中国农业科学 ›› 2011, Vol. 44 ›› Issue (22): 4560-4569.doi: 10.3864/j.issn.0578-1752.2011.22.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆二粒荚库容含量的多年QTL分析

杨振, 沈岩茹, 韩冬伟, 刘春燕, 蒋洪蔚, 陈庆山, 胡国华   

  1. 1.东北农业大学农学院,哈尔滨 150030
    2.黑龙江省农垦科研育种中心,哈尔滨 150090
    3.国家大豆工程技术研究中心,哈尔滨 150050
    4.黑龙江省农业科学院齐齐哈尔分院,黑龙江齐齐哈尔 161041
  • 收稿日期:2011-05-06 出版日期:2011-11-15 发布日期:2011-07-14
  • 通讯作者: 通信作者胡国华,Tel:0451-55199475;E-mail:hugh757@vip.163.com。陈庆山,Tel:0451-55191945;E-mail:qshchen@126.com
  • 作者简介:杨 振,Tel:0451-55191945;E-mail:yangzhen0467@163.com
  • 基金资助:

    国家现代农业产业技术体系建设专项资金(CARS-04-02A)、国家公益性行业(农业)科研专项(200903003)、国家转基因专项(2009ZX08009-013B)

QTL Analysis of Two-Seed Pod Storage Capacity in Soybean in Different Years

 YANG  Zhen, SHEN  Yan-Ru, HAN  Dong-Wei, LIU  Chun-Yan, JIANG  Hong-Wei, CHEN  Qing-Shan, HU  Guo-Hua   

  1. 1.东北农业大学农学院,哈尔滨 150030
    2.黑龙江省农垦科研育种中心,哈尔滨 150090
    3.国家大豆工程技术研究中心,哈尔滨 150050
    4.黑龙江省农业科学院齐齐哈尔分院,黑龙江齐齐哈尔 161041
  • Received:2011-05-06 Online:2011-11-15 Published:2011-07-14

摘要: 【目的】定位大豆二粒荚长、宽QTL,培育二粒荚高库容含量的品种,稳定或提高大豆的产量。【方法】以美国大豆品种Charleston为母本、东北农业大学大豆品系东农594为父本及其F2:14-F2:18代的重组自交系的147个株系为试验材料,164个SSR引物经亲本筛选后用于群体扩增,并构建遗传图谱。利用前两年1个地点和后三年2个地点的调查数据对亲本二粒荚长、宽性状进行调查及QTL分析。【结果】采用WinQTL Cartographer V2.0软件的CIM和MIM分析方法对QTL检测结果表明,多年多点的种植环境下,共检测到19个二粒荚长QTL分别位于A1、B2、C2、D1a、D1b、N和G连锁群上,检测到17个二粒荚宽QTL分别位于A1、C2、D1a、D1b、N和H连锁群上。在得到的这些QTL中,2种算法都能检测到的包括7个二粒荚长QTL,其连锁标记包括Satt200—qTSPL-a1-1—Satt042、Sat_214—qTSPL-d1a-1—Sat_112、Satt198—qTSPL-d1a-3—Satt502、Satt370—qTSPL-d1a-6—Satt402、Sat_092—qTSPL-c2-4—Satt289、Satt277—qTSPL-c2-5—Sct_188和Satt168—qTSPL-b2-1—Sat_083;1个二粒荚宽QTL,其连锁标记为Satt528—qTSPW-d1a-2—Satt182。在2年以上能被检测到包括8个二粒荚长QTL,其连锁标记为Satt200—qTSPL-a1-1—Satt042、Sat_119—qTSPL-a1-2—Sat_105、Sat_214—qTSPL-d1a-1—Sat_112、Satt220—qTSPL-d1a-4—Sat_162、Satt370—qTSPL-d1a-6—Satt402、Satt168—qTSPL-b2-1—Sat_083、Sat_092—qTSPL-c2-4—Satt289和Satt277—qTSPL-c2-5—Sct_188;4个二粒荚宽QTL,其连锁标记为Satt076—qTSPW-c2-1—Satt072、Satt335—qTSPW-c2-2—Sat_120、Satt200—qTSPW-a1-1—Satt042和Satt182—qTSPW-d1a-3—Satt584。【结论】得到不同方法和不同年份重复检测率较高的二粒荚长QTL和二粒荚宽QTL的连锁分子标记,为大豆二粒荚长、宽QTL的定位和今后改良大豆产量潜力提供了有力依据。

关键词: 大豆, 二粒荚长, 二粒荚宽, QTL分析

Abstract: 【Objective】The objective of this study is to locate consensus QTLs of two-seed pod length and width for breeding new varieties with high two-seed pod storage capacity and increase yield of soybean. 【Method】In order to find out the steady and repeatable QTLs of these traits, a F2:14-F2:18 RIL population containing 147 lines derived from a cross between Charleston as female and Dongnong594 as male parent were used in this experiment. A genetic linkage map was constructed with 164 SSR primers screened in two parents and amplified in 147 lines population. Two-seed pod length and width showing polymorphic in parents were investigated and QTLs were analyzed in one site in the early two years and two sites in the late three years. 【Result】 Nineteen QTLs for the two-seed pod length and width were analyzed with software Windows QTL Cartographer V2.0 by CIM and MIM under different environments, pod length were mapped in the linkage groups A1, B2, C2, D1a, D1b, N and G, 17 QTLs for two-seed pod width were mapped in the linkage groups A1, C2, D1a, D1b, N and H. In these QTLs,7 QTLs for two-seed pod length with linkage primers including Satt200—qTSPL-a1-1—Satt042, Sat_214—qTSPL-d1a-1—Sat_112, Satt198—qTSPL-d1a-3—Satt502, Satt370— qTSPL-d1a-6—Satt402, Sat_092—qTSPL-c2-4—Satt289, Satt277—qTSPL-c2-5—Sct_188 and Satt168—qTSPL-b2-1—Sat_083 could be detected together by CIM and MIM, 1 QTL for two-seed pod width including Satt528—qTSPW-d1a-2—Satt182 could be detected together by two methods. Eight two-seed pod length QTLs including Satt200—qTSPL-a1-1—Satt042, Sat_119— qTSPL-a1-2—Sat_105, Sat_214—qTSPL-d1a-1—Sat_112, Satt220—qTSPL-d1a-4—Sat_162, Satt370—qTSPL-d1a-6—Satt402, Satt168—qTSPL-b2-1—Sat_083, Sat_092—qTSPL-c2-4—Satt289 and Satt277—qTSPL-c2-5—Sct_188 could be detected in more than two years, 4 two-seed pod width QTLs including Satt076—qTSPW-c2-1—Satt072, Satt335—qTSPW-c2-2—Sat_120, Satt200— qTSPW-a1-1—Satt042 and Satt182—qTSPW-d1a-3—Satt584 could be detected in more than two years.【Conclusion】Mapping two-seed pod length and width QTL provide valuable information for soybean molecular marker assistant breeding selection.

Key words: soybean, two-seed pod length, two-seed pod width, QTL analysis