中国农业科学 ›› 2008, Vol. 41 ›› Issue (3): 834-840 .doi: 10.3864/j.issn.0578-1752.2008.03.028

• 畜牧·资源昆虫 • 上一篇    下一篇

静脉注射不同形态锰源对肉仔鸡生物学活性的影响

李素芬,罗绪刚,吕 林,刘 彬,卜友泉,余顺祥   

  1. 中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室
  • 收稿日期:2006-11-08 修回日期:2007-02-07 出版日期:2008-03-10 发布日期:2008-03-10
  • 通讯作者: 罗绪刚

Bioavailability of intravenously injected manganese sources for broilers

  

  1. 中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室
  • Received:2006-11-08 Revised:2007-02-07 Online:2008-03-10 Published:2008-03-10

摘要: 【目的】研究经静脉注射途径进入体内的不同形态锰在肉仔鸡体内代谢利用上的差异。【方法】将180只22日龄肉公鸡按体重随机分为5个处理组,分别翅静脉注射生理盐水,或在生理盐水中溶解硫酸锰及弱、中等和强络合强度有机锰源的注射液20 d。【结果】静脉注射后第10和20天,各注射锰源组鸡的跖骨灰锰含量、心肌锰含量、心肌细胞线粒体中MnSOD活性和MnSOD mRNA均显著(P<0.03)高于注射生理盐水的对照组,但前3项指标未能恒定一致地反映出不同形态锰对肉仔鸡的生物学活性的差异。注射中等络合强度锰源组心肌细胞线粒体中MnSOD mRNA水平显著高于注射硫酸锰组(P<0.01)和强络合强度锰源组(P<0.09),且注射弱络合强度锰源组心肌细胞线粒体中MnSOD mRNA水平显著高于注射硫酸锰组(P<0.06)。【结论】注射锰在转录水平上显著影响心肌细胞线粒体中MnSOD的基因表达, 这一功能性基因表达指标比其酶活性等其它指标更敏感、更恒定地监测出肉仔鸡对不同锰间生物学利用性的差异。经静脉注射途径进入体内的中等和弱络合强度有机锰源对肉仔鸡的生物学活性显著高于无机锰,而强络合强度有机锰源不利于肉仔鸡体组织细胞对其中锰的利用。

关键词: 静脉注射, MnSOD mRNA水平, 锰源, 肉仔鸡

Abstract: [Objective] Differences in Mn metabolic utilization between organic and inorganic Mn sources or among organic Mn sources for broilers were detect. [Method] Broilers on 22 day-old were randomly allotted to one of five treatments and injected with 0.9% NaCl solution (Control), or the 0.9% NaCl solution dissolved with Mn sulfate, or organic Mn source with weak, moderate, or strong chelation stength for 20 days. [Result] On d 10 and 20 after injection, chicks injected with Mn containing solution had a significantly higher metatarsal bone ash Mn content, heart Mn content, heart MnSOD activity, and MnSOD mRNA level than those in control group (P < 0.03), but the indices except MnSOD mRNA level could not consistently detect the differences among Mn sources. Chicks injected with solution containing organic Mn sources with moderate chelation strength had higher heart MnSOD mRNA level than those injected with Mn sulfate (P < 0.01) and organic Mn sources with strong chelation strength (P<0.09), and chicks injected with solution containing organic Mn sources with weak chelation strength had higher heart MnSOD mRNA level than those injected with Mn sulfate (P < 0.06). [Conclusion] The results indicated that intravenously injected Mn could significantly affect heart MnSOD gene expression trancriptionally, and this functional index could more rapidly, sensitively and consistently detect the difference among Mn sources than other indices. Intravenously injected Mn from the organic Mn source with moderate and weak chelation strength was utilized and functioned in the sensitive target tissue of broilers more effectively than Mn from Mn sulfate, and the organic Mn source with the strong complex strength would be not good for tissue utilization.