中国农业科学 ›› 2012, Vol. 45 ›› Issue (9): 1826-1832.doi: 10.3864/j.issn.0578-1752.2012.09.018

• 畜牧·资源昆虫 • 上一篇    下一篇

鸡miR-181a组织的表达谱及其转录调控区域

 孙桂荣, 李明, 康相涛, 李国喜, 田亚东, 韩瑞丽, 白义春   

  1. 1.河南农业大学牧医工程学院/河南省家禽种质资源创新工程研究中心,郑州 450002
  • 收稿日期:2011-09-07 出版日期:2012-05-01 发布日期:2011-12-01
  • 通讯作者: 通信作者康相涛,E-mail:xtkang2001@263.net
  • 作者简介:孙桂荣,E-mail:grsun2000@126.com
  • 基金资助:

    国家自然科学基金项目(31072023)、农业部现代农业产业技术体系建设专项资金(CARS-41-k04)

The Difference Expression Profiles of miR-181a and Transcription Regulation Region Analysis in Chicken

 SUN  Gui-Rong, LI  Ming, KANG  Xiang-Tao, LI  Guo-Xi, TIAN  Ya-Dong, HAN  Rui-Li, BAI  Yi-Chun   

  1. 1.河南农业大学牧医工程学院/河南省家禽种质资源创新工程研究中心,郑州 450002
  • Received:2011-09-07 Online:2012-05-01 Published:2011-12-01

摘要: 【目的】分析鸡miR-181a的转录起始位点,并分析其在1日龄和成年鸡不同组织中的表达谱,以期为研究miR-181a的功能奠定基础。【方法】采用qPCR技术构建miR-181a 在1日龄和成年鸡肝脏、脾脏、肺、小肠、大脑、小脑、下丘脑、脑干、胸腺、心脏和肾脏组织中的表达谱。用version(1.83)分析miR-181a的保守性,并采用ENCODE数据库进行miR-181a转录起始位点和启动子区域的分析。【结果】①采用qPCR检测miR-181a在鸡下丘脑中的表达量和高通量测序的结果一致,1日龄下丘脑中的表达量显著低于成年鸡的表达量(P<0.05);②miR-181a 在11个组织中均有表达,且在1日龄和成年鸡脾脏、肺、小肠、大脑、小脑、下丘脑、脑干、胸腺、心脏和肾脏组织的表达量差异显著(P<0.05),而在肝脏中表达量的差异不显著(P>0.05);③miR-181a的前体miR-181a-1上游区域有一个启动子区和一个潜在的转录起始位点;④miR-181a-1的转录调控区域内存在的多个转录因子,转录因子主要有NFKB,c-Fos等。【结论】鸡miR-181a的表达有组织和时序差异,其成熟序列在脊椎动物中十分保守,上游有一个转录起始位点及启动子区。

关键词: 鸡, miR-181a, 转录起始位点

Abstract: 【Objective】 In order to provide theoretical data support for the study of the function of miR-181a, the tissue and developmental expression profiles, as well as transcriptional regulation region of chicken miR-181a were analyzed. 【Method】 The expression patterns of chicken miR-181a at two stages of chicken cerebrum lung, liver, spleen, kindey, crura, muscle, hupothalamus, cerebellum, heart, pectoralmuscle and thymus gland tissues were performed with qPCR technology, and the miR-181a transcriptional regulation site and the promoter region were analyzed by ENCODE. 【Result】 The results indicated that the expression of miR-181a in hypothalamus from the solexa sequencing was similar to those from qPCR. The results of qPCR showed that: the expression of miR-181a was tissue-specific in the same developmental period. The expression level of miR-181a was significantly different in different tissues. The expression in the same tissue between one day and adult chicken showed phase specific, the expression of miR-181a in other ten tissues was significantly different (P<0.05), except liver (P>0.05). ENCODE showed that in the upstream region of miR-181a-1 there existed continuous transcriptional regulation regions, ChIP-seq showed that the transcriptional regulatory region of miR-181a-1 may exist a transcriptional factor. The main transcription factors are NFKB, c-Fos and so on. 【Conclusion】The result indicated that the expression of miR-181a was tissue- and phase-specific. The sequence of miR-181a is highly conservative in vertebrates. The upstream of miR-181a has a transcriptional regulation region and a promoter region.

Key words: chicken, miR-181a, transcription regulation region