体外法优化肉鸡日粮非淀粉多糖酶

doi:10.3864/j.issn.0578-1752.2012.21.014

摘要/Abstract

摘要： 【目的】探讨非淀粉多糖酶对肉鸡玉米日粮和小麦日粮离体消化能的调控。【方法】首先采用单因素随机试验设计，测定非淀粉多糖酶与日粮离体消化能的剂量关系。5种非淀粉多糖（non-starch polysaccharides，NSP）酶分别为：木聚糖酶、β-葡聚糖酶、纤维素酶、果胶酶和β-甘露聚糖酶，在肉鸡玉米-豆粕和小麦-豆粕日粮中分别添加0、100、200、300、400、500、600、700、800和900 µg•g-1，分析NSP酶改善日粮离体消化能的作用效果。然后采用二次回归旋转正交组合试验设计，筛选两种日粮中5种NSP酶的最佳酶谱。离体消化能的测定采用胃蛋白酶-胰液素两步酶水解法。【结果】5种非淀粉多糖酶对离体消化能的调控效应显示规律性的“S”型曲线，木聚糖酶对玉米-豆粕、小麦-豆粕日粮的离体消化能提升程度较高，分别提高0.24和0.40 MJ•kg-1。在本试验条件下，玉米-豆粕日粮的最佳酶谱为：木聚糖酶34 836.4 U•kg-1、β-葡聚糖酶6 762.0 U•kg-1、纤维素酶1 159.2 U•kg-1、果胶酶872.6 U•kg-1和β-甘露聚糖酶24 535.9 U•kg-1；小麦-豆粕日粮的最佳酶谱为：木聚糖酶65 405.5 U•kg-1、β-葡聚糖酶10 131.5 U•kg-1、纤维素酶980.0 U•kg-1、果胶酶501.6 U•kg-1和β-甘露聚糖酶5 141.4 U•kg-1。【结论】肉鸡玉米-豆粕日粮、小麦-豆粕日粮中5种NSP酶联合使用能够改善日粮的离体消化能。

关键词: 非淀粉多糖酶, 离体消化能, 酶谱, 肉仔鸡, 体外法

Abstract: 【Objective】The study was conducted to discuss the effects of non-starch polysaccharides (NSP) enzymes on the in vitro digestive energy (IVDE) of broiler diets of different contents of NSP.【Method】The first step was to investigate the dose-response of the NSP enzymes on the IVDE in diets using one-way randomized experimental design. Five NSP enzymes including xylanase, β-glucanase, cellulose, pectinase, and β-mannanase were added into corn-soybean diet and wheat-soybean diet, from 0 to 900 µg•g-1. The second step was to screen the optimum enzyme combinations using quadratic regress-orthogonal rotary design. The method of testing IVDE was a two-stage enzymic hydrolysis with pepsin-pancreatin in vitro.【Result】The IVDE of broiler diet regulating by NSP enzyme appeared S state diagram. The xylanase had more promotion effects than other NSP enzymes. It increased IVDE by 0.24 MJ•kg-1 and 0.40 MJ•kg-1 in corn-soybean diet and wheat-soybean diet, respectively. In corn-soybean diet, the optimum enzyme combinations were xylanase 34 836.4 U•kg-1, β-glucanase 6 762.0 U•kg-1, cellulose 1 159.2 U•kg-1, pectase 872.6 U•kg-1, and β-mannanase 24 535.9 U•kg-1. In wheat-soybean diet, the optimum enzyme combinations were xylanase 65 405.5 U•kg-1, β-glucanase 10 131.5 U•kg-1, cellulose 980.0 U•kg-1, pectase 501.6 U•kg-1, and β-mannanase 5 141.4 U•kg-1. 【Conclusion】The findings showed that using five kinds of NSP enzymes in corn-soybean and wheat-soybean diets of broiler could improve the in vitro digestive energy.

Key words: NSP enzyme, in vitro digestive energy, enzyme combination, broiler, in vitro

何科林, 萨仁娜, 高杰, 李东卫, 庄晓峰, 张宏福. 体外法优化肉鸡日粮非淀粉多糖酶[J]. 中国农业科学, 2012, 45(21): 4457-4464.

HE Ke-Lin, SA Ren-Na, GAO Jie, LI Dong-Wei, ZHUANG Xiao-Feng, ZHANG Hong-Fu. Study on Optimization of Non-Starch Polysaccharide Enzymes of Broiler Diets in Vitro[J]. Scientia Agricultura Sinica, 2012, 45(21): 4457-4464.

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