青山羊表皮生长因子基因表达与毛囊发育特性的研究

doi:10.3864/j.issn.0578-1752.2012.06.016

摘要/Abstract

摘要： 【目的】比较研究表皮生长因子（epidermal growth factor，EGF）基因的表达与青山羊皮肤毛囊发育之间的关系。【方法】用组织学技术和显微观测的方法，研究济宁青山羊1日龄、1—5月龄，共6个年龄段的皮肤毛囊的组织学特性，结合荧光定量PCR技术，研究其与EGF基因表达之间的关系，并用SAS9.0软件进行相关性分析。【结果】初级毛囊直径在出生后随日龄稍有增大，但差异不显著（P＞0.05），而次级毛囊直径在2月龄之前极显著增大（P＜0.01），达到发育高峰，之后保持相对稳定。EGF基因在出生后表达量极显著升高（P＜0.01），在2月龄达到最高，之后表达量极显著减少（P＜0.01）。【结论】初级毛囊在出生前就已全部发育形成，而次级毛囊在出生后可继续发育至2月龄。自出生至2月龄，EGF表达量与次级毛囊直径呈极显著正相关（P＜0.01）。

关键词: 济宁青山羊, 毛囊发育, 表皮生长因子, 定量PCR

Abstract: 【Objective】 The relationship between the expression of epidermal growth factor and the development of hair follicle was studied.【Method】 The histology method, micro-observation approach and QRT-PCR were used to detect the relationship between the expression of EGF and the development of hair follicle of 1-day-old, 1-month-old, 2-month-old, 3-month-old, 4-month-old and 5-month-old Jining Gray goat. SAS9.0 was used to analyze the relationship between them.【Result】 The resuts indicated that the diameter of primary follicle increased, but not significantly (P＞0.05). The diameter of secondary follicle increased very significantly (P＜0.01). The diameter of secondary follicle reached peak of growth at 2-month-old. The expression of EGF increased after birth and achieved the highest at 2-month-old (P＜0.01). It decreased extremely significant after 2-month-old (P＜0.01).【Conclusion】 The primary follicle was completely developed before birth and the secondary follicle was developed after birth. The expression of EGF and the diameter of secondary follicle had a significant positive correlation before 2-month-old (P＜0.01). The results indicated that EGF is significantly positive to the development of secondary follicle.

Key words: Jining Gray goat, hair follicle development, epidermal growth factor, quantitative PCR

王睿智, 尹逊河, 王慧, 成子强. 青山羊表皮生长因子基因表达与毛囊发育特性的研究[J]. 中国农业科学, 2012, 45(6): 1176-1182.

WANG Rui-Zhi, YIN Xun-He, WANG Hui, CHENG Zi-Qiang. Studies on Expression Characteristics of EGF and Development Characteristics of Hair Follicle of Gray Goat[J]. Scientia Agricultura Sinica, 2012, 45(6): 1176-1182.

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