中国农业科学 ›› 2012, Vol. 45 ›› Issue (1): 16-25.doi: 10.3864/j.issn.0578-1752.2012.01.003

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

SRAP结合ISSR方法分析黄麻属的起源与演化

 陶爱芬, 祁建民, 李木兰, 方平平, 林荔辉, 徐建堂   

  1. 1.福建农林大学作物遗传育种与综合利用教育部重点实验室/福建农林大学生命科学学院,福州 350002
  • 收稿日期:2011-03-31 出版日期:2012-01-01 发布日期:2011-11-11
  • 通讯作者: 通信作者祁建民,Tel:0591-87644898;E-mail:qijm863@163.com
  • 作者简介:陶爱芬,E-mail:281770126@qq.com
  • 基金资助:

    国家自然科学基金项目(31000734)、国家麻类产业技术体系项目(CARS-19-E06)、南方麻类种质资源平台建设项目(2010N2002)

Origin and Evolution of Jute Analysed by SRAP and ISSR Methods

 TAO  Ai-Fen, QI  Jian-Min, LI  Mu-Lan, FANG  Ping-Ping, LIN  Li-Hui, XU  Jian-Tang   

  1. 1.福建农林大学作物遗传育种与综合利用教育部重点实验室/福建农林大学生命科学学院,福州 350002
  • Received:2011-03-31 Online:2012-01-01 Published:2011-11-11

摘要: 【目的】以SRAP结合ISSR分子标记方法从分子生物学角度分析黄麻属的起源与演化。【方法】以来自13个国家的黄麻属6个种共96份种质资源为供试材料,采用SRAP结合ISSR标记的方法,用MEGA软件结合DPS软件绘制黄麻属的进化树,并计算黄麻属各类型的进化时间。【结果】在供试黄麻种质资源中,近缘野生种黄麻处于进化树的最基础位置,且平均进化时间最长,是黄麻2个栽培种最原始的祖先类型。非洲为黄麻属野生种的起源与演化中心,中国是世界黄麻属的次生中心。非洲的野生长果种黄麻与栽培长果种黄麻起源均最早,为世界长果种黄麻的初生中心;印度-缅甸-中国毗邻地区为世界长果种黄麻的次生中心。中国南部以及与之相邻的南亚、东南亚地区为世界野生圆果种黄麻的起源中心,中国南部地区同时为世界栽培圆果种黄麻的起源中心。栽培圆果种黄麻的平均进化时间较短,说明圆果栽培种黄麻在进化上较长果种黄麻稍迟。【结论】非洲在黄麻属起源与演化上有重要地位,是世界黄麻属野生种和野生长果种黄麻及栽培长果种黄麻的起源中心;中国南部地区是世界栽培圆果种黄麻的起源中心。用SRAP与ISSR分子标记结合的方法,可较全面地计算黄麻属各类型的进化时间和绘制进化树,从而得到关于黄麻属起源与演化的较科学的结论。

关键词: 黄麻, 起源, 演化, SRAP, ISSR

Abstract: 【Objective】The objective of this study is to make clear the origin and evolution of Corchorus with two molecular methods (SRAP and ISSR). 【Method】Ninety-six jute germplasms were analysed with sequence related amplified polymorphism (SRAP) combining with inter-simple sequence related (ISSR) method. The phylogenetic trees of Corchorus were constructed by MEGA and DPS software, and the divergence time of jute germplasm was calculated. 【Result】 The relative wild species located at the basic position of the dendrogram, and the divergence time of which was the longest, which indicated the relative wild species originated earliest and was the ancestors of cultivated jute. Africa was the center of origin of Corchorus, while China was the second original center of Corchorus. Among all the olitorius species, the divergence time of wild and cultivated species from Africa was the longest, so Africa was the center of origin for wild and cultivated olitorius species, while the areas which border on India, Burma and China was the second center of origin for cultivated olitorius species. South China, countries of South Asia and Southeast Asia which border on China were the origin center of wild capsularis species, and South region of China was the origin center of cultivated capsularis species. Taken together, the divergence time of the cultivated capsularis species was shorter than olitorius species, indicating this biotype originated later than olitorius species. 【Conclusion】 Africa has an important position in origin of Corchorus, which was the primary origin center of wild species, wild and cultivated olitorius species. South region of China was the origin center of cultivated capsularis species. The divergence time and phylogenetic trees of Corchorus were calculated more comprehensively by SRAP markers combining with ISSR molecular markers, and the scientific conclusion on origin and evolution of Corchorus was got with above methods.

Key words: Corchorus, origin, evolution, SRAP, ISSR