大豆RN型细胞质雄性不育系雌性育性对异交率的影响

doi:10.3864/j.issn.0578-1752.2019.08.003

摘要/Abstract

摘要：

【目的】研究RN型大豆细胞质雄性不育系、保持系雌性育性差异,明确RN型大豆细胞质雄性不育系是否存在雌性育性降低的现象,探讨不育系雌性育性与异交率的相关性。【方法】首先在200余份RN型细胞质雄性不育系中,依据异交结实率高低,选择有代表性的不育系及保持系6对;然后通过网室内投放蜜蜂传粉对不育系进行异交率鉴定,确定不育系的异交率高低水平;再对6份不育系利用同一父本恢复系进行不去雄人工杂交试验,明确不同异交结实率不育系在接受外来花粉受精结实方面是否存在差异;最后利用同一恢复系作共同父本,通过去雄、不去雄人工平行杂交方法,研究不育系及对应保持系间杂交成活率差异,对6份不育系及6份对应保持系雌性育性进行分析,同时分析不育系异交率与雌性育性的相关性。【结果】网室异交率鉴定表明,供试6份不育系异交率存在显著差异,最高达49.46%,最低仅15.94%。6份不育系在人工授粉杂交成活率上也存在显著差异,高异交率不育系（JLCMS101A和JLCMS82A）杂交成活率显著高于中、低异交率不育系,中异交率不育系（JLCMS9A和JLCMS47A）杂交成活率显著高于低异交率不育系（JLCMS89A和JLCMS31A）。人工去雄平行杂交成活率,高、中异交率不育系显著高于低异交率不育系;高、中、低异交率保持系间杂交成活率无显著差异;杂交成活率在高、中异交率不育系与对应保持系间无显著差异,而低异交率不育系的杂交成活率显著低于其对应保持系的杂交成活率。人工不去雄平行杂交成活率,高异交率不育系杂交成活率显著高于中、低异交率不育系杂交成活率;高、中、低异交率保持系间杂交成活率无显著差异;高、中异交率不育系的杂交成活率与对应保持系的杂交成活率无显著差异,而低异交率不育系的杂交成活率则显著低于其对应保持系的杂交成活率。【结论】在大豆RN型细胞质雄性不育系中,高异交率不育系雌性育性正常,低异交率不育系中存在因雌性育性差而影响正常结实的情况,雌性育性差是造成其异交结实性低的原因之一,不同异交率不育系对应保持系雌性育性均正常;不育系网室异交率与不育系去雄杂交成活率呈极显著正相关,不育系网室异交率与不育系不去雄杂交成活率也呈极显著正相关。去雄和不去雄平行杂交结果均可用于鉴定不育系的雌性育性。

关键词: 大豆, 不育系, 异交率, 雌性育性

Abstract:

【Objective】To study the differences in female fertility of RN-type soybean cytoplasmic male sterile lines (A-lines) and maintainer lines (B-lines), determine whether there is a decline in female fertility in RN-type soybean cytoplasmic male sterility lines, and explore the correlation between female fertility and outcrossing rate in male sterile lines. 【Method】 Firstly, 6 pairs of sterile lines and maintainer lines were selected from more than 200 RN-type cytoplasmic male sterile lines; outcrossing rates among these lines were different. Second, the outcrossing rate of male sterile lines was evaluated using honeybee pollination in net cages. Third, six male sterile lines were pollinated by non-removing stamen test, with male parents of the same restorer line, to determine whether there were differences in fertilization and podding. Finally, the differences in survival rate between sterile lines and maintainer lines were studied by removing stamens and non-removing stamens parallel cross test with the same restorer line used as the male parent. 【Result】 The outcrossing rates in cages were significantly different among the six sterile lines tested: the highest outcrossing rate was 49.46% and the lowest outcrossing rate was 15.94%. there were significant differences among survival rates of artificial crosses of six sterile lines tested: the survival rate of sterile lines with a high outcrossing rate were significantly higher than that of medium and low outcrossing rate sterile lines and the survival rate of sterile lines with medium outcrossing rate were significantly higher than that of low outcrossing rate sterile lines. For crossing survival rate after artificially removing stamens, A-lines with high and medium outcrossing rates were significantly higher than that with low outcrossing rates; there were no significant differences among B-lines with high, medium, or low outcrossing rates; there were no significant differences among high A-lines, middle A-lines, and their corresponding B-lines, while A-lines with a low outcrossing rate were significantly lower than their corresponding B-lines. For crossing survival rate by artificially not removing stamen, A-lines with a high outcrossing rate were significantly higher than that with medium or low outcrossing rates; there were no significant differences among B-lines with high, medium and low outcrossing rates; there were no significant differences among high A-lines, middle A-lines, and their corresponding B-lines, while A-lines with low outcrossing rate were significantly lower than their corresponding B-lines. 【Conclusion】 For RN-type cytoplasmic male-sterile lines of soybean, female fertility of A-lines with high outcrossing rates were normal and female fertility of a few A-lines with low outcrossing rates were poor, affecting their seed setting. Female fertility of B-lines with different outcrossing rates were all normal, the outcrossing rate of A-lines in cages were significantly positively correlated with their crossing survival rate following artificial stamen removal and in plants where the stamen was not removed, the outcrossing rates of A-lines in cages were not correlated with their B-lines’ crossing survival rate. All parallel crosses by removing and non-removing stamens can thus be used to evaluate female fertility of A-lines.

Key words: soybean, Male-Sterile Lines, outcrossing rate, female fertility

张井勇,闫昊,彭宝,张春宝,李慧,王鹏年,丁孝羊,林春晶,孙寰,赵丽梅,张伟. 大豆RN型细胞质雄性不育系雌性育性对异交率的影响[J]. 中国农业科学, 2019, 52(8): 1324-1333.

ZHANG JingYong,YAN Hao,PENG Bao,ZHANG ChunBao,LI Hui,WANG PengNian,DING XiaoYang,LIN ChunJing,SUN Huan,ZHAO LiMei,ZHANG Wei. Effects of Female Fertility of RN Type Male-Sterile Lines on Outcrossing Rate[J]. Scientia Agricultura Sinica, 2019, 52(8): 1324-1333.

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参考文献 40

[1]	孙寰, 赵丽梅, 黄梅 . 大豆质核互作不育系研究. 科学通报, 1993,38(16):1535-1536.
	SUN H, ZHAO L M, HUANG M . Studies on cytoplasmic-nuclear male sterile soybean. Chinese Science Bulletin, 1993,38(16):1535-1536. (in Chinese)
[2]	孙寰, 赵丽梅, 黄梅 . 质核互作雄性不育大豆及生产大豆杂交种的方法: 中国,ZL97 112173.7. 1998-2-18.
	SUN H, ZHAO L M, HUANG M . Cytoplasmic-genetic male sterile soybean and method for producing hybrid soybean: China,ZL97 112173.7. 1998-2-18. (in Chinese)
[3]	SUN H, ZHAO L M, HUANG M . Cytoplasmic-genetic male sterile soybean and method for producing hybrid soybean: United State, No. US 6,320,098B1. 2001.
[4]	孙寰, 赵丽梅, 王曙明, 王跃强, 李建平 . 大豆杂种优势利用研究进展. 中国油料作物学报, 2003,25(1):92-96.
	SUN H, ZHAO L M, WANG S M, WANG Y Q, LI J P . Research progress on the use of heterosis in soybean. Chinese Journal of Oil Crop Sciences, 2003,25(1):92-96. (in Chinese)
[5]	赵丽梅, 孙寰, 王曙明, 王跃强, 黄梅, 李建平 . 大豆杂交种杂交豆1号选育报告. 中国油料作物学报, 2004,26(3):15-17.
	ZHAO L M, SUN H, WANG S M, WANG Y Q, HUANG M, LI J P . Breeding of hybrid soybean HybSoy 1. Chinese Journal of Oil Crop Sciences, 2004,26(3):15-17. (in Chinese)
[6]	张磊, 戴瓯和 . 大豆质核互作不育系W931A的选育研究. 中国农业科学, 1997,30(6):90-91.
	ZHANG L, DAI O H . Selection and breeding of nucleo-cytoplasmic male sterile line W931A in soybean. Scientia Agricultura Sinica, 1997,30(6):90-91. (in Chinese)
[7]	赵丽梅, 孙寰, 黄梅 . 大豆细胞质雄性不育系ZA的选育和初步研究. 大豆科学, 1998(3):268-270.
	ZHAO L M, SUN H, HUANG M . The development and preliminary studies on cytoplasmic male sterile soybean line ZA. Soybean Science, 1998(3):268-270. (in Chinese)
[8]	盖钧镒, 丁德荣, 崔章林, 邱家驯 . 大豆质核互作雄性不育系NJCMS1A的选育及其特性. 中国农业科学, 1999,32(5):23-27.
	GAI J Y, DING D R, CUI Z L, QIU J X . Development and performance of the cytoplasmic-nuclear male sterile line NJCMS1A of soybean. Scientia Agricultura Sinica, 1999,32(5):23-27. (in Chinese)
[9]	赵团结, 盖钧镒 . 大豆不育细胞质资源的发掘与鉴定. 作物学报, 2006,32(11):1604-1610.
	ZHAO T J, GAI J Y . Identification and evaluation of new sources of male-sterile cytoplasm in soybean. Acta Agronomica Sinica, 2006,32(11):1604-1610. (in Chinese)
[10]	NIE Z X, ZHAO T J, YANG S P, GAI J Y . Development of a cytoplasmic male-sterile line NJCMS4A for hybrid soybean production. Plant Breeding, 2017,136(4):516-525. doi: 10.1111/pbr.2017.136.issue-4
[11]	张磊, 戴瓯和, 黄志平, 李杰, 张丽亚, 胡晨 . 杂交大豆杂优豆1号选育. 大豆科技, 2007,25(2):14-16.
	ZHANG L, DAI O H, HUANG Z P, LI J, ZHANG L Y, HU C . Breeding of hybrid soybean Zayoudou No. 1. Soybean Science & Technology, 2007,25(2):14-16. (in Chinese)
[12]	彭宝, 赵丽梅, 王曙明, 程砚喜, 孙寰, 王跃强, 李建平, 张伟龙 . 杂交豆2号选育及高产制种技术研究. 吉林农业科学, 2008,33(2):3-4, 7.
	PENG B, ZHAO L M, WANG S M, CHENG Y X, SUN H, WANG Y Q, LI J P, ZHANG W L . Studies on breeding of HybSoy2 soybean and high yield seed production technique. Journal of Jilin Agricultural Sciences, 2008,33(2):3-4, 7. (in Chinese)
[13]	彭宝, 赵丽梅, 张伟龙, 张井勇, 孙寰, 王曙明, 李建平, 李茂海 . 大豆杂交种杂交豆3号选育报告. 吉林农业科学, 2010,35(6):4-5.
	PENG B, ZHAO L M, ZHANG W L, ZHANG J Y, SUN H, WANG S M, LI J P, LI M H . A breeding report of hybrid soybean HybSoy3. Journal of Jilin Agricultural Sciences, 2010,35(6):4-5. (in Chinese)
[14]	彭宝, 张连发, 张伟龙, 赵丽梅, 张春宝, 赵晓明, 张井勇, 孙寰, 徐亚杰, 路明鉴 . 大豆杂交种杂交豆5号选育报告. 吉林农业科学, 2011,36(6):7-8.
	PENG B, ZHANG L F, ZHAGN W L, ZHAO L M, ZHANG C B, ZHAO X M, ZHANG J Y, SUN H, XU Y J, LU M J . A breeding report of hybrid soybean HyBsoy 5. Journal of Jilin Agricultural Sciences, 2011,36(6):7-8. (in Chinese)
[15]	黄志平, 李杰坤, 张磊, 胡国玉, 胡晨, 张丽亚 . 高蛋白杂交大豆“杂优豆2号”选育及栽培技术. 安徽农业科学 , 2013,41(5): 2029,2133.
	HUANG Z P, LI J K, ZHANG L, HU G Y, HU C, ZHANG L Y . Study on breeding and cultivation technology of hybrid soybean Zayoudou No.2 with high-protein. Journal of Anhui Agriculture Science , 2013,41(5): 2029,2133. (in Chinese)
[16]	彭宝, 张伟龙, 张井勇, 张春宝, 闫昊, 张伟, 赵丽梅 . 杂交大豆新品种吉育607选育报告. 大豆科技, 2013(4):31-33.
	PENG B, ZHANG W L, ZHANG J Y, ZHAGN C B, YAN H, ZHANG W, ZHAO L M . A breeding report of hybrid soybean Jiyu 607. Soybean Science & Technology, 2013(4):31-33. (in Chinese)
[17]	彭宝, 张伟, 张春宝, 闫昊, 李宏来, 赵丽梅, 张伟龙, 张井勇 . 杂交大豆吉育606选育及高产制种技术研究. 作物杂志, 2013(5):159-160.
	PENG B, ZHANG W, ZHANG C B, YAN H, LI H L, ZHAO L M, ZHANG W L, ZHANG J Y . A breeding report and high yield seed production technique of hybrid soybean Jiyu606. Crops, 2013(5):159-160. (in Chinese)
[18]	李智, 周洪利 . 强优势阜杂交豆1号选育报告. 现代农业科技, 2014(12):43.
	LI Z, ZHOU H L . A breeding report of high heterosis FU hybrid soybean1. Xiandai Nongye Keji, 2014(12):43. (in Chinese)
[19]	卫保国, 卫一超, 白志元, 雷梦林, 张海平, 张瑞军 . 杂交大豆新品种晋豆48号的选育及制种技术. 中国种业, 2015(9):65-66.
	WEI B G, WEI Y C, BAI Z Y, LEI M L, ZHANG H P, ZHANG R J . A breeding report and yield seed production technique of hybrid soybean new variety Jindou48, China Seed Industry, 2015(9):65-66. (in Chinese)
[20]	彭宝, 张春宝, 严昊, 张井勇, 张伟龙, 赵丽梅, 张伟, 赵鑫 . 杂交大豆吉育609选育及栽培要点. 大豆科技, 2016(4):29-31.
	PENG B, ZHANG C B, YAN H, ZHANG J Y, ZHANG W L, ZHAO L M, ZHANG W, ZHAO X . A breeding report and cultivation technology of hybrid soybean Jiyu 609. Soybean Science & Technology, 2016(4):29-31. (in Chinese)
[21]	彭宝 . 杂交大豆新品种吉育612的选育//中国作物学会大豆专业委员会. 第十届全国大豆学术讨论会论文摘要集. 2017: 8, 74.
	PENG B . A breeding report of hybrid soybean Jiyu 612// China Crop Science Society Soybean Specialized Committee. Abstracts of Papers of 10th National Soybean Symposium. 2017: 8, 74. (in Chinese)
[22]	彭宝, 赵丽梅, 张井勇, 闫昊, 张春宝, 张伟, 张伟龙, 王鹏年, 丁孝羊 . 杂交大豆吉育610选育及栽培要点. 大豆科技, 2017(4):49-51.
	PENG B, ZHAO L M, ZHANG J Y, YAN H, ZHANG C B, ZHANG W, ZHANG W L, WANG P N, DING X Y . A breeding report and cultivation technology of hybrid soybean Jiyu 610. Soybean Science & Technology, 2017(4):49-51. (in Chinese)
[23]	赵丽梅, 彭宝, 张伟龙, 张连发, 张井勇, 李建平, 李茂海, 孙寰 . 杂交大豆制种技术体系的建立. 大豆科学, 2010,29(4):707-711.
	ZHAO L M, PENG B, ZHANG W L, ZHANG L F, ZHANG J Y, LI J P, LI M H, SUN H . Establishment of technology system for hybrid soybean seed production. Soybean Science, 2010,29(4):707-711. (in Chinese)
[24]	DAI J Y, ZHANG R J, WEI B G, NIE Z X, XING G N, ZHAO T J, YANG S P, GAI J Y . Key biological factors related to outcrossing- productivity of cytoplasmic-nuclear male-sterile lines in soybean [Glycine max,(L.) Merr.]. Euphytica, 2017,213(12):266.
[25]	盖钧镒, 代金英, 杨守萍, 张瑞军, 白志元, 卫一超, 卫保国, 张海平 . 一种提高大豆不育系结实性的方法: 中国,CN106472301A. 2017-03-08.
	GAI J Y, DAI J Y, YANG S P, ZHANG R J, BAI Z Y, WEI Y C, WEI B G, ZHANG H P . A method to improve seed setting of soybean sterile line: China,CN106472301A. 2017-03-08. (in Chinese)
[26]	张伟龙, 闫昊, 张春宝, 张井勇, 彭宝, 赵丽梅 . 密度与施氮量对大豆细胞质雄性不育系结实率及制种产量的影响. 吉林农业大学学报, 2016,38(6):671-674.
	ZHANG W L, YAN H, ZHANG C B, ZHANG J Y, PENG B, ZHAO L M . Effects of density and nitrogen application amount on seed-setting rate and seed production yield of soybean cytoplasmic male sterile line. Journal of Jilin Agricultural University, 2016,38(6):671-674. (in Chinese)
[27]	张伟, 张伟龙, 张春宝, 赵丽梅, 李洪来, 彭宝, 张井勇, 闫昊, 邱强, 赵婧 . 一种提高杂交大豆制种产量的父母本种植方法: 中国, CN104106353A. 2014-10-22.
	ZHANG W, ZHANG W L, ZHANG C B, ZHAO L M, LI H L, PENG B, ZHANG J Y, YAN H, QIU Q, ZHAO J . A parental planting method to increase the seed yield of hybrid soybean: China,CN104106353A. 2014-10-22. (in Chinese)
[28]	ZHANG J Y, SUN H, ZHAO L M, ZHANG C M, YAN H, PENG B, LI W B . Nectar secretion of RN-type cytoplasmic male sterility three lines in soybean [Glycine max(L.) Merr.]. Journal of Integrative Agriculture, 2018,17(5):1085-1092.
[29]	张井勇, 赵丽梅, 彭宝, 张春宝, 张伟龙, 闫昊, 孙寰 . 雌性育性对大豆细胞质雄性不育系异交率的影响//第九届全国大豆学术讨论会, 2013: 5, 49.
	ZHANG J Y, ZHAO L M, PENG B, ZHANG C B, ZHANG W L, YAN H, SUN H . Effect of female fertility on the outcrossing rate of male sterile lines with RN sterile cytoplasm//The Ninth National Soybean Symposium, 2013: 5, 49. (in Chinese)
[30]	BERNARD R L, CREMEENS C R . Inheritance of the Eldorado male-sterile trait.Soybean Genetics. Newsletter, 1975,2:37-39.
[31]	宋启建, 吴天侠, 盖钧镒 . 大豆隐性核不育基因的遗传与应用研究若干问题探讨. 大豆科学, 1995,14(2):137-142.
	SONG Q J, WU T X, GAI J Y . Application of soybean male sterility to population improvement. Soybean Science, 1995,14(2):137-142. (in Chinese)
[32]	赵团结, 盖钧镒 . 大豆不育性自然变异的发现与鉴定. 中国农业科学, 2006,39(9):1756-1764.
	ZHAO T J, GAI J Y . Detection and identification of soybean natural variation of sterility. Scientia Agricultura Sinica, 2006,39(9):1756-1764. (in Chinese)
[33]	赵团结, 杨守萍, 盖钧镒 . 大豆显性核雄性不育突变体N7241S的发现与遗传分析. 中国农业科学, 2005,38(1):22-26.
	ZHAO T J, YANG S P, GAI J Y . Discovery of a dominant nuclear male sterile mutant N7241S in Soybean and analysis of iIts inheritance. Scientia Agricultura Sinica, 2005,38(1):22-26. (in Chinese)
[34]	李曙光, 赵团结, 盖钧镒 . 大豆突变体NJS-1H核雄性不育性的细胞学与遗传学分析. 大豆科学, 2010,29(2):181-185.
	LI S G, ZHAO T J, GAI J Y . Cytological and genetical characterization of a nuclear male 2sterille soybean mutant NJS21H. Soybean Science, 2010,29(2):181-185. (in Chinese)
[35]	ZHAO T J, GAI J Y . Discovery of new male-sterile cytoplasm sources and development of a new cytoplasmic-nuclear male-sterile line NJCMS3A in soybean. Eupytica, 2006,152(3):387-396. doi: 10.1007/s10681-006-9226-0
[36]	IBM Corp. IBM SPSS Statistics for Windows. Version 19.0 . Armonk, NY, USA: IBM Corp, 2010.
[37]	张井勇, 赵丽梅, 孙寰, 彭宝, 李文滨 . 大豆不育系育性稳定性研究概况. 大豆科学, 2015,34(4):712-716.
	ZHANG J Y, ZHAO L M, SUN H, PENG B, LI W B . A review of fertility stability in male sterility for soybean. Soybean Science, 2015,34(4):712-716. (in Chinese)
[38]	张井勇, 孙寰, 赵丽梅, 彭宝, 张伟龙, 李曙光, 赵晓明 . 大豆RN不育胞质不育与恢复类型的研究. 大豆科学, 2010,29(4):559-564.
	ZHANG J Y, SUN H, ZHAO L M, PENG B, ZHANG W L, LI S G, ZHAO X M . The classification of male-sterile lines with RN sterile cytoplasm and their restorers. Soybean Science, 2010,29(4):559-564. (in Chinese)
[39]	白羊年, 陈健, 喻德跃, 盖钧镒 . 大豆雄性不育系和大豆资源有关开花授粉性状的研究. 大豆科学, 2002,21(1):18-24.
	BAI Y N, CHEN J, YU D Y, GAI J Y . A study on pollination- related traits in soybean male- sterile lines and soybean germplasm. Soybean Science, 2002,21(1):18-24. (in Chinese)
[40]	张井勇, 赵丽梅, 孙寰, 彭宝, 张伟, 张春宝, 张伟龙, 闫昊, 李慧, 王鹏年 . 一种高效大规模鉴定大豆种质对传粉昆虫吸引力的方法: 中国, CN106962176A. 2017-07-21.
	ZHANG J Y, ZHAO L M, SUN H, PENG B, ZHANG W, ZHANG C B, YAN H, LI H, WANG P N . A large scale efficient evaluating method of attraction to pollination Insect on soybean: China,CN106962176A. 2017-07-21. (in Chinese)

亲本 Parents	“三系”类别 Type of three lines	高异交率 High outcrossing rate		中异交率 Middle outcrossing rate		低异交率 Low outcrossing rate
母本 Female parent	不育系 Male-Sterile Lines	JLCMS82A	JLCMS101A	JLCMS9A	JLCMS47A	JLCMS89A	JLCMS31A
母本 Female parent	保持系 Maintainers	JLCMS82B	JLCMS101B	JLCMS9B	JLCMS47B	JLCMS89B	JLCMS31B
父本 Male parent	恢复系 Restorers	JLCMSR2

材料名 Material	年份 Year			平均 Average
材料名 Material	2012	2013	2014	平均 Average
JLCMS101A	48.39±3.68	41.67±4.53	58.33±8.66	49.46±8.94a
JLCMS82A	47.65±5.83	37.33±7.26	52.33±5.93	45.44±8.49a
JLCMS9A	37.50±6.96	31.67±5.20	39.83±6.68	36.33±6.58b
JLCMS47A	28.67±5.83	27.84±7.32	34.17±6.5	30.22±6.43c
JLCMS31A	17.33±3.65	15.16±4.65	19.50±4.07	17.33±4.05d
JLCMS89A	13.83±5.70	13.33±3.42	20.67±5.73	15.94±5.64d
平均Average	32.23±14.62b	27.83±11.82c	37.47±15.95a

材料名 Material	年份 Years			平均 Average
材料名 Material	2012	2013	2014	平均 Average
JLCMS101A	37.29±6.28	29.22±4.56	32.73±5.17	33.08±5.83a
JLCMS82A	35.41±4.15	28.40±2.37	30.30±5.66	31.37±4.85ab
JLCMS47A	31.68±3.41	26.47±4.81	24.66±4.70	27.60±4.92bc
JLCMS9A	30.43±3.27	23.77±4.35	24.65±3.62	26.28±4.53c
JLCMS89A	23.59±4.08	16.37±3.28	21.36±6.34	20.44±5.21d
JLCMS31A	18.63±3.29	20.20±3.46	20.84±3.44	19.89±3.10d
平均Average	29.51±7.58a	24.07±5.73b	25.76±6.12b

材料名 Material	工人1 Person 1			工人2 Person 2			工人3 Person 3			平均Average
材料名 Material	授粉花数The flowers pollinated	收获荚数The pods obtained	成活率 Survival rate (%)	授粉花数The flowers pollinated	收获荚数The pods obtained	成活率 Survival rate (%)	授粉花数The flowers pollinated	收获荚数The pods obtained	成活率 Survival rate (%)	授粉花数 The flowers pollinated	收获荚数 The pods obtained	成活率 Survival rate (%)
JLCMS101A	198	104	52.53	208	121	58.17	186	110	59.14	197.33	111.67	56.61a
JLCMS82A	198	114	57.58	208	118	56.73	186	97	52.15	197.33	109.67	55.49a
JLCMS9A	198	107	54.04	208	96	46.15	186	88	47.31	197.33	97.00	49.17b
JLCMS47A	198	91	45.96	208	93	44.71	186	95	51.08	197.33	93.00	47.25ab
JLCMS47B	198	87	43.94	208	100	48.08	186	98	52.69	197.33	95.00	48.23ab
JLCMS89B	198	94	47.47	208	95	45.67	186	89	47.85	197.33	92.67	47.00ab
JLCMS9B	198	95	47.98	208	93	44.71	186	83	44.62	197.33	90.33	45.77ab
JLCMS101B	198	90	45.45	208	95	45.67	186	80	43.01	197.33	88.33	44.71abc
JLCMS31B	198	84	42.42	208	92	44.23	186	86	46.24	197.33	87.33	44.30abc
JLCMS82B	198	90	45.45	208	86	41.35	186	80	43.01	197.33	85.33	43.27bcd
JLCMS89A	198	79	39.90	208	80	38.46	186	80	43.01	197.33	79.67	40.46cd
JLCMS31A	198	72	36.36	208	83	39.90	186	74	39.78	197.33	76.33	38.68d

材料名 Material	工人1 Person1			工人2 Person2			工人3 Person3			平均 Average
材料名 Material	授粉花数The flowers pollinated	异交荚数The pods obtained	成活率 Survival rate (%)	授粉花数The flowers pollinated	异交荚数The pods obtained	成活率 Survival rate (%)	授粉花数The flowers pollinated	异交荚数The pods obtained	成活率 Survival rate (%)	授粉花数 The flowers pollinated	异交荚数The pods obtained	成活率 Survival rate (%)
JLCMS82A	203	125(1)	61.58	177	106(2)	59.89	186	123	66.13	188.67	118.00	62.53a
JLCMS101A	203	131(3)	64.53	177	108(2)	61.02	186	108(1)	58.06	188.67	115.67	61.20a
JLCMS101B	203	119 (13)	58.62	177	97(11)	54.80	186	115(9)	61.83	188.67	110.33	58.42ab
JLCMS47B	203	112(12)	55.17	177	90(9)	50.85	186	121(13)	65.05	188.67	107.67	57.02ab
JLCMS9B	203	99(8)	48.77	177	99(10)	55.93	186	115(11)	61.83	188.67	104.33	55.51ab
JLCMS82B	203	114(11)	56.16	177	93(7)	52.5	186	112(10)	60.22	188.67	106.33	56.31ab
JLCMS31B	203	119(9)	58.62	177	73(10)	41.24	186	121(12)	65.05	188.67	104.33	54.97ab
JLCMS89B	203	106(10)	52.22	177	84(11)	47.46	186	117(13)	62.90	188.67	102.33	54.19ab
JLCMS9A	203	102	50.25	177	81	45.76	186	101	54.30	188.67	94.67	50.10bc
JLCMS47A	203	105	51.72	177	88	49.72	186	88	47.31	188.67	93.67	49.58bc
JLCMS31A	203	87	42.86	177	65	36.72	186	78	41.94	188.67	76.67	40.51cd
JLCMS89A	203	80(1)	39.41	177	72	40.68	186	65	34.95	188.67	81.67	38.35d