,bagging,transcriptome,high-throughput sequencing,functional classification,"/> 遮光性套袋对桃果实转录组的影响

中国农业科学 ›› 2017, Vol. 50 ›› Issue (6): 1088-1097.doi: 10.3864/j.issn.0578-1752.2017.06.010

• 园艺 • 上一篇    下一篇

遮光性套袋对桃果实转录组的影响

何平,李林光,王海波,常源升,李慧峰   

  1. 山东省果树研究所,山东泰安 271000
  • 收稿日期:2016-07-01 出版日期:2017-03-16 发布日期:2017-03-16
  • 通讯作者: 李林光,Tel:0538-8266675;E-mail:llg6536@163.com
  • 作者简介:何平,E-mail:heping024@163.com
  • 基金资助:
    国家现代农业产业技术体系(CARS-31)、山东省农业重大应用技术创新课题(2014-38)、山东省农业科学院农业科技创新工程(CXGC2016B07)

Effects of Shading Fruit with Opaque Paper Bag on Transcriptome in Peach

HE Ping, LI LinGuang, WANG HaiBo, CHANG YuanSheng, LI HuiFeng   

  1. Shandong Institute of Pomology, Tai’an 271000, Shandong
  • Received:2016-07-01 Online:2017-03-16 Published:2017-03-16

摘要: 【目的】探明遮光性套袋在桃果实上的转录组差异,丰富桃转录组数据信息。【方法】选取遮光性套袋与对照的桃果实样品,利用Illumina HiSeqTM 2500进行高通量测序,构建桃果实转录组文库,并用测序评估、基因功能注释等生物信息学方法进行分析。【结果】经过测序获得16.62 Gb clean data测序数据,且碱基百分比(Q30)大于91%,遮光性套袋和对照2个桃果实样品分别获得65 300 730个reads和66 603 686个reads,分别有85.73%和84.60%的reads与桃参考基因组匹配。以无袋处理为参考,对转录组数据进行比较,遮光性套袋处理共获得1 963个差异表达基因,其中,下调基因708个,上调基因1 255个;在Nr数据库中对差异基因进一步进行注释,注释到1 957个基因,其中,下调基因有705个,上调基因有1 252个;COG功能注释分析发现这些差异表达基因共获得853个功能注释,涉及23个功能类别;在GO功能注释分析中,注释到1 609个基因,可以分为53个功能分类,这些分类主要涉及到分子结合、催化活性、细胞过程、生物调节等诸多生理生化过程;KEGG分析发现共有421个基因被注释到94个代谢通路中,其中,光合作用相关通路、类黄酮生物合成、核糖体生物合成等通路显著富集。光合作用通路和类黄酮生物合成通路在果实着色中发挥了重要作用,而核糖体生物合成代谢通路在果实成熟着色中的作用尚不明确。同时也进行了两组样品的果实品质检测,结果表明,遮光性套袋对桃果实的可溶性固形物及可溶性总糖产生显著性影响,可溶性固形物及可溶性总糖显著降低,而对于果实总酸的影响不大,在果实大小上几乎没有影响。【结论】在遮光性套袋处理状态下,获得一定数量的桃果实差异表达基因,光合作用通路和类黄酮生物合成通路基因在果实着色中发挥重要作用,遮光性套袋对桃果实的可溶性固形物及可溶性总糖产生显著性影响。

关键词: 桃, 遮光, 套袋, 转录组, 高通量测序, 功能分类

Abstract: Objective The objective of this study was to find the transcriptome differences between shading fruits with opaque paper bags and CK in peach, and enrich the peach transcriptome data. Method Selecting peach fruits as samples (shading fruits with opaque paper bags and CK, respectively), then the transcriptome libraries of peach fruits were constructed by using the Illumina HiSeqTM 2500 sequencing technique, and were analyzed by using the bioinformatics methods subsequently, such as sequencing assess and gene function annotation. Result The results showed that the 16.62 Gb transcriptome data were obtained from both the shading bagged fruits and CK , and the base ratios with quality values higher than 30 in reads (Q30) were more than 91% from both the shading fruits and CK. There were 65 300 730 reads and 66 603 686 reads transcriptome data were obtained from the peach fruits used bagging or not, and 85.73% and 84.60% reads could be compared to the peach reference genome sequence, respectively. Compared the transcriptome of shading bagged fruits with CK, 1 963 differentially expressed genes were found in the shading bagged fruits, including 1 255 up-regulated genes and 708 down-regulated genes. Annotation analysis indicated that 1 957 genes were annotated in Nr data, including 1 252 up-regulated genes and 705 down-regulated genes. Through COG analysis, there were 853 functional annotations of these differentially expressed genes, involving 23 functional classifications. With GO function annotation classifications, a total of 1 609 genes were divided into 53 function categories, in which many functional categories were mainly involved, such as molecular binding, catalytic activity, cell process, biological regulation. KEGG analysis showed that a total of 421 genes were annotated to 94 metabolic pathways, and photosynthesis signaling pathway, carbon fixation in photosynthetic organisms, photosynthesis-antenna proteins signaling pathway, flavonoid biosynthesis, ribosome biosynthesis (DNA replication) pathway were significantly enriched. The photosynthesis and flavonoid biosynthesis signaling pathway played an important role in fruit color, but the role of ribosome biosynthesis was not clear. The fruit quality also was detected from the shading bagged fruits and CK. The results showed that shading bagged peach fruits had a remarkable impact on soluble solids and total soluble sugar, which were significantly reduced. But the study also found, on average, little or no effect on total acid and per fruit weight from both the shading fruits and CK. Conclusion The number of differentially expressed genes under different physiological status of the fruit tissue in peach were obtained. The study found that the photosynthesis and flavonoid biosynthesis signaling pathway played an important role in fruit color and shading bagged peach fruits had a remarkable impact on soluble solids and total soluble sugar.

Key words: peach, ')">shading, bagging')">bagging, transcriptome, high-throughput sequencing, functional classification