寄生柑橘和杉木的柑橘半穿刺线虫种内群体变异

doi:10.3864/j.issn.0578-1752.2011.09.009

摘要/Abstract

摘要： 【目的】对寄生于柑橘与杉木2种寄主的柑橘半穿刺线虫种群的rDNA-ITS区（ITS1-5.8S-ITS2）及形态变异进行研究，分析种群间遗传多态性及其亲缘关系。【方法】对采自中国3个省份（福建、四川、贵州）柑橘产区的21个柑橘半穿刺线虫种群（柑橘种群）及福建省杉木林区的6个柑橘半穿刺线虫种群（杉木种群）的ITS区进行PCR扩增、克隆与测序，通过相关软件进行序列比对与系统发育分析；对3个柑橘种群及1个杉木种群进行形态特征测计分析。【结果】柑橘半穿刺线虫的柑橘种群与杉木种群相比，ITS1序列相似性为93.2%—99.3%，ITS2序列相似性为92.4%—100%；基于ITS序列构建的系统发育树，所有杉木种群与福建省部分柑橘种群处于同一进化主分枝。4个柑橘半穿刺线虫种群间部分测量值存在显著差异，补充描述了种群内二龄幼虫尾部形态变异类型。【结论】柑橘半穿刺线虫柑橘种群和杉木种群在rDNA-ITS序列相似性高，序列差异不能用于种群的区分；杉木种群与福建部分柑橘种群在进化上可能有相同的地理起源。种群间部分测量值的显著差异及二龄幼虫尾部变异应属于种群正常变异。

关键词: 柑橘 , 杉木 , 柑橘半穿刺线虫 , ITS , 形态测量 , 种内群体变异

Abstract: 【Objective】 The objective of this study is to examine the variation in morphology and rDNA-ITS region (ITS1- 5.8S-ITS2) of Tylenchulus semipenetrans on citrus or Chinese fir (Cunninghamia lanceolata), and to analyze genetic variation and phylogenetic relationships among populations. 【Method】The ITS regions of 21 populations of T. semipenetrans collected from citrus groves in Fujian, Sichuan, and Guizhou provinces of China and 6 populations of T. semipenetrans collected from Chinese fir plantations in Fujian province were amplified, cloned and sequenced. Sequence alignment and phylogenetic analysis were performed by using related softwares. Morphometric characters were analyzed on 3 populations on citrus and one population on Chinese fir.【Result】 Comparison with populations on citrus and populations on Chinese fir, sequence similarities of ITS1 were 93.2%-99.3%, sequence similarities of ITS2 were 92.4%-100%. Six populations on Chinese fir and some populations on citrus collected from Fujian province gathered at the same main branch of phylogenetic tree based on the sequences of ITS1-5.8S-ITS2 of 27 populations using NJ method. The significant differences in some morphometric characters were found among 4 populations of T. semipenetrans. New morphological variations in the tail of 2nd stage juvenile in population were described. 【Conclusion】The high sequence similarity of ITS region of populations of T. semipenetrans on citrus or Chinese fir were demonstrated. Sequence variations of ITS region couldn’t be distinguished different populations of T. semipenetrans. The populations of T. semipenetrans on Chinese fir may have same biogeographic origin with some populations of T. semipenetrans on citrus in Fujian province. The difference in some morphometric characters and morphological variations in the tail of 2nd stage juvenile among populations may be normal variations.

Key words: citrus , Chinese fir , Tylenchulus semipenetrans , ITS , morphometry , intraspecific variability

刘国坤1;2;陈娟1;肖顺1;潘东明2;张绍升1. 寄生柑橘和杉木的柑橘半穿刺线虫种内群体变异[J]. 中国农业科学, 2011, 44(9): 1830-1836.

LIU Guo-kun; CHEN Juan; XIAO Shun; PAN Dong-ming; ZHANG Shao-sheng. Intraspecific Variability of Tylenchulus semipenetrans Populations on Citrus and Chinese Fir[J]. Scientia Agricultura Sinica, 2011, 44(9): 1830-1836.

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