两个中国小麦品种中抗叶锈基因的遗传分析和基因定位

doi:10.3864/j.issn.0578-1752.2012.16.006

摘要/Abstract

摘要： 【目的】确定来自四川的两个小麦品种绵阳351-15和SW8588所携带的抗叶锈基因，为选育持久抗锈品种提供理论依据。【方法】在苗期用15个叶锈菌生理小种接种小麦品种绵阳351-15、SW8588和30个含有已知抗叶锈基因的近等基因系，推导2个材料中所含有的抗叶锈病基因，同时以小麦抗叶锈品种绵阳351-15和SW8588分别同感病品种郑州5389杂交获得F1和F2代群体，用叶锈菌小种FHTT接种各亲本及其杂交后代，进行抗叶锈遗传分析，并利用SSR和STS标记进行抗叶锈病基因的分子定位。【结果】经苗期基因推导发现SW8588中含有未知基因不同于已知抗叶锈病基因Lr1，绵阳351-15中可能含有已知抗叶锈病基因Lr1。用叶锈菌小种FHTT接种各F1和F2代群体，2个F2代群体抗感单株分离比例均符合3﹕1的理论分离比例，表明2个亲本对小种FHTT的抗病性均由1个显性基因控制。经过分子标记分析，在小麦材料绵阳351-15中发现该抗叶锈基因与位于5DL的SSR标记barc144和wmc765连锁，其遗传距离分别为8.9和20.8 cM，并同Lr1的STS标记WR003共分离，确定在小麦材料绵阳351-15中对小种FHTT的抗病性由抗叶锈基因Lr1提供；经分子标记检测SW8588中含有1对显性的抗叶锈病基因，暂命名为LrSW85，该基因位于5DL染色体上与Lr1的STS标记WR003共分离，该抗叶锈基因可能是Lr1的等位基因或紧密连锁基因。【结论】通过基因推导、遗传分析和分子标记等手段，确定小麦材料绵阳351-15中含有抗叶锈基因Lr1；小麦材料SW8588中含有抗叶锈基因LrSW85，该基因可能为Lr1的等位基因或紧密连锁基因。

关键词: 小麦抗叶锈基因, 基因定位, SSR标记, STS标记

Abstract: 【Objective】 The objective of this study is to identify the leaf rust resistance genes of two Chinese wheat (Triticum aestivum L.) lines SW8588 and Mianyang 351-15 from Sichuan province, and to provide a basis for breeding wheat cultivars with durable resistance. 【Method】 Mianyang 351-15, SW8588 and 30 differential lines with known leaf rust resistance genes were inoculated with 15 pathotypes of Puccinia triticina for postulating leaf rust resistance genes in the two lines. The two F1 and F2 populations from crosses of Mianyang 351-15/Zhengzhou 5389 and SW8588/Zhengzhou 5389, were inoculated with pathotype FHTT in the greenhouse. The infection types were scored 15 days after inoculation and molecular markers were also used for mapping the resistance genes.【Result】SW8588 did not contain the known leaf rust resistance gene Lr1 and Mianyang 351-15 maybe contain Lr1 and other unknown genes. When inoculating with pathotype FHTT, only one resistance gene was detected in each of the two resistance lines. Using SSR makers, the resistance gene in Mianyang 351-15 was mapped on 5DL and linked to SSR markers barc144 and wmc765 with genetic distances of 8.9 and 20.8 cM, respectively. The STS marker WR003 for Lr1 was co-segregated with this resistance gene in Mianyang 351-15. Based on these results, it was concluded that Mianyang 351-15 contained Lr1 conferring the resistance to FHTT. Using genetic analysis and molecular markers it could be found that SW8588 carried a single dominant resistance gene, temporarily designated LrSW85, co-segregating with STS marker WR003, which may be an allele of Lr1 or was closely linked to Lr1.【Conclusion】Based on gene postulation, genetic analysis and molecular mapping it can be concluded that Mianyang 351-15 contained the leaf rust resistance gene Lr1 and SW8588 contained the leaf rust resistance gene LrSW85 which may be an allele of Lr1 or closely linked to Lr1.

Key words: wheat leaf rust resistance gene, gene mapping, SSR marker, STS marker

周悦, 吴娱, 李星, 李在峰, 刘大群. 两个中国小麦品种中抗叶锈基因的遗传分析和基因定位[J]. 中国农业科学, 2012, 45(16): 3273-2380.

ZHOU Yue, WU Yu, LI Xing, LI Zai-Feng, LIU Da-Qun. Genetic Analysis and Molecular Mapping of Leaf Rust Resistance Genes in Two Chinese Wheat Lines[J]. Scientia Agricultura Sinica, 2012, 45(16): 3273-2380.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2012.16.006

https://www.chinaagrisci.com/CN/Y2012/V45/I16/3273

参考文献

[1] Denissen C J M. Influence of race and post infection temperature on two components of partial resistance to wheat leaf in seedlings of wheat. Euphytica, 1991, 58(1): 13-20.

[2] 董金皋. 农业植物病理学. 北京: 中国农业出版社, 2001: 44-45.

Dong J G. Plant Pathology in Agriculture. Beijing: China Agriculture Press, 2001: 44-45. (in Chinese)

[3] Kolmer J A. Genetics of resistance to wheat leaf rust. Annual Review of Phytopathology, 1996, 34: 435-455.

[4] McIntosh R A, Dubcovsky J, Rogers W J, Morris C F, Appels R, Xia X C. Catalogue of gene symbols for wheat: 2010 supplement. Annual Wheat Newsletter, 2010, 56: 273-283.

[5] Kilpatrick R A. New wheat cultivars and longevity of rust resistance, 1971-1975. Beltsville, MD: US Department of Agriculture, Agricultural Research Service, 1975: 20.

[6] Watson I A, Singh D. The future for rust resistant wheat in Australia. Journal of the Australian Institute of Agricultural Science, 1952, 18: 190-197.

[7] Eriksen L, Afshari F, Christiansen M J, Mclntosh R A, Jahoor A, Wellings C R. Yr32 for resistance to stripe (yellow) rust present in the wheat cultivar Carstens V. Theoretical and Applied Genetics, 2004, 108(3): 567-575.

[8] McIntosh R A, Lagudah E S. Cytogenetical studies in wheat. XVIII. Gene Yr24 for resistance to stripe rust. Plant Breeding, 2000, 119(1): 81-93.

[9] Wan A M, Zhao Z H, Chen X M, He Z G, Jin S H, Jia Q Z, Yao G, Yang J X, Wang B T, Li G B, Bi Y Q, Yuan Z Y. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Disease, 2004, 88: 896-904.

[10] Li Z F, Xia X C, He Z H, Li X, Zhang L J, Wang H Y, Meng Q F, Yang W X, Li G Q, Liu D Q. Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars. Plant Disease, 2010, 94: 45-53.

[11] Zhao X L, Zheng T C, Xia X C, He Z H, Liu D Q, Yang W X, Yin G H, Li Z F. Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theoretical and Applied Genetics, 2008, 117(7): 1069-1075.

[12] Zhang H, Xia X C, He Z H, Li X, Li Z F, Liu D Q. Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16. Molecular Breeding, 2011, 28(4): 527-534.

[13] 陈现朝, 李星, 李在峰, 陈欢, 高敏, 刘大群. 中国小麦贵州98-18中抗叶锈基因的分子定位. 植物病理学报, 2010, 40(5): 489-494.

Chen X C, Li X, Li Z F, Chen H, Gao M, Liu D Q. Molecular mapping of leaf rust resistance gene LrG98 in Chinese wheat line Guizhou98-18. Acta Phytopathologica Sinica, 2010, 40(5): 489-494. (in Chinese)

[14] 李星, 李在峰, 李亚宁, 赵志泉, 刘大群, 王翠芬, 高丽娇, 孙道杰. 小麦品系西农1163-4 抗叶锈病基因的遗传分析和分子作图. 中国农业科学, 2010, 43(12): 2397-2402.

Li X, Li Z F, Li Y N, Zhao Z Q, Liu D Q, Wang C F, Gao L J, Sun D J. Genetic analysis and molecular mapping of leaf rust resistance gene in wheat line Xinong 1163-4. Scientia Agricultura Sinica, 2010, 43(12): 2397-2402. (in Chinese)

[15] Sacco F, Suarez E Y, Naranjo T. Mapping of the leaf rust resistance gene Lr3 on chromosome 6B of Sinvalocho MA wheat. Genome, 1998, 41(5): 686-690.

[16] 杨文香, 刘大群. 小麦抗叶锈基因的定位及分子标记研究进展. 中国农业科学, 2004, 37(1): 65-71.

Yang W X, Liu D Q. Advances in localization and molecular markers of wheat leaf rust resistance genes. Scientia Agricultura Sinica, 2004, 37(1): 65-71. (in Chinese)

[17] Khan R R, Bariana H S, Dholakia B B, Naik S V, Lagu M D, Rathjen A J, Bhavani S, Gupta V S. Molecular mapping of stem and leaf rust resistance in wheat. Theoretical and Applied Genetics, 2005, 111(5): 846-850.

[18] Helguera M, Vanzetti L, Soria M, Khan I A, Kolmer J, Dubcovsky J. PCR markers for Triticum speltoides leaf rust resistance gene Lr51 and their use to develop isogenic hard red spring wheat lines. Crop Science, 2005, 45(2): 728-734.

[19] Hiebert C, Thomas J, McCallum B. Locating the broad-spectrum wheat leaf rust resistance gene Lr52 (LrW) to chromosome 5B by a new cytogenetic method. Theoretical and Applied Genetics, 2005, 110(8): 1453-1457.

[20] McIntosh R A, Devos K M, Dubcovsky J, Rogers W J, Morris C F, Appels R, Somers D J, Anderson O A. Catalogue of gene symbols for wheat: 2008 Supplement. Annual Wheat Newsletter, 2008, 54: 209-225.

[21] McIntosh R A, Dubcovsky J, Rogers W J, Morris C F, Appels R, Xia X C. Catalogue of gene symbols for wheat: 2009 Supplement. Annual Wheat Newsletter, 2009, 55: 256-278.

[22] Li Z F, Xia X C, Zhou X C, Niu Y C, He Z H, Zhang Y, Li G Q, Wan A M, Wang D S, Chen X M, Lu Q L, Singh R P. Seedling and slow rusting resistance to stripe rust in chinese common wheats. Plant Disease, 2006, 90: 1302-1312.

[23] Rajaram S, Hettel G P. Wheat Breeding at CIMMYT: Commemorating 50 Years of Research in Mexico for Global Wheat Improvement. Mexico: International Maize and Wheat Improvement Center, 1994: 52-53.

[24] Long D L, Kolmer J A. A north American system of nomenclature for Puccinia recondita f. sp. tritici. Phytopathology, 1989, 79: 525-529.

[25] Dubin H J, Johnson R, Stubbs R W. Postulated genes for resistance to strip rust in selected CIMMYT and related wheats. Plant Disease, 1989, 73(6): 472-475.

[26] Sharp P J, Kreis M, Shewry P R, Gale M D. Location of β-amylase sequence in wheat and its relatives. Theoretical and Applied Genetics, 1988, 75: 286-290.

[27] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the United States of America, 1991, 88(21): 9828-9832.

[28] Qiu J W, Schürch A C, Yahiaoui N, Dong L L, Fan H J, Zhang Z J, Keller B, Ling H Q. Physical mapping and identification of a candidate for the leaf rust resistance gene Lr1 of wheat. Theoretical and Applied Genetics, 2007, 115(2): 159-168.

[29] Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M H, Leroy P, Ganal M W. A microsatellite map of wheat. Genetics, 1998, 149(4): 2007-2023.

[30] 齐爱勇, 李星, 赵振杰. 中国小麦LB0288中抗叶锈病基因的鉴定. 中国农学通报, 2011, 27(12): 52-55.

Qi A Y, Li X, Zhao Z J. Identification of leaf rust resistance genes in wheat line LB0288. Chinese Agricultural Science Bulletin, 2011, 27(12): 52-55. (in Chinese)

[31] 袁军海, 刘太国, 陈万全. 中国47个小麦新品种(系)苗期抗叶锈基因推导. 中国农业科学, 2007, 40(9): 1925-1935.

Yuan J H, Liu T G, Chen W Q. Postulation of leaf rust resistance genes in 47 new wheat cultivars (lines) at seedling stage. Scientia Agricultura Sinica, 2007, 40(9): 1925-1935. (in Chinese)

[32] 周悦, 李在峰, 李星, 王龙, 张晔, 刘大群. 小麦品系天95HF2 抗叶锈基因定位. 作物学报, 2010, 36(8): 1265-1269.

Zhou Y, Li Z F, Li X, Wang L, Zhang Y, Liu D Q. Molecular mapping of leaf rust resistance genes in wheat line Tian 95HF2. Acta Agronmica Sinica, 2010, 36(8): 1265-1269. (in Chinese)