中国农业科学 ›› 2019, Vol. 52 ›› Issue (5): 822-836.doi: 10.3864/j.issn.0578-1752.2019.05.005
收稿日期:
2018-10-27
接受日期:
2018-12-23
出版日期:
2019-03-01
发布日期:
2019-03-12
通讯作者:
石洁
作者简介:
常佳迎,E-mail: 基金资助:
CHANG JiaYing,LIU ShuSen,MA HongXia,SHI Jie(),GUO Ning,ZHANG HaiJian
Received:
2018-10-27
Accepted:
2018-12-23
Online:
2019-03-01
Published:
2019-03-12
Contact:
Jie SHI
摘要: 【目的】 针对黄淮海地区发生的玉米弯孢叶斑病,通过分子生物学技术,明确其致病菌新月弯孢(Curvularia lunata )在不同地区和年份间的遗传差异及亲缘关系,为研究该病害的发生和流行提供数据资料。【方法】 对2013、2016和2017年采集自黄淮海地区5省(河南、河北、山东、安徽、江苏)的病样进行分离,并采用形态学和分子生物学(ITS和EF-1α 序列分析)对分离到的菌株进行鉴定,共获得175个新月弯孢菌株。从哥伦比亚大学开发的通用引物中筛选出13条多态性高、重复性好的引物,利用筛选出的引物对175个新月弯孢菌株进行ISSR-PCR扩增,利用Popgen32软件计算多态性比率、Shannon’s信息指数、群体间的遗传距离和遗传相似性,使用NTsys2.10e软件进行UPGMA聚类分析和基于遗传相似系数的主坐标分析,构建聚类分析图和散点图。【结果】 利用筛选出的引物对175个菌株进行PCR扩增,共获得105条多态性条带,多态性比率为100%。在群体平均水平上,基因多样性水平(H)为0.3867,Shannon’s的信息指数(I)为0.5682,表明玉米弯孢叶斑病菌具有丰富的遗传多样性;不同地理种群间的遗传多样性存在一定差异,河南和安徽种群遗传多样性最高,江苏种群较低;年度间相同地理来源的菌群亲缘关系较远,相同年份不同地理来源菌群亲缘关系较近。聚类分析显示所有菌株相似系数为0.51—0.93,在相似系数为0.59水平上,175个菌株被划分为2群5个亚群,亚群间表现出年度间的差异,不同地理种群病菌间存在基因交流,遗传相关性较高;主坐标分析结果与聚类分析结果一致,同一年份的菌株明显聚集在一起。【结论】 引起黄淮海地区玉米弯孢叶斑病的病原菌群体存在较高的遗传变异,地域相邻的病菌遗传关系较近;同一地区的菌株在年度间表现出一定遗传距离,而同一年份不同地理来源的菌株遗传距离较近。引起该地区玉米弯孢叶斑病的新月弯孢菌株不是以本地菌源为主,其主要菌源可能来自南方水稻和草坪草或东南亚玉米生产区,但也存在少量存活于地表病残体上的菌株可作翌年的初侵染源。
常佳迎,刘树森,马红霞,石洁,郭宁,张海剑. 黄淮海地区夏玉米弯孢叶斑病菌遗传多样性分析[J]. 中国农业科学, 2019, 52(5): 822-836.
CHANG JiaYing,LIU ShuSen,MA HongXia,SHI Jie,GUO Ning,ZHANG HaiJian. Genetic Diversity Analysis of Curvularia lunata in Summer Maize in Huang-Huai-Hai Region[J]. Scientia Agricultura Sinica, 2019, 52(5): 822-836.
表1
供试菌株"
采集年份 Year | 采集地点 Collection site | 菌株编号 Strain number | |||
---|---|---|---|---|---|
2013 | 安徽 Anhui | 宿州Suzhou | 萧县马井镇Majing, Xiaoxian | AH1301-1, AH1301-2 | |
灵璧县灵城镇Lingcheng, Lingbi | AH1301-6—AH1301-9 | ||||
宿县黄口镇Huangkou, Suxian | AH1301-10 | ||||
淮北Huaibei | 濉溪县孙疃镇Suntuan, Suixi | AH1301-3—AH1301-5 | |||
亳州Bozhou | 蒙城县许疃镇Xutuan, Mengcheng | AH1302-1—AH1302-3 | |||
蒙城县东土镇Dongtu, Mengcheng | AH1302-4 | ||||
蒙城县板桥集镇Banqiaoji, Mengcheng | AH1302-5, AH1302-6 | ||||
蒙城县王集乡Wangji, Mengcheng | AH1302-7—AH1302-9 | ||||
利辛县巩店镇Gongdian, Lixin | AH1302-10, AH1302-11 | ||||
利辛县汝集镇Ruji, Lixin | AH1302-12—AH1302-15 | ||||
河南 He’nan | 驻马店Zhumadian | 确山县朱古洞乡Zhugudong, Queshan | HN1301-1—HN1301-4 | ||
确山县三里河乡Sanlihe, Queshan | HN1301-5 | ||||
泌阳县盘古乡Pangu, Miyang | HN1301-6—HN1301-9 | ||||
泌阳县高店乡Gaodian, Miyang | HN1301-10 | ||||
遂平县车站镇Chezhan, Suiping | HN1301-11—HN1301-16 | ||||
遂平县和兴乡Hexing, Suiping | HN1301-17—HN1301-19 | ||||
周口Zhoukou | 商水县姚集乡Yaoji, Shangshui | HN1302-1 | |||
商水县城关镇Chengguan, Shangshui | HN1302-2 | ||||
沈丘县白集镇Baiji, Shenqiu | HN1302-3—HN1302-5 | ||||
沈丘县北校乡Beixiao, Shenqiu | HN1302-6—HN1302-8 | ||||
平顶山Pingdingshan | 叶县龚店乡Gongdian, Yexian | HN1303-1, HN1303-2 | |||
叶县叶邑镇Yeyi, Yexian | HN1303-3, HN1303-4 | ||||
叶县田庄乡Tianzhuang, Yexian | HN1303-5, HN1303-6 | ||||
许昌Xuchang | 许昌县尚积镇Shangji, Xuchang | HN1304-1, HN1304-2 | |||
许昌县蒋李集镇Jiangliji, Xuchang | HN1304-3, HN1304-4 | ||||
开封Kaifeng | 兰考县城关镇Chengguan, Lankao | HN1305-1, HN1305-2 | |||
商丘Shangqiu | 拓城县胡囊镇Hu’nang, Tuocheng | HN1306-1, HN1306-2 | |||
2016 | 安徽Anhui | 宿州Suzhou | 埇桥区Yongqiao | AH1601-1 | |
河南 He’nan | 商丘Shangqiu | 夏邑县曹集乡Caoji, Xiayi | HN1606-1 | ||
周口Zhoukou | 太康县张集镇Zhangji, Taikang | HN1602-1, H1N602-2 | |||
漯河Luohe | 舞阳县孟寨镇Mengzhai, Wuyang | HN1607-1, HN1607-2 | |||
开封Kaifeng | 杞县李庄村Lizhuang, Qixian | HN1605-1, HN1605-2 | |||
兰考县城关乡Chengguan, Lankao | HN1605-3 | ||||
郑州Zhengzhou | 荥阳县高村乡Gaocun, Xingyang | HN1608-1, HN1608-2 | |||
巩义市河洛镇Heluo, Gongyi | HN1608-3, HN1608-4 | ||||
焦作Jiaozuo | 沁阳市崇义镇Chongyi, Qinyang | HN1609-1 | |||
博爱县清化镇Qinghua, Boai | HN1609-2, HN1609-3 | ||||
修武县郇封镇千仓村Qiancang, Xunfeng, Xiuwu | HN1609-4, HN1609-5 | ||||
武陟县宁郭镇张庄村Zhangzhuang, Ningguo, Wuzhi | HN1609-6 | ||||
采集年份 Year | 采集地点 Collection site | 菌株编号 Strain number | |||
2017 | 安徽 Anhui | 亳州Bozhou | 利辛县垄店镇Gongdian, Lixin | AH1702-1 | |
蒙城县王集乡Wangji, Mengcheng | AH1702-2 | ||||
蒙城县板桥集镇Banqiaoji, Mengcheng | AH1702-3 | ||||
宿州Suzhou | 灵璧县灵城镇Lingcheng, Lingbi | AH1701-1, AH1701-2 | |||
埇桥区大店镇Dadian, Yongqiao | AH1701-3 | ||||
埇桥区Yongqiao | AH1701-4 | ||||
淮北Huaibei | 濉溪县刘桥镇Liuqiao, Suixi | AH1703-2 | |||
相山区Xiangshan | AH1703-3 | ||||
濉溪县南平镇Nanping, Suixi | AH1703-1 | ||||
河北 Hebei | 石家庄Shijiazhuang | 新乐市马头铺镇Matoupu, Xinle | HB1701-1 | ||
赵县赵州镇Zhaozhou, Zhaoxian | HB1701-2 | ||||
藁城区Gaocheng | HB1701-3, HB1701-5, HB1701-6 | ||||
灵寿县北洼乡Beiwa, Lingshou | HB1701-4 | ||||
保定Baoding | 望都韩庄镇Hanzhuang,Wangdu | HB1702-1 | |||
蠡县南庄镇Nanzhuang, Lixian | HB1702-2 | ||||
雄县双堂乡Shuangtang, Xiongxian | HB1702-3 | ||||
蠡县蠡吴镇Liwu, Lixian | HB1702-4 | ||||
高阳县高阳镇Gaoyang, Gaoyang | HB1702-5 | ||||
满城大册营镇Daceying, Mancheng | HB1702-6 | ||||
易县高村镇Gaocun, Yixian | HB1702-7 | ||||
涞源王安镇Wang’an, Laiyuan | HB1702-8 | ||||
易县尉都乡Weidu, Yixian | HB1702-9 | ||||
涞源县胡家庄乡Hujiazhuang, Laiyuan | HB1702-10 | ||||
涿州市松林店镇Songlindian, Zhuozhou | HB1702-11 | ||||
定兴县定兴镇Dingxing, Dingxing | HB1702-12 | ||||
徐水区Xushui | HB1702-13 | ||||
邯郸Handan | 邯郸县丛台区Congtai, Handan | HB1703-1 | |||
峰峰县新坡镇Xinpo, Fengfeng | HB1703-2 | ||||
大城县大尚屯镇Dashangtun, Dacheng | HB1706-3 | ||||
大城县广安镇Guang’an, Dacheng | HB1706-4 | ||||
衡水Hengshui | 武邑县肖桥头镇Xiaoqiaotou, Wuyi | HB1704-3, HB1704-4 | |||
故城县故城镇Gucheng, Gucheng | HB1704-1 | ||||
深州市Shenzhou | HB1704-2 | ||||
沧州Cangzhou | 东光县找王镇Zhaowang, Dongguang | HB1705-1 | |||
青县新兴镇Xinxing, Qingxian | HB1705-2 | ||||
沧县崔尔庄镇Cuierzhuang, Cangxian | HB1705-3 | ||||
河间市时村乡Shicun, Hejian | HB1705-4 | ||||
任丘市麻家坞镇Majiawu, Renqiu | HB1705-5 | ||||
青县曹寺镇Caosi, Qingxian | HB1705-6 | ||||
献县淮镇Huaizhen, Xianxian | HB1705-7 | ||||
廊坊Langfang | 固安县马庄镇Mazhuang, Gu’an | HB1706-1 | |||
固安县Gu’an | HB1706-2 | ||||
固安县牛驼镇Niutuo, Gu’an | HB1706-5 | ||||
采集年份 Year | 采集地点 Collection site | 菌株编号 Strain number | |||
唐山Tangshan | 玉田县杨家套乡Yangjiatao, Yutian | HB1707-1 | |||
昌黎县葛条港乡Getiaogang, Changli | HB1707-2 | ||||
玉田县石臼窝镇Shijiuwo, Yutian | HB1707-3 | ||||
滦州市滦州镇Luanzhou, Luanzhou | HB1707-6 | ||||
滦州市Luanzhou | HB1707-7 | ||||
遵化市团瓢庄乡Tuanpiaozhuang, Zunhua | HB1707-8 | ||||
迁安市Qian’an | 五重安乡Wuchongan | HB1707-4 | |||
赵新庄Zhaoxinzhuang | HB1707-5 | ||||
承德Chengde | 承德县下板城镇Xiabancheng, Chengde | HB1709-1 | |||
秦皇岛Qinhuangdao | 卢龙县Lulong | HB1708-1 | |||
抚宁区Funing | HB1708-2 | ||||
河南 He’nan | 安阳Anyang | 安阳县崔家桥镇Cuijiaqiao, Anyang | HN1710-1 | ||
鹤壁Hebi | 淇县西岗镇Xigang, Qixian | HN1711-1 | |||
新乡Xinxiang | 辉县胡桥乡Huqiao, Huixian | HN1712-1 | |||
新乡县Xinxiang | HN1712-2 | ||||
漯河Luohe | 源汇区Yuanhui | HN1707-1 | |||
驻马店Zhumadian | 西平县二郎镇Erlang, Xiping | HN1701-1 | |||
周口Zhoukou | 西华县田口乡Tiankou, Xihua | HN1702-1 | |||
平顶山Pingdingshan | 宝丰县杨庄镇Yangzhuang, Baofeng | HN1703-1 | |||
商丘Shangqiu | 柘城县Zhecheng | HN1706-1 | |||
江苏 Jiangsu | 徐州Xuzhou | 铜山区大彭镇Dapeng, Tongshan | JS1701-1, JS1701-2 | ||
沛县栖山镇Qishan, Peixian | JS1701-3 | ||||
沛县鹿楼镇Lulou, Peixian | JS1701-4 | ||||
沛县朱寨镇Zhuzhai, Peixian | JS1701-5 | ||||
山东 Shandong | 滨州Binzhou | 阳信县劳店镇Laodian, Yangxin | SD1701-1 | ||
德州Dezhou | 乐陵县Leling | SD1702-1 | |||
平原县Pingyuan | SD1702-2 | ||||
德城区Decheng | SD1702-3 | ||||
济宁Jining | 微山县Weishan | SD1704-1 | |||
曲阜市石门山镇Shimenshan, Qufu | SD1704-2 | ||||
汶上县苑庄镇Yuanzhuang, Wenshang | SD1704-3 | ||||
嘉祥县瞳里镇Tongli, Jiaxiang | SD1704-4 | ||||
任城区Rencheng | SD1704-5 | ||||
枣庄Zaozhuang | 滕州市西岗镇Xigang,Tengzhou | SD1703-1 | |||
峄城区峨山镇Eshan, Yicheng | SD1703-2 | ||||
临沂Linyi | 兰陵县金陵镇Jinling, Lanling | SD1705-1 | |||
兰山区汪沟镇Wanggou, Lanshan | SD1705-2 | ||||
莱芜Laiwu | 莱芜区杨庄镇Yangzhuang, Laiwu | SD1706-1 | |||
泰安Taian | 东平县彭集镇Pengji, Dongping | SD1707-1 | |||
菏泽Heze | 鄄城县红船镇Hongchuan, Juancheng | SD1708-1 | |||
聊城Liaocheng | 聊城市东昌府区Dongchangfu, Liaocheng | SD1709-1 | |||
潍坊Weifang | 临朐县Linqu | SD1710-1 |
表2
ISSR引物扩增"
引物 Primer | 序列 Sequence (5′-3′) | 退火温度 Tm (℃) | 扩增条带数 Number of amplified bands | 多态性条带 Number of polymorphic bands | 多态性比率 Polymorphism ratio (%) |
---|---|---|---|---|---|
808 | (AG)8C | 49 | 10 | 10 | 100 |
810 | (GA)8T | 45 | 7 | 7 | 100 |
823 | (TC)8C | 50 | 6 | 6 | 100 |
827 | (AC)8G | 52 | 7 | 7 | 100 |
835 | (AG)8YC | 53 | 10 | 10 | 100 |
841 | (GA)8YC | 50 | 11 | 11 | 100 |
842 | (GA)8YG | 50 | 10 | 10 | 100 |
847 | (CA)8RC | 54 | 6 | 6 | 100 |
855 | (AC)8YT | 46 | 7 | 7 | 100 |
873 | (GACA)4 | 49 | 7 | 7 | 100 |
880 | (GGAGA)3 | 46 | 7 | 7 | 100 |
888 | BDB(CA)7 | 48 | 9 | 9 | 100 |
891 | HVH(TG)7 | 47 | 8 | 8 | 100 |
总计Total | 105 | 105 | 100 |
表3
新月弯孢不同地理种群的遗传多样性水平"
多态性位点 Number of polymorphic loci | 多态性比率 Polymorphism ratio (%) | 观察等位基因数 Observed number of alleles (NA) | 有效等位基因 Effective number of alleles (NE) | 基因多样性指数Nei’s gene diversity (H) | Shannon’s信息指数 Shannon’s information index (I) | |
---|---|---|---|---|---|---|
安徽AH | 103 | 98.10 | 1.9810 | 1.6344 | 0.3665 | 0.5428 |
河南HN | 105 | 100.00 | 2.0000 | 1.6309 | 0.3662 | 0.5438 |
河北HB | 96 | 91.43 | 1.9143 | 1.5855 | 0.3415 | 0.5072 |
江苏JS | 58 | 55.24 | 1.5524 | 1.3904 | 0.2170 | 0.3171 |
山东SD | 90 | 85.71 | 1.8571 | 1.5355 | 0.3109 | 0.4623 |
群体水平 Population level | 105 | 100.00 | 2.0000 | 1.6761 | 0.3867 | 0.5682 |
表4
新月弯孢不同地理种群间的遗传相似性和遗传距离"
种群Group | 安徽AH13 | 河南HN13 | 安徽AH16 | 河南HN16 | 安徽AH17 | 河北HB17 | 河南HN17 | 江苏JS17 | 山东SD17 |
---|---|---|---|---|---|---|---|---|---|
安徽AH13 | 0.9869 | 0.6550 | 0.8571 | 0.7644 | 0.8767 | 0.8621 | 0.7855 | 0.8652 | |
河南HN13 | 0.0132 | 0.6467 | 0.8559 | 0.7682 | 0.8829 | 0.8623 | 0.7854 | 0.8669 | |
安徽AH16 | 0.4232 | 0.4358 | 0.7510 | 0.6771 | 0.6893 | 0.6751 | 0.6316 | 0.6953 | |
河南HN16 | 0.1542 | 0.1557 | 0.2863 | 0.7542 | 0.8292 | 0.7847 | 0.7424 | 0.8173 | |
安徽AH17 | 0.2687 | 0.2637 | 0.3899 | 0.2821 | 0.8698 | 0.9014 | 0.9435 | 0.8947 | |
河北HB17 | 0.1316 | 0.1246 | 0.3720 | 0.1873 | 0.1395 | 0.8924 | 0.8809 | 0.9491 | |
河南HN17 | 0.1483 | 0.1481 | 0.3929 | 0.2424 | 0.1038 | 0.1139 | 0.8897 | 0.9221 | |
江苏JS17 | 0.2415 | 0.2415 | 0.4594 | 0.2978 | 0.0582 | 0.1269 | 0.1168 | 0.9014 | |
山东SD17 | 0.1448 | 0.1429 | 0.3635 | 0.2018 | 0.1112 | 0.0522 | 0.0811 | 0.1038 |
[1] |
戴法超, 高卫东, 吴仁杰, 金晓华 .一种值得注意的玉米病害——弯孢菌叶斑病. 植物病理学报, 1995,25:330.
doi: 10.1007/BF02007173 |
DAI F C, GAO W D, WU R J, JIN X H . A noticeable corn disease: Curvularia leaf spot. Acta Phytopathologica Sinica , 1995,25:330. (in Chinese)
doi: 10.1007/BF02007173 |
|
[2] | 戴法超, 高卫东, 王晓鸣, 吴仁杰, 金晓华 .玉米弯孢菌叶斑病的初步研究简报. 植物保护, 1996,22(4):36-37. |
DAI F C, GAO W D, WANG X M, WU R J, JIN X H . A preliminary report on Curvularia leaf spot of maize. Plant Protection , 1996,22(4):36-37. (in Chinese) | |
[3] |
王晓鸣, 戴法超, 朱振东 .玉米弯孢菌叶斑病的发生与防治. 植保技术与推广, 2003,23(4):37-39.
doi: 10.3969/j.issn.1672-6820.2003.04.023 |
WANG X M, DAI F C, ZHU Z D . Occurrence and control of Curvularia leaf spot in maize. Plant Protection Technology and Extension , 2003,23(4):37-39. (in Chinese)
doi: 10.3969/j.issn.1672-6820.2003.04.023 |
|
[4] |
李富华, 叶华智, 王玉涛, 熊绵平 .玉米弯孢叶斑病的研究进展. 玉米科学, 2004,12(2):97-101, 107.
doi: 10.3969/j.issn.1005-0906.2004.02.030 |
LI F H, YE H Z, WANG Y T, XIONG M P . The research progress of maize Curvularia leaf spot disease. Journal of Maize Sciences , 2004,12(2):97-101, 107. (in Chinese)
doi: 10.3969/j.issn.1005-0906.2004.02.030 |
|
[5] |
GAUTAM MURTY S, PATEL F, PUNWAR B S, PATEL M, SINGH A S, FOUGAT R S .Comparison of RAPD, ISSR, and DAMD markers for genetic diversity assessment between accessions ofJatropha curcas L. and its related species. Journal of Agricultural Sciences Technology , 2013,15:1007-1022.
doi: 10.1684/agr.2013.0625 |
[6] |
文静 .ISSR分子标记技术在植物病原菌研究中的应用. 安徽农业科学, 2010,38(36):20658-20660.
doi: 10.3969/j.issn.0517-6611.2010.36.060 |
WEN J . Application of ISSR molecular marker technique in plant pathogens research. Journal of Anhui Agricultural Sciences , 2010,38(36):20658-20660. (in Chinese)
doi: 10.3969/j.issn.0517-6611.2010.36.060 |
|
[7] |
HARITHA G, SUDHAKAR T, CHANDRA D, RAM T, DIVYA B, SARLA N .Informative ISSR markers help identify genetically distinct accessions ofOryza rufipogon in yield improvement. Rice Science , 2016,23(5):225-241.
doi: 10.1016/j.rsci.2016.08.001 |
[8] |
李海生 .ISSR分子标记技术及其在植物遗传多样性分析中的应用. 生物学通报, 2004,39(2):19-21.
doi: 10.3969/j.issn.0006-3193.2004.02.008 |
LI H S . ISSR molecular marker technology and its application in plant genetic diversity analysis. Bulletin of Biology , 2004,39(2):19-21. (in Chinese)
doi: 10.3969/j.issn.0006-3193.2004.02.008 |
|
[9] | 李六英, 窦彦霞, 马冠华, 陈娅, 林凡力, 王秋月 .我国烟草赤星病菌遗传多样性的ISSR分析. 植物保护学报, 2018,45(4):846-855. |
LI L Y, DOU Y X, MA G H, CHEN Y, LIN F L, WANG Q Y . Genetic diversity analysis of tobacco brown spot pathogens in China by ISSR. Journal of Plant Protection , 2018,45(4):846-855. (in Chinese) | |
[10] |
张小飞, 李晓, 崔丽娜, 邹成佳, 李菁, 龙永昌 .我国玉米灰斑病菌遗传多样性的ISSR分析. 植物保护学报, 2015,42(6):908-913.
doi: 10.13802/j.cnki.zwbhxb.2015.06.007 |
ZHANG X F, LI X, CUI L N, ZOU C J, LI J, LONG Y C . Genetic diversity analysis of Cercospora spp. by ISSR in China.Journal of Plant Protection , 2015,42(6):908-913. (in Chinese)
doi: 10.13802/j.cnki.zwbhxb.2015.06.007 |
|
[11] |
SANKAR A A, MOORE G A .Evaluation of inter-simple sequence repeat analysis for mapping inCitrus and extension of the genetic linkage map. Theoretical and Applied Genetics , 2001,102:206-214.
doi: 10.1007/s001220051637 |
[12] |
BADR A ,EL-SHAZLY H H,SAYED AHMED H I,HAMOUDA M,EL-KHATEEB E,SAKR M. Genetic diversity of Achillea fragrantissima in Egypt inferred from phenotypic variations and ISSR markers associated with traits of plant size and seed yield.Plant Genetic Resources, 2017,15(3):239-247.
doi: 10.1017/S1479262115000568 |
[13] |
范永山, 石文伟, 刘永杰, 张建成, 董金皋 .玉米弯孢霉叶斑病菌的寄生适合度与 RAPD分析. 河北农业大学学报, 2008,31(1):37-41.
doi: 10.3969/j.issn.1000-1573.2008.01.009 |
FAN Y S, SHI W W, LIU Y J, ZHANG J C, DONG J G . Parasitic fitness and RAPD analysis of Curvularia species on corn.Journal of Agricultural University of Hebei , 2008,31(1):37-41. (in Chinese)
doi: 10.3969/j.issn.1000-1573.2008.01.009 |
|
[14] | 张欣芳, 苏前富, 宋淑云, 杜俊岐, 刘影, 张伟, 李红, 晋齐鸣 .东北地区玉米弯孢菌的致病性分化与RAPD分析. 玉米科学, 2010,18(6):122-126. |
ZHANG X F, SU Q F, SONG S Y, DU J Q, LIU Y, ZHANG W, LI H, JIN Q M . Pathogenicity differentiation and RAPD analysis of Curvularia lunata in northeast China. Journal of Maize Sciences , 2010,18(6):122-126. (in Chinese) | |
[15] | 龚国淑, 叶华智, 张敏, 刘萍, 陈希芹, 邵宝林, 余霞, 杨丹玲 .玉米新月弯孢菌(Curvularia lunata )的RAPD分析. 植物病理学报, 2005,35(6):22-27. |
GONG G S, YE H Z, ZHANG M, LIU P, CHEN X Q, SHAO B L, YU X, YANG D L . RAPD analysis among isolates of Curvularia lunata collected from corn in China.Acta Phytopathologica Sinica , 2005,35(6):22-27. (in Chinese) | |
[16] |
王晓东, 高增贵, 姚远, 刘限, 苏家, 李婉莹 .利用UP-PCR、ISSR标记分析玉米弯孢叶斑病菌遗传多样性. 华北农学报, 2014,29(3):227-233.
doi: 10.7668/hbnxb.2014.03.041 |
WANG X D, GAO Z G, YAO Y, LIU X, SU J, LI W Y . Genetic diversity of Curvularia lunata by UP-PCR and ISSR analysis.Acta Agriculturae Boreali-Sinica , 2014,29(3):227-233. (in Chinese)
doi: 10.7668/hbnxb.2014.03.041 |
|
[17] | 张艳菊, 赵珊, 杨森, 周莹莹, 赵伟 .黑龙江省玉米弯孢霉叶斑病病原鉴定. 东北农业大学学报, 2017,48(6):17-23. |
ZHANG Y J, ZHAO S, YANG S, ZHOU Y Y, ZHAO W . Pathogen identification of Curvularia leaf spot of maize in Heilongjiang Province. Journal of Northeast Agricultural University , 2017,48(6):17-23. (in Chinese) | |
[18] |
LIU T, ZHAO F Z, WANG Y Y, HOU J M, LIU L Z, SHEN Y Q, LIU Z, ZHANG H T, ZUO Y H .Comparative analysis of phylogenetic relationships, morphologies, and pathogenicities amongCurvularia lunata isolates from maize in China. Genetics and Molecular Research , 2015,14(4):12537-12546.
doi: 10.4238/2015.October.16.21 pmid: 26505404 |
[19] |
NEI M .Genetic distance between populations. The American Naturalist, 1972,106(949):283-292.
doi: 10.1086/282771 |
[20] |
NEI M .Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America, 1973,70(12):3321-3323.
doi: 10.1073/pnas.70.12.3321 |
[21] | WACHIRA F N, WAUGH R, HACKETT C A, POWELL W .Detection of genetic diversity in tea ( Camellia sinensis ) using RAPD markers. Genome , 1995,38:201-210. |
[22] |
马红霞, 孙华, 郭宁, 张海剑, 石洁, 常佳迎 .禾谷镰孢复合种毒素化学型及遗传多样性分析. 中国农业科学, 2018,51(1):82-95.
doi: 10.3864/j.issn.0578-1752.2018.01.008 |
MA H X, SUN H, GUO N, ZHANG H J, SHI J, CHANG J Y . Analysis of toxigenic chemotype and genetic diversity of the Fusarium graminearum species complex.Scientia Agricultura Sinica , 2018,51(1):82-95. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2018.01.008 |
|
[23] |
郭云燕, 陈茂功, 孙素丽, 武小菲, 江凯, 朱振东, 李洪杰, 何月秋, 王晓鸣 .中国玉米南方锈病病原菌遗传多样性. 中国农业科学, 2013,46(21):4523-4533.
doi: 10.3864/j.issn.0578-1752.2013.21.015 |
GUO Y Y, CHEN M G, SUN S L, WU X F, JIANG K, ZHU Z D, LI H J, HE Y Q, WANG X M .Genetic diversity of Puccinia polysora Underw. in China. Scientia Agricultura Sinic a, 2013,46(21):4523-4533. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2013.21.015 |
|
[24] | 鄢洪海, 王琰, 张茹琴, 夏淑春, 王志奎, 宋希云 .基于ISSR-PCR对山东玉米多堆柄锈菌遗传多样性的研究及其初侵染菌源的推测. 菌物学报, 2018,37(2):157-165. |
YAN H H, WANG Y, ZHANG R Q, XIA S C, WANG Z K, SONG X Y . Genetic diversity and deduction of primary infection source of Puccinia polysora in Shandong Province based on ISSR-PCR.Mycosystema , 2018,37(2):157-165. (in Chinese) | |
[25] | 张定法 .玉米弯孢霉叶斑病初侵染菌源的研究. 河南农业科学,1998(8):24-25. |
ZHANG D F .Research the initial infection of Curvularia leaf spot in maize. Journal of Henan Agricultural Sciences ,1998(8):24-25. (in Chinese) | |
[26] |
暴增海, 马桂珍, 杨文兰, 吕国忠 .玉米弯孢霉叶斑病的初侵染来源及几种杀菌剂的室内毒力测定. 吉林农业大学学报, 2002,24(4):53-57.
doi: 10.3969/j.issn.1000-5684.2002.04.013 |
BAO Z H, MA G Z, YANG W L, LÜ G Z . Primary source of infection of Curvlaria lunata and toxicity of some fungicide to the fungi.Journal of Jilin Agricultural University , 2002,24(4):53-57. (in Chinese)
doi: 10.3969/j.issn.1000-5684.2002.04.013 |
|
[27] | 戴法超, 王晓鸣, 朱振东, 高卫东, 霍纳新, 金晓华 .玉米弯孢菌叶斑病研究. 植物病理学报, 1998,28(2):123-129. |
DAI F C, WANG X M, ZHU Z D, GAO W D, HUO N J, JIN X H . Curvularia leaf spot of maize: Pathogens and varietal resistance. Acta Phytopathologica Sinica , 1998,28(2):123-129. (in Chinese) | |
[28] | 徐辉 .水稻弯孢叶斑病病原鉴定及流行学研究[D].合肥: 安徽农业大学, 2015. |
XU H .Identification of the pathogen and study of epidemiology on rice Curvularia leaf spot[D].Hefei: Anhui Agricultural University, 2015. ( in Chinese) | |
[29] | 金敏忠, 柴荣耀, 张庆生, 林文彩 .水稻黑粒米症状与病原研究初报. 植物保护,1994(2):7-8. |
JIN M Z, CHAI R Y, ZHANG Q S, LIN W C .Preliminary report on symptoms and pathogens of black grain rice.Plant Protection ,1994(2):7-8. (in Chinese) | |
[30] |
李路, 刘连盟, 王国荣, 汪爱娟, 王玲, 孙磊, 黎起秦, 黄世文 .水稻穗腐病和穗枯病的研究进展. 中国水稻科学, 2015,29(2):215-222.
doi: 10.3969/j.issn.10017216.2015.02.014 |
LI L, LIU L M, WANG G R, WANG A J, WANG L, SUN L, LI Q Q, HUANG S W . Research progress of spikelet rot disease and bacterial panicle blight of rice. Chinese Journal of Rice Science , 2015,29(2):215-222. (in Chinese)
doi: 10.3969/j.issn.10017216.2015.02.014 |
|
[31] | 黄微, 夏花, 高必达 .水稻新病害叶鞘黑斑病的病原鉴定. 植物病理学报, 2014,44(3):225-231. |
HUANG W, XIA H, GAO B D . Identification of the causal agent of a new rice disease sheath black spot. Acta Phytopathologica Sinica , 2014,44(3):225-231. (in Chinese) | |
[32] | 金敏忠 .弯孢菌引起的变色米初步研究. 植物病理学报, 1989,19(1):21-26. |
JIN M Z . A preliminary study on discolored rice grains caused byCurvularia . Acta Phytopathologica Sinica , 1989,19(1):21-26. (in Chinese) | |
[33] |
江明, 黄俊斌 , HSIANG T, 郑莉. 杂交狗牙根叶枯病病原鉴定及其生物学特性. 植物保护学报, 2005,32(3):275-279.
doi: 10.3321/j.issn:0577-7518.2005.03.011 |
JIANG M, HAUNG J B, HSIANG T, ZHENG L . Identification and biological characteristics of Curvularia lunata causing leaf blight on Cynodon hybrid. Acta Phytophylacica Sinica , 2005,32(3):275-279. (in Chinese)
doi: 10.3321/j.issn:0577-7518.2005.03.011 |
|
[34] |
刘金平, 张新全, 刘朝辉 .几种禾草对新月弯孢菌的抗病能力. 江苏农业学报, 2005,21(3):216-220.
doi: 10.3969/j.issn.1000-4440.2005.03.015 |
LIU J P, ZHANG X Q, LIU Z H . Resistance of several Gram ineae grass species to Curvularia lunata . Jiangsu Journal of Agricultural Sciences , 2005,21(3):216-220. (in Chinese)
doi: 10.3969/j.issn.1000-4440.2005.03.015 |
|
[35] |
习平根, 李梅辉, 吴仕豪, 曹俐, 薛硕, 姜子德 .狗牙根草坪草真菌病害的病原菌鉴定. 华南农业大学学报, 2005,26(2):31-34.
doi: 10.3969/j.issn.1001-411X.2005.02.008 |
XI P G, LI M H, WU S H, CAO L, XUE S, JIANG Z D . Identification of the pathogenic fungi on Cynodon dactylon . Journal of South China Agricultural University , 2005,26(2):31-34. (in Chinese)
doi: 10.3969/j.issn.1001-411X.2005.02.008 |
|
[36] |
李春杰, 南志标, 崔嵩, 胡玉骄, 李东明, 李强, 李悦, 李舟 .几种真菌对3种常见冷季型草坪草的致病性测定. 草业科学, 2003,20(12):75-77.
doi: 10.3969/j.issn.1001-0629.2003.12.021 |
LI C J, NAN Z B, CUI S, HU Y J, LI D M, LI Q, LI Y, LI Z . Pathogenicity of ten fungi affecting three common cool-season turfgrasses of China. Pratacultural Science , 2003,20(12):75-77. (in Chinese)
doi: 10.3969/j.issn.1001-0629.2003.12.021 |
|
[37] | 翁启勇, 王青松, 何玉仙, 林敏, 余德亿 .福建草坪草病害初报. 草业学报, 1997,6(2):70-73. |
WENG Q Y, WANG Q S, HE Y X, LIN M, YU D Y . The occurrence of turf diseases in Fujian Province. Acta Prataculturae Sinica , 1997,6(2):70-73. (in Chinese) | |
[38] |
张亚玲, 王宝玉, 台莲梅, 左豫虎, 郑雯, 邓本良, 靳学慧 .黑龙江省和吉林省稻瘟病菌种群多样性研究. 黑龙江八一农垦大学学报, 2014,26(1):1-4, 31.
doi: 10.3969/j.issn.1002-2090.2014.01.001 |
ZHANG Y L, WANG B Y, TAI L M, ZUO Y H, ZHENG W, DENG B L, JIN X H . Study of population structure of Magnaporthe oryzae from rice in Heilongjiang and Jilin. Journal of Heilongjiang Bayi Agricultural University , 2014,26(1):1-4, 31. (in Chinese)
doi: 10.3969/j.issn.1002-2090.2014.01.001 |
|
[39] | DUAN X, TELLIER A, WAN A, LECONTE M , DE VALLAVIEILLE- OPE C, ENJALBERT[J]. Puccinia striiformis f. sp. tritici presents high diversity and recombination in the over-summering zone of Gansu, China. Mycologia , 2010,102(1):44-53. |
[40] | 刘二明 .水稻品种与稻瘟病菌的遗传多样性及稻瘟病持续控制研究[D].雅安: 四川农业大学, 2001. |
LIU E M .Genetic diversity of both rice varieties and blast pathogen, Magnaporthe grisea and sustainable control of disease[D].Yaan: Sichuan Agricultural University, 2001. ( in Chinese) | |
[41] |
ORDOÑEZ M E, KOLMER J A . Simple sequence repeat diversity of a worldwide collection of Puccinia triticina from durum wheat.Phytopathology, 2007,97(5):574-583.
doi: 10.1094/PHYTO-97-5-0574 pmid: 18943576 |
[42] |
TROCH V, AUDENAERT K, BEKAERT B HÖFTE M,HAESAERT G,. Phylogeography and virulence structure of the powdery mildew population on its ‘new’ host triticale. BMC Evolutionary Biology, 2012,12:76.
doi: 10.1186/1471-2148-12-76 pmid: 3457899 |
[43] | 郭建国, 杨凤珍, 杜蕙, 金社林, 张新瑞 .甘肃省玉米大斑病菌生理小种结构组成与交配型测定. 植物保护, 2018,44(6):85-90, 106. |
GUO J G, YANG F Z, DU H, JIN S L, ZHANG X R . Structural composition of physiological races and mating types of Setosphaeria turcica in Gansu.Plant Protection , 2018,44(6):85-90, 106. (in Chinese) | |
[44] | KNOX-DAVIES P S, DICKSON J G . Cytology of Helminthosporium turcicum and its ascigerous stage, Trichometasphaeria turcica . American Journal of Botany , 1960,47(5):328-339. |
[45] |
安鑫龙, 郑晓莲, 董金皋 .玉米大斑病长蠕孢生理小种的遗传变异. 微生物学通报, 2002,29(2):53-56.
doi: 10.3969/j.issn.0253-2654.2002.02.015 |
AN X L, ZHENG X L, DONG J G . Genetic variation of physiological races of Helminthosporium of maize leaf spot. Microbiology , 2002,29(2):53-56. (in Chinese)
doi: 10.3969/j.issn.0253-2654.2002.02.015 |
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|