关键词: 冷应激, HSP70, 表达, 荧光定量PCR, 鹅

Abstract:

【Objective】 The objective of this study was to analyze the expression of HSP70 mRNA in Hypothalamic-Pituitary- Thyroid (HPT) axis under cold stress (12±1℃). 【Method】 The dynamic expression of HSP70 mRNA in HPT axis of 7 d Wanxi White goose was got utilizing real-time fluorescent quantification reverse transcriptase PCR (FQ-RT-PCR). 【Result】 Hypothalamic HSP70 mRNA transcription level went up significantly at1.5, 6, and 9 h of cold stress; It decreased to normal level in the other times. It increased again after cold exposure for 6 hours, moreover, it went up to the highest level at 6 h. Hereafter it declined to normal level. Pituitary HSP70 mRNA transcription level descended significantly at 0.5, 1.5, 6, and 12 h . It went up again gradually to normal level in the next 12 hours. It decreased again significantly at 0.5, 3, 6 h after cold exposure, hereafter gradually increased to normal level. Thyroid HSP70 mRNA transcription level went up quickly at 0.5h, hereafter decreased at 3, 6, 12 h significantly under cold stress. It went up significantly at 0.5h and 3h and it was down-regulated gradually to normal level in the other times after cold stress. 【Conclusion】 The expression rule of HSP70 mRNA was different in Hypothalamic-Pituitary-Thyroid (HPT) axis. Hypothalamic HSP70 mRNA transcription level was the highest which showed going up trend as a whole. HSP70 was the up-regulation gene of hypothalamic in cold stress reaction. Pituitary and thyroid HSP70 mRNA transcription level was repressed which showed that it was the down-regulation gene of pituitary and thyroid in cold stress reaction.

Key words: cold stress, HSP70 gene, expression, real time PCR, goose