黑龙江省大豆灰斑病菌生理小种及遗传关系分析

doi:10.3864/j.issn.0578-1752.2012.21.006

中国农业科学 ›› 2012, Vol. 45 ›› Issue (21): 4377-4387.doi: 10.3864/j.issn.0578-1752.2012.21.006

黑龙江省大豆灰斑病菌生理小种及遗传关系分析

丁俊杰, 顾鑫, 杨晓贺, 赵海红, 申宏波, 姜翠兰, 仕相林, 刘春燕, 胡国华, 陈庆山

1.黑龙江省农业科学院佳木斯分院/农业部佳木斯作物有害生物科学观测实验站，黑龙江佳木斯 154007
2.黑龙江省农垦科学院作物所，黑龙江佳木斯 154007
3. 黑龙江农垦科研育种中心，哈尔滨 150030
4. 东北农业大学农学院，哈尔滨 150030

收稿日期:2012-03-16 出版日期:2012-11-01 发布日期:2012-05-29
通讯作者: 通信作者胡国华，Tel：13304608757；E-mail：hugh757@vip.163.com；通信作者陈庆山，Tel：0451-55191945；E-mail：qshchen@sohu.com
作者简介:丁俊杰，Tel：0454-8351067；E-mail：me999@126.com
基金资助:
黑龙江省自然科学基金项目（C200936）、国家公益性行业（农业）科研专项（201103016-03A2)

Analysis of Race and Genetic Relationship of Cercospora sojina in Heilongjiang Province

DING Jun-Jie, GU Xin, YANG Xiao-He, ZHAO Hai-Hong, SHEN Hong-Bo, JIANG Cui-Lan, SHI Xiang-Lin, LIU Chun-Yan, HU Guo-Hua, CHEN Qing-Shan

1.黑龙江省农业科学院佳木斯分院/农业部佳木斯作物有害生物科学观测实验站，黑龙江佳木斯 154007
2.黑龙江省农垦科学院作物所，黑龙江佳木斯 154007
3. 黑龙江农垦科研育种中心，哈尔滨 150030
4. 东北农业大学农学院，哈尔滨 150030

Received:2012-03-16 Online:2012-11-01 Published:2012-05-29

摘要/Abstract

摘要： 【目的】调查黑龙江省大豆灰斑病菌（Cercospora sojina）的生理小种类型，分析黑龙江省大豆灰斑病菌各生理小种间的亲缘关系。【方法】2006—2011年，对黑龙江省进行大豆灰斑病样本的采集和病菌的分离。利用11对SSR引物对黑龙江省的24个灰斑病菌菌株进行EST-SSR基因型分析。【结果】共分离获得大豆灰斑病菌2 674株，鉴定出1—15号共15个生理小种。SSR结果共检测出等位变异46个，平均每个位点为4.2个，相似系数范围为0.091—0.956，平均相似性系数达到0.589。聚类分析结果与大豆灰斑病菌小种类型呈现出较高的相关性。【结论】黑龙江省大豆灰斑病菌间具有丰富的遗传多样性。大豆灰斑病菌的亲缘关系与生理小种类型密切相关，与采集年份、采集地点相关性不大。

关键词: 大豆灰斑病菌, 生理小种, 基因型

Abstract: 【Objective】The objective of this study is to survey race types of Cercospora sojina and to analyze the genetic relationship of races of C. sojina in Heilongjiang Province. 【Method】Samples of C. sojina were collected and pathogens were isolated in Heilongjiang Province from 2006 to 2011. Twenty-four strains of C. sojina were analyzed by EST-SSR with 11 pairs SSR primers. 【Result】Fifteen races were identified from 2 674 strains of C. sojina obtained in this study. The results of SSR showed that the amount of the allelic variation in all strains was 46, and the average was 4.2. Similarity index between strains ranged from 0.091 to 0.956, and the average was 0.589. The cluster results had the high relativity to race of C. sojina strains.【Conclusion】There was a rich genetic diversity among C. sojina in Heilongjiang Province. The genetic relationship of C. sojina is closely related to race type, and is irrelevant to collective time and place.

Key words: Cercospora sojina, race, genotype

丁俊杰, 顾鑫, 杨晓贺, 赵海红, 申宏波, 姜翠兰, 仕相林, 刘春燕, 胡国华, 陈庆山. 黑龙江省大豆灰斑病菌生理小种及遗传关系分析[J]. 中国农业科学, 2012, 45(21): 4377-4387.

DING Jun-Jie, GU Xin, YANG Xiao-He, ZHAO Hai-Hong, SHEN Hong-Bo, JIANG Cui-Lan, SHI Xiang-Lin, LIU Chun-Yan, HU Guo-Hua, CHEN Qing-Shan. Analysis of Race and Genetic Relationship of Cercospora sojina in Heilongjiang Province[J]. Scientia Agricultura Sinica, 2012, 45(21): 4377-4387.

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黑龙江省大豆灰斑病菌生理小种及遗传关系分析

Analysis of Race and Genetic Relationship of Cercospora sojina in Heilongjiang Province

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