | 99.70 |
7. | FJAT-46201 | 99.64 | |
8. | FJAT-46307 | [6] 阿氏芽孢杆菌Bacillus aryabhattai B8W22T | 100.00 |
9. | FJAT-46281 | 97.89 | |
10. | FJAT-46278 | 100.00 | |
11. | FJAT-46272 | 100.00 | |
12. | FJAT-46223 | 100.00 | |
13. | FJAT-46186 | 99.86 | |
14. | FJAT-46270 | [7] 克劳氏芽孢杆菌Bacillus clausii DSM 8716T | 99.50 |
15. | FJAT-46194 | 99.20 | |
菌落编号 Colony number | 菌株编号 Strain number | 标准菌株学名 Name of Bacillus-like species | 16S rDNA相似度 16S rDNA similarity (%) |
16. | FJAT-46263 | [8] 蚯蚓芽孢杆菌Bacillus eiseniae A1-2T | 99.58 |
17. | FJAT-46178 | 99.72 | |
18. | FJAT-46289 | [9] 丝状芽孢杆菌Bacillus filamentosus SGD-14T | 100.00 |
19. | FJAT-46204 | 100.00 | |
20. | FJAT-46297 | [10] 柯赫芽孢杆菌Bacillus kochii WCC 4582T | 99.93 |
21. | FJAT-46258 | 99.79 | |
22. | FJAT-46246 | 100.00 | |
23. | FJAT-46230 | 99.65 | |
24. | FJAT-46221 | 99.65 | |
25. | FJAT-46208 | 99.72 | |
26. | FJAT-46202 | 99.86 | |
27. | FJAT-46176 | 98.86 | |
28. | FJAT-46175 | 99.72 | |
29. | FJAT-46313 | [11] 地衣芽孢杆菌Bacillus licheniformis ATCC 14580T | 99.10 |
30. | FJAT-46312 | 99.30 | |
31. | FJAT-46296 | 99.70 | |
32. | FJAT-46271 | 99.37 | |
33. | FJAT-46268 | 99.23 | |
34. | FJAT-46266 | 99.37 | |
35. | FJAT-46260 | 99.40 | |
36. | FJAT-46244 | 99.72 | |
37. | FJAT-46239 | 99.30 | |
38. | FJAT-46237 | 97.70 | |
39. | FJAT-46235 | 99.16 | |
40. | FJAT-46233 | 99.37 | |
41. | FJAT-46218 | 98.30 | |
42. | FJAT-46215 | 99.65 | |
43. | FJAT-46212 | 97.50 | |
44. | FJAT-46203 | 99.93 | |
45. | FJAT-46200 | 99.72 | |
46. | FJAT-46196 | 99.79 | |
47. | FJAT-46188 | 99.60 | |
48. | FJAT-46182 | 99.70 | |
49. | FJAT-46180 | 99.60 | |
50. | FJAT-46173 | 99.23 | |
51. | FJAT-46197 | [12] 甲基营养型芽孢杆菌Bacillus methylotrophicus KACC 13105T | 100.00 |
52. | FJAT-46274 | [13] 根际芽孢杆菌Bacillus rhizosphaerae SC-N012T | 99.09 |
53. | FJAT-46174 | [14] 沙福芽孢杆菌Bacillus safensis FO-36bT | 100.00 |
54. | FJAT-46311 | [15] 索诺拉沙漠芽孢杆菌Bacillus sonorensis NBRC 101234T | 99.44 |
55. | FJAT-46255 | 99.30 | |
56. | FJAT-46199 | 99.37 | |
57. | FJAT-46177 | 99.44 | |
菌落编号 Colony number | 菌株编号 Strain number | 标准菌株学名 Name of Bacillus-like species | 16S rDNA相似度 16S rDNA similarity (%) |
58. | FJAT-46316 | [16] 枯草芽孢杆菌茵氏亚种Bacillus subtilis subsp. inaquosorum KCTC 13429T | 99.72 |
59. | FJAT-46298 | 99.93 | |
60. | FJAT-46295 | 99.93 | |
61. | FJAT-46279 | 99.93 | |
62. | FJAT-46259 | 99.93 | |
63. | FJAT-46252 | 99.79 | |
64. | FJAT-46251 | 99.72 | |
65. | FJAT-46248 | 99.93 | |
66. | FJAT-46247 | 99.93 | |
67. | FJAT-46242 | 99.79 | |
68. | FJAT-46238 | 99.93 | |
69. | FJAT-46231 | 99.93 | |
70. | FJAT-46229 | 99.93 | |
71. | FJAT-46220 | 99.37 | |
72. | FJAT-46211 | 99.93 | |
73. | FJAT-46206 | 99.93 | |
74. | FJAT-46185 | 99.79 | |
75. | FJAT-46184 | 99.93 | |
76. | FJAT-46262 | [17] 枯草芽孢杆菌枯草亚种Bacillus subtilis subsp. subtilis NCIB 3610T | 99.93 |
77. | FJAT-46241 | 99.93 | |
78. | FJAT-46234 | 99.93 | |
79. | FJAT-46219 | 99.93 | |
80. | FJAT-46214 | 99.93 | |
81. | FJAT-46190 | 99.93 | |
82. | FJAT-46179 | 99.93 | |
83. | FJAT-46314 | [18] 特基拉芽孢杆菌Bacillus tequilensis KCTC 13622T | 100.00 |
84. | FJAT-46257 | 100.00 | |
85. | FJAT-46250 | 99.79 | |
86. | FJAT-46216 | 100.00 | |
87. | FJAT-46191 | 99.86 | |
88. | FJAT-46249 | [19] 人参哈格瓦氏菌Bhargavaea ginsengige 14T | 99.79 |
89. | FJAT-46227 | 99.93 | |
90. | FJAT-46224 | 99.93 | |
91. | FJAT-46192 | 99.79 | |
92. | FJAT-46276 | [20] 赖氨酸芽孢杆菌Lysinibacillus sp. | 97.76 |
93. | FJAT-46275 | [21] 淤泥大洋芽孢杆菌Oceanobacillus caeni S-11T | 99.30 |
94. | FJAT-46254 | [22] 拾蛤鸟氨酸芽孢杆菌Ornithinibacillus scapharcae TW25T | 98.47 |
95. | FJAT-46303 | [23] 盐反硝化枝芽孢杆菌Virgibacillus halodenitrificans DSM10037T | 99.79 |
96. | FJAT-46195 | 99.86 | |
97. | FJAT-46309 | [24] 海洋枝芽孢杆菌Virgibacillus oceani MY11T | 98.70 |
98. | FJAT-46232 | 98.30 | |
99. | FJAT-46198 | 98.29 | |
100. | FJAT-46222 | [25] 盐湖枝芽孢杆菌Virgibacillus salinus XH-22T | 98.48 |
表3
整合微生物组菌剂芽孢杆菌活菌计数"
样本编号 Sample number | 芽孢杆菌活菌计数Colony forming unit of Bacillus species (×107cfu/g) | 平均值 Average (×107cfu/g) | ||
---|---|---|---|---|
重复I Rep1 | 重复II Rep2 | 重复III Rep3 | ||
g1 | 20 | 31 | 29 | 26.67 |
g2 | 23 | 18 | 22 | 21.00 |
g3 | 19 | 16 | 18 | 17.67 |
g4 | 26 | 18 | 21 | 21.67 |
g5 | 18 | 19 | 18 | 18.33 |
g6 | 21 | 15 | 19 | 18.33 |
g7 | 19 | 22 | 21 | 20.67 |
平均值 Average (×107cfu/g) | 20.85 | 19.85 | 21.14 | 20.62 |
表4
基于宏基因组分析整合菌剂细菌微生物组测定"
样本 Sample | 短序列 Reads | 种类 OTUs | 在0.97的相似度下Under the similarity of 0.97 | ||||
---|---|---|---|---|---|---|---|
Ace指数 Ace index | Chao指数 Chao index | 测序深度指数 Coverage index | 香农多样性指数 Shannon index | 辛普森优势度指数 Simpson index | |||
g1 | 102575 | 1608 | 1824 (1779, 1882) | 1879 (1809, 1973) | 0.997114 | 5.14 (5.13, 5.15) | 0.0212 (0.0208, 0.0216) |
g2 | 129891 | 1698 | 1922 (1875, 1981) | 1973 (1902, 2068) | 0.997721 | 5.55 (5.54, 5.55) | 0.0101 (0.01, 0.0103) |
g3 | 83279 | 1255 | 1604 (1536, 1689) | 1592 (1511, 1700) | 0.995845 | 4.68 (4.67, 4.70) | 0.0252 (0.0248, 0.0255) |
g4 | 137195 | 1192 | 1381 (1337, 1438) | 1421 (1356, 1512) | 0.998287 | 4.85 (4.85, 4.86) | 0.0187 (0.0185, 0.0189) |
g5 | 119811 | 1711 | 1997 (1942, 2067) | 2047 (1966, 2153) | 0.997087 | 5.11 (5.10, 5.12) | 0.0255 (0.0251, 0.026) |
g6 | 111290 | 1257 | 1566 (1503, 1646) | 1574 (1493, 1683) | 0.997232 | 4.96 (4.95, 4.97) | 0.0153 (0.0151, 0.0155) |
g7 | 113573 | 1564 | 1791 (1744, 1851) | 1817 (1752, 1904) | 0.997385 | 5.19 (5.17, 5.20) | 0.0159 (0.0157, 0.0161) |
平均值Average | 99701.75 | 1469.29 | 1726.42 | 1757.57 | 0.997239 | 5.0686 | 0.0188 |
表5
整合微生物组菌剂产品中芽孢杆菌种类丰度"
种名 Species name | 丰度Species richness (%) | 平均值 Average (%) | ||||||
---|---|---|---|---|---|---|---|---|
g1 | g2 | g3 | g4 | g5 | g6 | g7 | ||
[1] 穿琼脂氨芽孢杆菌 Ammoniibacillus agariperforans | 0.0000 | 0.0015 | 0.0000 | 0.0000 | 0.0000 | 0.0162 | 0.0000 | 0.0025 |
[2] 兼性芽孢杆菌Amphibacillus sp. | 0.0000 | 0.0000 | 0.0060 | 0.0160 | 0.0000 | 0.0036 | 0.0018 | 0.0039 |
[3] 解硫胺素芽孢杆菌Aneurinibacillus sp. | 0.0000 | 0.0008 | 0.0048 | 0.0000 | 0.0000 | 0.0009 | 0.0000 | 0.0009 |
[4] 产氮芽孢杆菌Bacillus azotoformans | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 |
[5] 草坪芽孢杆菌 Bacillus graminis | 0.0010 | 0.0023 | 0.0036 | 0.0015 | 0.0008 | 0.0018 | 0.0000 | 0.0016 |
[6] 土地芽孢杆菌Bacillus humi | 0.0010 | 0.0008 | 0.0084 | 0.0015 | 0.0025 | 0.0234 | 0.0009 | 0.0055 |
[7] 深层芽孢杆菌 Bacillus infernus | 0.0039 | 0.0054 | 0.0144 | 0.0036 | 0.0025 | 0.0036 | 0.0343 | 0.0097 |
[8] 芽孢杆菌1 Bacillus sp.1 | 0.0000 | 0.0108 | 0.0000 | 0.0000 | 0.0659 | 0.0009 | 0.0009 | 0.0112 |
[9] 芽孢杆菌2 Bacillus sp.2 | 0.0614 | 0.2079 | 1.2836 | 0.4665 | 0.1327 | 0.7332 | 0.4033 | 0.4698 |
[10] 热乳芽孢杆菌Bacillus thermolactis | 0.0010 | 0.0062 | 0.0120 | 0.0036 | 0.0025 | 0.0108 | 0.0158 | 0.0074 |
[11] 热碱芽孢杆菌CaldalkaLibacillus sp. | 0.0000 | 0.0015 | 0.0000 | 0.0000 | 0.0008 | 0.0099 | 0.0018 | 0.0020 |
[12] 脱硫芽孢杆菌 Desulfuribacillus sp. | 0.0088 | 0.0000 | 0.0000 | 0.0000 | 0.0008 | 0.0000 | 0.0000 | 0.0014 |
[13] 地芽孢杆菌1 Geobacillus sp.1 | 0.0000 | 0.0054 | 0.0012 | 0.0000 | 0.0017 | 0.3603 | 0.0018 | 0.0529 |
[14] 嗜热嗜脂肪地芽孢杆菌Geobacillus stearothermophilus | 0.0000 | 0.0015 | 0.0012 | 0.0029 | 0.0017 | 0.0018 | 0.0009 | 0.0014 |
[15] 纤细芽孢杆菌Gracilibacillus sp. | 0.0029 | 0.0100 | 0.0036 | 0.0058 | 0.0017 | 0.3423 | 0.0326 | 0.0570 |
[16] 喜盐芽孢杆菌Halobacillus sp. | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 |
[17] 嗜盐盐乳芽孢杆菌 Halolactibacillus halophilus | 0.0556 | 0.0031 | 0.0108 | 0.1844 | 0.0225 | 0.0117 | 0.0123 | 0.0429 |
[18] 盐乳芽孢杆菌Halothiobacillus sp. | 0.0010 | 0.0000 | 0.0012 | 0.0146 | 0.0000 | 0.0018 | 0.0044 | 0.0033 |
[19] 食淀粉乳杆菌Lactobacillus amylovorus | 0.1950 | 0.0254 | 0.0588 | 0.0722 | 0.0250 | 1.0145 | 0.1101 | 0.2144 |
[20] 动物乳杆菌Lactobacillus animalis | 0.0010 | 0.0023 | 0.0000 | 0.0036 | 0.0000 | 0.0036 | 0.0000 | 0.0015 |
[21] 乳杆菌新种 Lactobacillus equicursoris | 0.0010 | 0.0062 | 0.0000 | 0.0007 | 0.0000 | 0.0584 | 0.0044 | 0.0101 |
[22] 粘膜乳杆菌Lactobacillus mucosae | 0.0000 | 0.0008 | 0.0000 | 0.0015 | 0.0000 | 0.0306 | 0.0000 | 0.0047 |
[23] 清酒乳杆菌Lactobacillus sakei | 0.0000 | 0.0008 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0001 |
[24] 乳杆菌1 Lactobacillus sp.1 | 0.3246 | 0.0477 | 0.1465 | 0.4497 | 0.0209 | 0.6676 | 0.1330 | 0.2557 |
[25] 小鳟鱼大洋芽孢杆菌Oceanobacillus oncorhynchi | 0.0000 | 0.0008 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0001 |
[26] 大洋芽孢杆菌1 Oceanobacillus sp.1 | 0.0000 | 0.0000 | 0.0000 | 0.0066 | 0.0000 | 0.0045 | 0.0026 | 0.0020 |
[27] 大洋芽孢杆菌2 Oceanobacillus sp.2 | 0.0049 | 0.0023 | 0.0024 | 0.0117 | 0.0000 | 0.0521 | 0.0070 | 0.0115 |
[28] 加利福尼亚鸟氨酸芽孢杆菌 Ornithinibacillus californiensis | 0.0049 | 0.0154 | 0.0144 | 0.0117 | 0.0008 | 0.0279 | 0.0317 | 0.0152 |
[29] 鸟氨酸芽孢杆菌DX-3 Ornithinibacillus sp. DX-3 | 0.0019 | 0.0008 | 0.0060 | 0.0022 | 0.0025 | 0.0350 | 0.0352 | 0.0120 |
[30] 鸟氨酸芽孢杆菌GD05 Ornithinibacillus sp. GD05 | 0.0000 | 0.0008 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0026 | 0.0005 |
[31] 鸟氨酸芽孢杆菌HME7715 Ornithinibacillus sp. HME7715 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0180 | 0.0000 | 0.0026 |
[32] 类芽孢杆菌1 Paenibacillus sp.1 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0062 | 0.0009 |
[33] 类芽孢杆菌2 Paenibacillus sp.2 | 0.0078 | 0.0331 | 0.0096 | 0.0204 | 0.0150 | 0.0494 | 0.0819 | 0.0310 |
[34] 少盐芽孢杆菌1 Paucisalibacillus sp.1 | 0.0000 | 0.0000 | 0.0024 | 0.0000 | 0.0008 | 0.0009 | 0.0009 | 0.0007 |
[35] 少盐芽孢杆菌2 Paucisalibacillus sp.2 | 0.0029 | 0.0115 | 0.0060 | 0.0087 | 0.0025 | 0.0099 | 0.0123 | 0.0077 |
种名 Species name | 丰度Species richness (%) | 平均值 Average (%) | ||||||
g1 | g2 | g3 | g4 | g5 | g6 | g7 | ||
[36] 少盐芽孢杆菌3 Paucisalibacillus sp.3 | 0.0049 | 0.0000 | 0.0072 | 0.0095 | 0.0025 | 0.0584 | 0.0088 | 0.0130 |
[37] 厚胞鲁梅尔芽孢杆菌Rummeliibacillus pycnus | 0.0146 | 0.0062 | 0.0048 | 0.0073 | 0.0000 | 0.0045 | 0.0018 | 0.0056 |
[38] 森林土壤芽孢杆菌Solibacillus silvestris | 0.0049 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0009 | 0.0000 | 0.0008 |
[39] 硫化芽孢杆菌Sulfobacillus sp. | 0.0000 | 0.0023 | 0.0024 | 0.0000 | 0.0000 | 0.0000 | 0.0018 | 0.0009 |
[40] 热芽孢杆菌1 Thermobacillus sp.1 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0008 | 0.0000 | 0.0114 | 0.0018 |
[41] 热芽孢杆菌2 Thermobacillus sp.2 | 0.0000 | 0.0069 | 0.0024 | 0.0000 | 0.0008 | 0.0036 | 0.0018 | 0.0022 |
[42] 热芽孢杆菌3 Thermobacillus sp.3 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 |
[43] 热生肿块芽孢杆菌 Tuberibacillus calidus | 0.0000 | 0.0008 | 0.0024 | 0.0029 | 0.0000 | 0.0009 | 0.0106 | 0.0025 |
[44] 肿块芽孢杆菌Tuberibacillus sp. | 0.0000 | 0.0000 | 0.0216 | 0.8951 | 0.0000 | 0.0000 | 0.0379 | 0.1364 |
[45] 尿素芽孢杆菌Ureibacillus sp. | 0.0058 | 0.0023 | 0.0000 | 0.0000 | 0.0008 | 0.0054 | 0.0141 | 0.0041 |
[46] 火山芽孢杆菌Vulcanibacillus sp. | 0.0273 | 0.0008 | 0.0000 | 0.0153 | 0.0442 | 0.0027 | 0.0009 | 0.0130 |
总和Total | 0.7381 | 0.4244 | 1.6377 | 2.2195 | 0.3527 | 3.5710 | 1.0278 | 1.4244 |
表6
整合微生物组菌剂对绿豆发芽的影响"
处理 Treatment | 发芽率 Germination rate (%) | 胚根长Radical length (RL, cm) | 发芽指数Germination index (GI) | 活力指数Vigor index (VI) | |||
---|---|---|---|---|---|---|---|
RL | 比值Ratio | GI | 比值Ratio | VI | 比值Ratio | ||
整合菌剂IMA | 96.67±2.11a | 2.64±0.77A | 1.5808 | 57.67a | 0.9885 | 152.25±13.29A | 1.5626 |
清水对照CK | 98.33±1.53a | 1.67±0.23B | 1.0000 | 58.34a | 1.0000 | 97.43±10.67B | 1.0000 |
表7
整合微生物组菌剂对番茄穴盘育苗的影响"
处理组 Treatment | 处理编号 Treatment number | 整合菌剂含量IMA percent (%) | 番茄种苗生长状况Growth of tomato seedlings | |||
---|---|---|---|---|---|---|
出苗率 Emergence rate (%) | 根长 Root length (cm) | 株高 Plant height (cm) | 茎粗 Stem diameter (cm) | |||
高含量组 High content group | 处理1 Treatment 1 | 30 | 9.04 | 0.70 | 1.50 | 0.70 |
处理2 Treatment 2 | 25 | 46.10 | 1.45 | 6.23 | 1.05 | |
平均值Average | — | 27.57c | 1.08b | 3.87c | 0.88b | |
中含量组 Middle content group | 处理3 Treatment 3 | 20 | 85.30 | 4.45 | 9.78 | 1.56 |
处理4 Treatment 4 | 15 | 86.03 | 4.85 | 9.67 | 1.45 | |
平均值Average | — | 85.67b | 4.65a | 9.73b | 1.51a | |
低含量组 Low content group | 处理5 Treatment 5 | 10 | 89.50 | 4.73 | 10.67 | 1.49 |
处理6 Treatment 6 | 5 | 88.90 | 4.62 | 11.92 | 1.51 | |
平均值Average | — | 89.20a | 4.68a | 11.30a | 1.50a | |
空白对照CK | 对照 CK | 0 | 86.20b | 4.68a | 9.03b | 1.40a |
表8
整合微生物组菌剂对番茄种苗青枯病防控的影响"
处理组 Treatment | 处理编号 Treatment number | 整合菌剂含量 IMA percent (%) | 出苗30 d后接种青枯病原菌液 Inoculation of R. solanacearum 30 days after emergence (106 cfu/mL) | 10 d校正防效Corrected control efficacy of 10 d (%) | ||
---|---|---|---|---|---|---|
4 d后发病率 Incidence after 4 d (%) | 7 d后发病率 Incidence after 7 d (%) | 10 d后发病率 Incidence after 10 d (%) | ||||
高含量组 High content group | 处理1 Treatment 1 | 30 | — | — | — | |
处理2 Treatment 2 | 25 | 6.25 | 23.00 | 66.67 | ||
平均值Average | — | 6.25b | 23.00a | 66.67a | 17.88 | |
中含量组 Middle content group | 处理3 Treatment 3 | 20 | 6.47 | 13.33 | 32.40 | |
处理4 Treatment 4 | 15 | 8.33 | 16.57 | 28.50 | ||
平均值Average | — | 7.40ab | 14.95b | 30.45ab | 62.49 | |
低含量组 Low content group | 处理5 Treatment 5 | 10 | 6.67 | 14.47 | 18.30 | |
处理6 Treatment 6 | 5 | 6.67 | 13.33 | 15.14 | ||
平均值Average | — | 6.67b | 13.90b | 16.72b | 79.41 | |
空白对照CK | 对照 CK | 0 | 11.11a | 33.33a | 88.89a | — |
[1] | 曹怡, 王超群, 徐风, 贾秀虹, 刘广学, 杨生超, 龙光强, 陈中坚, 魏富刚, 杨绍周, 福田浩三, 王璇, 蔡少青 . 中药三七质量评价半微量方法及在三七连作障碍样品分析中的应用. 中国中药杂志, 2016,41(20):3773-3781. |
CAO Y, WANG C Q, XU F, JIA X H, LIU G X, YANG S C, LONG G Q, CHEN Z J, WEI F G, YANG S Z, FUKUDA K, WANG X, CAI S Q . A semimicroquality evaluation method on Panax notoginseng and its application in analysis of continuous cropping obstacles research samples. China Journal of Chinese Materia Medica, 2016,41(20):3773-3781. (in Chinese) | |
[2] |
QIN S, YEBOAH S, CAO L, ZHANG J, SHI S, LIU Y . Breaking continuous potato cropping with legumes improves soil microbial communities, enzyme activities and tuber yield. PLoS ONE, 2017,12(5):e0175934.
doi: 10.1371/journal.pone.0175934 |
[3] |
CHELLEMI D O, ROSSKOPF E N, KOKALIS-BURELLE N . The effect of transitional organic production practices on soilborne pests of tomato in a simulated microplot study. Phytopathology, 2013,103(8):792-801.
doi: 10.1094/PHYTO-09-12-0243-R |
[4] | 肖春萍, 杨利民, 马锋敏 . 栽培年限对人参根际土壤微生物活性及微生物量的影响. 中国中药杂志, 2014,39(24):4740-4747. |
XIAO C P, YANG L M, MA F M . Effects of growing time on Panax ginseng rhizosphere soil microbial activity and biomass. China Journal of Chinese Materia Medica, 2014,39(24):4740-4747. (in Chinese) | |
[5] |
LUAN F G, ZHANG L L, LOU Y Y, WANG L, LIU Y N. ZHANG H Y . Analysis of microbial diversity and niche in rhizosphere soil of healthy and diseased cotton at the flowering stage in southern Xinjiang. Genetics and Molecular Research, 2015,14(1):1602-1611.
doi: 10.4238/2015.March.6.7 |
[6] | 茹瑞红, 李烜桢, 黄晓书, 高峰, 王建明, 李本银, 张重义 . 食用菌菌渣缓解地黄连作障碍的研究. 中国中药杂志, 2014,39(16):3036-3041. |
RU R H, LI X Z, HUNAG X S, GAO F, WANG J M, LI B Y, ZHANG Z Y . Effect of substrate of edible mushroom on continuously cropping obstacle of Rehmannia glutinosa. China Journal of Chinese Materia Medica, 2014,39(16):3036-3041. (in Chinese) | |
[7] | 张重义, 李改玲, 牛苗苗, 范华敏, 李娟, 林文雄 . 连作地黄的生理生态响应与品质评价. 中国中药杂志, 2011,36(9):1133-1136. |
ZHANG Z Y, LI G L, NIU M M, FAN H M, LI J, LIN W X . Responses of physiological ecology and quality evaluation of Rehmannia gltinosa in continuous cropping. China Journal of Chinese Materia Medica, 2011,36(9):1133-1136. (in Chinese) | |
[8] | 尹文佳, 杜家方, 李娟, 张重义 . 连作对地黄生长的障碍效应及机制研究. 中国中药杂志, 2009,34(1):18-21. |
YIN W J, DU J F, LI J, ZHANG Z Y . Effects of continuous cropping obstacle on growth of Rehmannia glutinosa. China Journal of Chinese Materia Medica, 2009,34(1):18-21. (in Chinese) | |
[9] | 吴凤芝, 李敏, 曹鹏, 马亚飞, 王丽丽 . 小麦根系分泌物对黄瓜生长及土壤真菌群落结构的影响. 应用生态学报, 2014,25(10):2861-2867. |
WU F Z, LI M, CAO P, MA Y F, WANG L L . Effects of wheat root exudates on cucumber growth and soil fungal community structure. Chinese Journal of Applied Ecology, 2014,25(10):2861-2867. (in Chinese) | |
[10] | 赵娟, 薛泉宏, 杜军志, 陈姣姣 . 两株镰孢菌的鉴定及其粗毒素对甜瓜幼苗的化感作用. 应用生态学报, 2013,24(1):142-148. |
ZHAO J, XUE Q H, DU J Z, CHEN J J . Identification of two Fusarium isolates and their crude toxin allelopathic effect on Cucumis melo seedlings. Chinese Journal of Applied Ecology, 2013,24(1):142-148. (in Chinese) | |
[11] | 陈可, 孙吉庆, 刘润进, 李敏 . 丛枝菌根真菌对西瓜嫁接苗生长和根系防御性酶活性的影响. 应用生态学报, 2013,24(1):135-141. |
CHEN K, SUN J Q, LIU R J, LI M . Effects of arbuscular mycorrhizal fungus on the seedling growth of grafted watermelon and the defensive enzyme activities in the seedling roots. Chinese Journal of Applied Ecology, 2013,24(1):135-141. (in Chinese) | |
[12] | 孟品品, 刘星, 邱慧珍, 张文明, 张春红, 王蒂, 张俊莲, 沈其荣 . 连作马铃薯根际土壤真菌种群结构及其生物效应. 应用生态学报, 2012,23(11):3079-3086. |
MENG P P, LIU X, QIU H Z, ZHANG W M, ZHANG C H, WANG D, ZHANG J L, SHEN Q R . Fungal population structure and its biological effect in rhizosphere soil of continuously cropped potato. Chinese Journal of Applied Ecology, 2012,23(11):3079-3086. (in Chinese) | |
[13] | 张新慧, 张恩和, 王惠珍, 郎多勇 . 连作对当归生长的障碍效应及机制研究. 中国中药杂志, 2010,35(10):1231-1234. |
ZHANG X H, ZHANG E H, WANG H Z, LANG D Y . Effects of continuous cropping obstacle on growth of Angelica sinensis and its mechanism. China Journal of Chinese Materia Medica, 2010,35(10):1231-1234. (in Chinese) | |
[14] |
ZHANG X, LI X, ZHANG C, LI X, ZHANG H . Ecological risk of long-term chlorimuron-ethyl application to soil microbial community: an in situ investigation in a continuously cropped soybean field in Northeast China. Environmental Science and Pollution Research, 2011,18(3):407-415.
doi: 10.1007/s11356-010-0381-4 |
[15] | 银福军, 瞿显友, 曾纬, 舒抒 . 黄连不同部位水浸液自毒作用研究. 中药材, 2009,32(3):329-330. |
YIN F J, QU X Y, ZENG W, SHU S . Study on the autointoxication of aquatic extracts from different parts of Coptis chinensis. Journal of Chinese Medicinal Materials, 2009,32(3):329-330. (in Chinese) | |
[16] | 张淑香, 高子勤 . 连作障碍与根际微生态研究Ⅱ. 根系分泌物与酚酸物质. 应用生态学报, 2000,11(1):152-156. |
ZHANG S X, GAO Z Q . Continuous cropping obstacle and rhizospheric microecology. II. Root exudates and phenolic acids. Chinese Journal of Applied Ecology, 2000,11(1):152-156. (in Chinese) | |
[17] | LI T, LIU T, ZHENG C, KANG C, YANG Z, YAO X, SONG F, ZHANG R, WANG X, XU N, ZHANG C, LI W, LI S . Changes in soil bacterial community structure as a result of incorporation of Brassica plants compared with continuous planting eggplant and chemical disinfection in greenhouses. PLoS ONE, 2017,12(3):e0173923. |
[18] |
YANG H, LI J, XIAO Y, GU Y, LIU H, LIANG Y, LIU X, HU J, MENG D, YIN H . An integrated insight into the relationship between soil microbial community and tobacco bacterial wilt disease. Frontiers in Microbiology, 2017,8:2179.
doi: 10.3389/fmicb.2017.02179 |
[19] | NOVELLO G, GAMALERO E, BONA E, BOATTI L, MIGNONE F, MASSA N, CESARO P, LINGUA G, BERTA G . The rhizosphere bacterial microbiota of Vitis vinifera cv. Pinot Noir in an integrated pest management vineyard. Frontiers in Microbiology, 2017,8:1528. |
[20] |
KALIVAS A, GANOPOULOS I, PSOMOPOULOS F, GRIGORIADIS I, XANTHOPOULOU A, HATZIGIANNAKIS E, OSATHANUNKUL M, TSAFTARIS A, MADESIS P . Comparative metagenomics reveals alterations in the soil bacterial community driven by N-fertilizer and amino 16® application in lettuce. Genomics Data, 2017,14:14-17.
doi: 10.1016/j.gdata.2017.07.013 |
[21] | SHIVAJI S, CHATURVEDI P, SURESH K, REDDY G S, DUTT C B, WAINWRIGHT M, NARLIKAR J V, BHARGAVA P M . Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. International Journal of Systematic and Evolutionary Microbiology, 2006,56(7):1465-1473. |
[22] | HONG S W, PARK J M, KIM S J, CHUNG K S . Bacillus eiseniae sp. nov., a swarming, moderately halotolerant bacterium isolated from the intestinal tract of an earthworm( Eisenia fetida L.). International Journal of Systematic and Evolutionary Microbiology, 2012,62(9):2077-2083. |
[23] | SONALKAR V V, MAWLANKAR R, VENKATA RAMANA V, JOSEPH N, SHOUCHE Y S, DASTAGER S G . Bacillus filamentosus sp. nov., isolated from sediment sample. Antonie Van Leeuwenhoek, 2015,107(2):433-441. |
[24] | SEILER H, SCHMIDT V, WENNING M, SCHERER S . Bacillus kochii sp. nov., isolated from foods and a pharmaceuticals manufacturing site. International Journal of Systematic and Evolutionary Microbiology, 2012,62(5):1092-1097. |
[25] | MADHAIYAN M, POONGUZHALI S, LEE J S, LEE K C, HARI K . Bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil. Antonie Van Leeuwenhoek, 2011,100(3):437-444. |
[26] | COOREVITS A, DINSDALE A E, HEYRMAN J, SCHUMANN P, VAN LANDSCHOOT A, LOGAN NA, DE VOS P . Lysinibacillus macroides sp. nov., nom. rev. International Journal of Systematic and Evolutionary Microbiology, 2012,62(5):1121-1127. |
[27] | NAM J H, BAE W, LEE D H . Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea. International Journal of Systematic and Evolutionary Microbiology, 2008,58(5):1109-1113. |
[28] | SHIN N R, WHON T W, KIM M S, ROH S W, JUNG M J, KIM Y O, BAE J W . Ornithinibacillus scapharcae sp. nov., isolated from a dead ark clam. Antonie Van Leeuwenhoek, 2012,101(1):147-154. |
[29] | YIN X, YANG Y, WANG S, ZHANG G . Virgibacillus oceani sp. nov. isolated from ocean sediment. International Journal of Systematic and Evolutionary Microbiology, 2015,65(1):159-164. |
[30] | CARRASCO I J, MÁRQUEZ M C, VENTOSA A . Virgibacillus salinus sp. nov., a moderately halophilic bacterium from sediment of a saline lake. International Journal of Systematic and Evolutionary Microbiology, 2009,59(12):3068-3073. |
[31] | CHEN Y G, GU F L, LI J H, XU F, HE S Z, FANG Y M . Bacillus vanillea sp. nov., isolated from the cured vanilla bean. Current Microbiology, 2015,70(2):235-239. |
[32] |
HOOPER L V, GORDON J I . Commensal host-bacterial relationships in the gut. Science, 2001,292(5519):1115-1118.
doi: 10.1126/science.1058709 |
[33] |
KALLMEYER J, POCKALNY R, ADHIKARI R R, SMITH D C, D’HONDT S . Global distribution of microbial abundance and biomass in subseafloor sediment. Proceedings of the National Academy of Sciences of the United States of America, 2012,109(40):16213-16216.
doi: 10.1073/pnas.1203849109 |
[34] |
MENDES R, GARBEVA P, RAAIJMAKERS J M . The rhizosphere microbiome: significance of plant beneficial, plant pathogenic, and human pathogenic microorganism. FEMS Microbiology Reviews, 2013,37(5):634-663.
doi: 10.1111/1574-6976.12028 |
[35] | 李静, 李荣, 沈其荣, 俞萍, 余光辉 . 添加动物源氨基酸水解液研制生物有机肥. 环境科学研究, 2017,30(6):967-973. |
LI J, LI R, SHEN Q R, YU P, YU G H . Development of bio-organic fertilizer by adding amino acids hydrolyzed from animal carcasses. Research of Environmental Sciences, 2017,30(6):967-973. (in Chinese) | |
[36] | 陈智毅, 赵晓丽, 刘学铭 . 金针菇菌糠堆肥生产有机肥研究. 中国食用菌, 2012,31(4):30-31. |
CHEN Z Y, ZHAO X L, LIU X M . Study on composting of organic fertilizer produced by spent mushroom substrate from Flammulina velutipes. Edible Fungi of China, 2012,31(4):30-31. (in Chinese) | |
[37] | 张志红, 李华兴, 冯宏, 赵兰凤, 李敏清, 胡伟 . 堆肥作为微生物菌剂载体的研究. 农业环境科学学报, 2010,29(7):1382-1387. |
ZHANG Z H, LI H X, FENG H, ZHAO L F, LI M Q, HU W . Compost as a carrier for microbial inoculants. Journal of Agro-Environment Science, 2010,29(7):1382-1387. (in Chinese) | |
[38] | 李季 . 堆肥发酵接种剂及规模化生物有机肥产业化. 中国科技奖励, 2008(10):21. |
LI J . Composting fermentation inoculant and industrialization of large-scale bio-organic fertilizer. China Awards for Science and Technology, 2008(10):21. (in Chinese) | |
[39] | 张发宝, 徐培智, 唐拴虎, 陈建生, 谢开治, 黄旭 . 畜禽粪好氧堆肥产品的理化性质及生物效应. 广东农业科学, 2008(5):54-57. |
ZHANG F B, XU P Z, TANG S H, CHEN J S, XIE K Z, HUANG X . Physicochemical properties and biological effects of aerobically composted livestock manure products.Guangdong Agricultural Sciences, 2008(5):54-57. (in Chinese) | |
[40] | 田旸, 柳丽芬, 张兴文, 杨凤林, 谭伟杰 . 秸秆与污泥混合堆肥研究. 大连理工大学学报, 2003,43(6):753-758. |
TIAN Y, LIU L F, ZHANG X W, YANG F L, TAN W J . Study of co-composting of sewage sludge and straw biomass. Journal of Dalian University of Technology, 2003,43(6):753-758. (in Chinese) | |
[41] | 扎史品楚, 农传江, 王宇蕴, 徐智 . 生物有机肥的发酵工艺及应用效果研究. 环境工程, 2015,33(增刊):1011-1014, 1020. |
ZHASHIPC, NONG C J, WANG Y Y, XU Z . Research of microbial organic fertilizer fermentation process and its application. Environmental Engineering, 2015,33(Suppl.):1011-1014, 1020. (in Chinese) | |
[42] | 陈谦, 张新雄, 赵海, 官家发 . 生物有机肥中几种功能微生物的研究及应用概况. 应用与环境生物学报, 2010,16(2):294-300. |
CHEN Q, ZHANG X X, ZHAO H, GUAN J F . Advance in research and application of some functional microbes in bio-organic fertilizer. Chinese Journal of Applied and Environmental Biology, 2010,16(2):294-300. (in Chinese) | |
[43] | 张毅民, 万先凯 . 微生物菌群在生物有机肥制备中研究进展. 化学工业与工程, 2003,20(6):523-527. |
ZHANG Y M, WAN X K . Progress of microorganisms in bioorganic fertilizer production. Chemical Industry and Engineering, 2003,20(6):523-527. (in Chinese) | |
[44] | 陈倩倩, 刘波, 王阶平, 刘国红, 车建美, 陈峥, 唐建阳 . 微生物发酵床猪舍不同发酵等级垫料中大肠杆菌的分离鉴定. 中国畜牧兽医, 2017,44(1):268-274. |
CHEN Q Q, LIU B, WANG J P, LIU G H, CHE J M, CHEN Z, TANG J Y . Isolation and identification of E. coli in the microbial- fermentation bed of piggery. China Animal Husbandry and Veterinary Medicine, 2017,44(1):268-274. (in Chinese) | |
[45] | 陈星言, 邵镜颐, 吕建洲 . 龙血竭制剂对绿豆种子萌发和幼苗生长的影响. 园艺与种苗, 2016(7):86-88. |
CHEN X Y, SHAO J Y, LÜ J Z . Effect ofResina draconis on germination and seedling growth of mung bean. Horticulture and Seed, 2016(7):86-88. (in Chinese) | |
[46] | 俞家楠, 刘照斌, 吕建洲 . 肌苷对绿豆种子萌发和幼苗生长的影响. 中国农学通报, 2015,31(17):53-57. |
YU J N, LIU Z B, LÜ J Z . Effects of inosine on seed germination and seedling growth of mung bean. Chinese Agricultural Science Bulletin, 2015,31(17):53-57. (in Chinese) | |
[47] | 邢亚亮, 成月娇 . 青霉素对绿豆发芽及幼苗生长的影响. 农业技术与装备, 2013(7):66-68. |
XING Y L, CHENG Y J . Effects of penicillin on seed germination and seedling growth of mung bean.Agricultural Technology and Equipment, 2013(7):66-68. (in Chinese) | |
[48] | 高小宽, 魏淑珍 . 根际微生物对绿豆发芽率的影响. 江苏农业科学, 2010(1):106-108. |
GAO X K, WEI S Z . Effects of rhizospheric microorganism on seed germination of mung bean.Jiangsu Agricultural Sciences, 2010(1):106-108. (in Chinese) |
[1] | 逄洪波, 程露, 于茗兰, 陈强, 李玥莹, 吴隆坤, 王泽, 潘孝武, 郑晓明. 栽培稻芽期耐低温全基因组关联分析[J]. 中国农业科学, 2022, 55(21): 4091-4103. |
[2] | 路粉,孟润杰,吴杰,赵建江,李洋,毕秋艳,韩秀英,李敬华,王文桥. 马铃薯晚疫病菌对霜脲氰抗性动态监测及药效验证[J]. 中国农业科学, 2022, 55(18): 3556-3564. |
[3] | 周京龙,冯自力,魏锋,赵丽红,张亚林,周燚,冯鸿杰,朱荷琴. 棉花内生细菌YUPP-10及其分泌蛋白CGTase对棉花枯萎病的防治作用及机理[J]. 中国农业科学, 2021, 54(17): 3691-3701. |
[4] | 解昆仑,刘莉铭,刘美,彭斌,吴会杰,古勤生. 小西葫芦黄花叶病毒dsRNA的原核表达及其对ZYMV的防治效果[J]. 中国农业科学, 2020, 53(8): 1583-1593. |
[5] | 周华飞,杨红福,姚克兵,庄义庆,束兆林,陈志谊. FliZ调控枯草芽孢杆菌Bs916生物膜形成 及其对水稻纹枯病的防治效果[J]. 中国农业科学, 2020, 53(1): 55-64. |
[6] | 揣红运,石延霞,柴阿丽,杨杰,谢学文,李宝聚. 10%乙霉威·腐霉利微粉剂的研制及其 对黄瓜棒孢叶斑病的防治效果[J]. 中国农业科学, 2019, 52(6): 1009-1020. |
[7] | 赵天瑶,张亚宏,金涛,康玉凡. 绿豆萌发过程中蛋白组分及亚基变化[J]. 中国农业科学, 2018, 51(9): 1783-1794. |
[8] | 禾丽菲,陈乐乐,肖斌,赵时峰,李秀环,慕卫,刘峰. 番茄叶霉病菌对咯菌腈敏感基线的建立及田间防治效果评价[J]. 中国农业科学, 2018, 51(8): 1475-1483. |
[9] | 曹海潮,李秀环,王晓坤,白海秀,慕卫,刘峰. 吡唑醚菌酯及三唑类杀菌剂对番茄颈腐根腐病的防治效果[J]. 中国农业科学, 2018, 51(21): 4065-4075. |
[10] | 郑雪芳,刘波,朱育菁,陈德局,陈小强. 高效离子交换色谱法分析青枯雷尔氏菌Tn5转座子突变菌株的异质性[J]. 中国农业科学, 2018, 51(2): 268-278. |
[11] | 路粉,赵建江,刘晓芸,孟润杰,吴杰,韩秀英,王文桥. 马铃薯晚疫病菌对甲霜灵的抗性监测及替代药剂防治效果[J]. 中国农业科学, 2018, 51(14): 2700-2710. |
[12] | 黄学屏,宋昱菲,罗健,赵时峰,慕卫,刘峰. 蔬菜菌核病菌对氟吡菌酰胺的敏感性及防病应用潜力评估[J]. 中国农业科学, 2018, 51(14): 2711-2718. |
[13] | 顾中言,徐德进,徐广春. 田间药液用量影响农药单位剂量防治效果的原因分析[J]. 中国农业科学, 2018, 51(13): 2513-2523. |
[14] | 高杨杨,禾丽菲,李北兴,林琎,慕卫,刘峰. 山东省辣椒炭疽病病原菌的鉴定及高效防治药剂的筛选[J]. 中国农业科学, 2017, 50(8): 1452-1464. |
[15] | 蓝月,胡月,王琰,郭艳珍,赵恒科,何林,钱坤. 界面聚合制备乙草胺微胶囊及其杂草控制效果和环境残留[J]. 中国农业科学, 2017, 50(14): 2739-2747. |
|