利用CDDP标记的菏泽牡丹品种资源的遗传多样性

doi:10.3864/j.issn.0578-1752.2013.13.012

摘要/Abstract

摘要： 【目的】在品种群水平和花色群体水平上分析菏泽牡丹资源的遗传多样性和遗传分化，为该资源的保护和利用提供理论依据与技术支持。【方法】利用CDDP分子标记技术，对白、粉、黑、红、黄、蓝、绿、紫红、紫和复色系等10个花色群体构成的299份菏泽牡丹品种资源的基因组DNA进行扩增分析。【结果】用18条CDDP引物对10个花色群体共299份样品进行扩增，共检测到385条扩增带，其中多态性条带368条，特异条带23条，分别占总条带的95.58%和5.97%。在品种群水平上，Nei’s基因多样性指数、有效等位基因数和Shannon信息指数分别为0.1648、1.2569和0.2695；在花色群体水平上，上述指标依次为0.1451、1.2313和0.2306。综合分析各项指标得出，红色系和紫色系具有较高的遗传多样性，复色系和绿色系牡丹的遗传多样性较低。花色群体间的遗传距离较近，平均为0.0271；遗传一致度较高，平均为0.9735；遗传分化系数为0.1252，表明只有12. 52%的遗传分化存在于群体间；群体间还具有较大基因流值（3.4939）。遗传多样性分析和UPGMA聚类结果在分子水平上验证了牡丹品种资源的花色演化趋势：以粉色系和红色系为中心，渐渐演化出紫红、紫、蓝和白色系，再进化为黄色系和黑色系，绿色系和复色系属于退化的色系。【结论】CDDP技术可有效揭示牡丹种质资源的遗传多样性。在品种群水平上，菏泽牡丹品种资源遗传多样性高于花色群体水平。花色群体间存在一定程度的遗传分化。聚类结果揭示了牡丹花色的演化趋势。

关键词: 牡丹 , 花色 , CDDP , 遗传多样性 , 遗传分化

Abstract: 【Objective】The objective of this study was to evaluate the genetic diversity and differentiation of Heze tree peony resources at cultivar group level and flower-color population level, respectively.【Method】DNA samples of 299 tree peony cultivars composed of 10 different flower-color populations were amplified by CDDP primers. The 10 populations respectively consisted of white, pink, black, red, yellow, blue, green, fuchsia, purple and complex color cultivars.【Result】With 18 informative and reliable primers, 368 polymorphic loci were detected out of 385 bands in total. At cultivar group level, the Nei’s gene diversity (H), Shannon’s information index (I) and effective number of alleles were 0.1648, 1.2569 and 0.2695, respectively. At population level, the above index was 0.1451, 1.2313 and 0.2306 in turn. Comprehensive analysis indicated that the red population or purple population had higher genetic diversity, while the genetic diversity of the complex-color population or green population was lower. The genetic distance among 10 populations was closer, with an average of 0.0271. The genetic identity was higher, with an average of 0.9735. The coefficient of gene differentiation (Gst) among them was 0.1252, showing that 12.52% genetic variation existed among populations. Gene flow from Gst was 3.4939. Results obtained from genetic diversity analysis and UPGMA dendrogram based Nei’s genetic identity proved the evolutionary trend of tree peony’s flower color at molecular level: pink and red systems might be at the center of Heze tree peony resources, then gradually evolved into fuchsia, purple, blue, white, yellow or black cultivars, while green or complex color cultivars belonged to the degraded color system.【Conclusion】CDDP marker technique can effectively reveal the genetic diversity of tree peony germplasm resources. At cultivar group level, the diversity of Heze tree peony resources was higher than that at flower-color population level. There existed a certain degree of genetic differentiation among the 10 populations. And the clustering results revealed the evolutionary trend of tree peony color.

Key words: tree peony , flower color , CDDP , genetic diversity , genetic differentiation

李莹莹, 郑成淑. 利用CDDP标记的菏泽牡丹品种资源的遗传多样性[J]. 中国农业科学, 2013, 46(13): 2739-2750.

LI Ying-Ying, ZHENG Cheng-Shu. Genetic Diversity of Tree Peony Cultivar Resources in Heze Revealed by CDDP Marker[J]. Scientia Agricultura Sinica, 2013, 46(13): 2739-2750.

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