中国农业科学 ›› 2010, Vol. 43 ›› Issue (9): 1883-1894 .doi: 10.3864/j.issn.0578-1752.2010.09.015

• 园艺 • 上一篇    下一篇

西瓜与枯萎病菌非亲和互作的表达序列标签分析

吕桂云,郭绍贵,张海英,耿丽华,许勇

  

  1. (中国农业科学院蔬菜花卉研究所)

  • 收稿日期:2009-09-18 修回日期:2009-11-09 出版日期:2010-05-01 发布日期:2010-05-01
  • 通讯作者: 许勇

Analysis of Expressed Sequence Tags in the Incompatible Interaction Between Watermelon and Fusarium oxysporum

LÜ, Gui-yun, GUO Shao-gui, ZHANG Hai-ying, GENG Li-hua, XU Yong

  

  1. (中国农业科学院蔬菜花卉研究所)

  • Received:2009-09-18 Revised:2009-11-09 Online:2010-05-01 Published:2010-05-01
  • Contact: XU Yong

摘要:

【目的】从整体水平上阐明西瓜与枯萎病菌非亲和互作基因表达的特征。【方法】用SSH方法构建枯萎病菌接种处理后不同时间点的cDNA文库,对其中获得3 895条高质量的表达序列标签(EST)序列进行分析。【结果】在测定4 431条cDNA序列中,序列拼接后获得1 756条unigene,经过注释和分析,发现可能参与西瓜与枯萎病菌非亲和互作的转录因子、激酶和防卫基因,及茉莉酸和木质素生物合成途径等。【结论】 在西瓜与枯萎病菌互作的SSH-cDNA文库中,与抗病防御相关基因约占36.3%,初步了解了西瓜与枯萎病菌非亲和互作过程中基因表达信息,这些基因的发现为在分子水平上研究西瓜与枯萎病菌的互作机制以及相关重要基因的克隆与功能分析奠定基础。

关键词: 西瓜, 枯萎病菌, cDNA 文库, 表达序列标签(EST)

Abstract:

【Objective】The objective of the experiment is to study the mechanisms of watermelon resistance to Fusarium oxysporum by investigating the expression profiles of the related genes in the incompatible interaction. 【Method】 A cDNA library of the watermelon root tissues induced by Fusarium oxysporum f. sp. niveurn was constructed using the SMART protocol. The positive clones of the cDNA library were sequenced randomly and 3 895 desired ESTs were obtained for bioinformatics analysis. 【Result】 Total 1 756 unique sequences were annotated for the functions. Transcription factors, protein kinase, defence gene, shikimate-phenylpropanoid-lignin metabolism and JA biosynthesis were found in the incompatible interaction between watermelon and Fusarium oxysporum. 【Conclusion】 There were 36.3% of the annotated unique sequences had similar function with disease resistance and defense. This information showed us an elementary profile of the interaction mechanism between watermelon and Fusarium oxysporum. These results developed a good foundation for the further exploration of the interaction mechanism at the molecular level and the related important genes cloning.

Key words: Citrullus lanatus, Fusarium oxysporum, cDNA library, expressed sequence tags (EST)