关键词: 白菜')">白菜, 叶缘裂刻, cDNA-AFLP, 差异表达, SCAR 标记

Abstract:

【Objective】 The objective of the research was to detect the cDNA fragments of the genes related to leaf-lobed phenotype in no-heading Chinese cabbage and study its molecular mechanism. 【Method】 The study was carried out to reveal the differential expression of genes in the near-isogenic lines with lobed leaves by cDNA amplified fragments length polymorphism (cDNA-AFLP) analysis. 【Result】 There were 57 differentially expressed bands between two lines which belonged to 55 unique genes. Among them, 25 bands were associated with the NIL lines of smooth margin, while 30 bands with the NIL lines of lobed margins. Fifty-four bands had the homologous sequences in NCBI database, and only 1 band showed an unknown sequence. The genes with these homologous sequences are involved in the following biological process: metabolism, transcription, cell organization, protein synthesis and degradation, signal transduction mechanism, photosystemⅠ/Ⅱ and retrotransposon factor, etc. Furthermore, the result of AFLP-SCAR analysis was consistent with the result of cDNA-AFLP analysis. 【Conclusion】 Gene expression profiling of the near-isogenic lines with smooth and lobed leaf margins from no-heading Chinese cabbage were revealed and identified via cDNA- AFLP method, and some genes related to development of lobed leaves were proposed. The AFLP-SCAR markers are helpful for marker-assisted selection of agronomic traits with lobed leaves.

Key words: no-heading Chinese cabbage')">no-heading Chinese cabbage, lobed leaves, cDNA-AFLP, differential expression, SCAR marker