中国农业科学 ›› 2010, Vol. 43 ›› Issue (22): 4754-4760 .doi: 10.3864/j.issn.0578-1752.2010.22.025

• 研究简报 • 上一篇    下一篇

组合生防菌与解盐促生菌复配对棉花的促生效应

赵雅峰,陶晶,武占省,刘燕,李春

  

  1. (石河子大学农学院/新疆兵团化工绿色过程重点实验室)
  • 收稿日期:2010-03-17 修回日期:2010-05-26 出版日期:2010-11-15 发布日期:2010-11-15
  • 通讯作者: 李春

Effects of Combination of Biocontrol Bacteria Complex with Salt Stress-Relieving Strain on Promotion of Cotton Growth

ZHAO Ya-feng, TAO Jing, WU Zhan-sheng, LIU Yan, LI Chun   

  1. (石河子大学农学院/新疆兵团化工绿色过程重点实验室)
  • Received:2010-03-17 Revised:2010-05-26 Online:2010-11-15 Published:2010-11-15
  • Contact: LI Chun

摘要:

【目的】研究多功能菌剂BCL-Rs对盐胁迫和立枯丝核菌(Rhizoctonia solani)危害下棉花生长的影响。【方法】组合生防菌枯草芽孢杆菌(Bacillus subtilis)BCL-8与解盐促生菌产酸克雷伯氏菌(Klebsiella oxytoca)Rs-5复配获得BCL-Rs,通过盆栽、小区和大田试验对BCL-Rs促进棉花生长的功能进行验证。【结果】BCL-Rs对立枯丝核菌有很好的抑菌活性,并且能够有效提高棉花的耐盐性。盆栽试验和小区试验结果表明,BCL-Rs对棉花立枯病的防治效果分别达到了55.37%和51.06%,在盐胁迫条件下使棉苗干重分别提高了40.85%和25.81%,具有显著的防病促生和缓解盐胁迫的效能。棉花大田试验结果表明,BCL-Rs包衣处理及滴灌处理对棉花苗期生长有明显的促进作用,可显著提高棉花植株的鲜重、干重和株高,增大植株的叶面积,较常规处理增产10.51%。【结论】在BCL-8的防病性能与Rs-5的解盐促生功能的协同效应下,BCL-Rs能够更有效地防治盐胁迫条件下的棉花立枯丝核菌造成的危害。

关键词: 棉花, 多功能菌剂, 枯草芽孢杆菌, 立枯丝核菌, 解盐

Abstract:

【Objective】 Effects of multi-functional bacterial mixture BCL-Rs on promotion of cotton growth under salt stress and Rhizoctonia solani damping-off were studied. 【Method】 BCL-Rs were obtained by combining biocontrol bacteria complex BCL-8 with salt stress-relieving strain Klebsiella oxytoca Rs-5. The function of BCL-Rs on cotton growth was investigated in pot tests, plot experiments and field trials. 【Result】 The BCL-Rs had an obvious inhibitory activity to Rhizoctonia solani, and BCL-Rs-treated cottons showed a good performance of relieving salt stress, and significant resistance to damping-off caused by Rhizoctonia solani. Compared with the control, the control efficacy of damping-off on cotton reached up to 55.37% and 51.06% in pot and plot experiments, respectively. And the dry weight of cotton seedlings increased by 40.85% and 25.81% under the salt stress conditions, respectively. The results of cotton field trials showed that the growth of cotton seedlings was promoted by coating and drip irrigation with BCL-Rs. Physiological characters of cotton, including plant fresh weight, dry weight, plant height, and leaf area increased significantly. The production of cotton increased by 10.51% compared with the conventional treatment. 【Conclusion】Cotton damping-off caused by Rhizoctonia solani was significantly suppressed by the bacterial mixture BCL-Rs even under the salt stress conditions, indicating a synergistic effect of biocontrol bacteria BCL-8 and salt stress-relieving strain Rs-5.

Key words: cotton, multi-microbial agent, Bacillus subtilis BCL-8, Rhizoctonia solani, salt stress-relieving