关键词: 茄子, 果色, 花青素, 混合品系法, AFLP标记, SCAR标记

Abstract:

【Objective】 The study identified the molecular marker linked to the gene which controlled fruit color (fc) or fruit anthocyanin biosynthesis in eggplant, and tested the application of bulked line analysis in identifying molecular markers. 【Method】With the method of assessing individuals and bulked line analysis, the AFLP technique of DNA analysis was employed to screen the markers linked to gene of interest among 136 accessions including 130 Solanum melongena and 6 relatives. 【Result】 One codominant AFLP marker was identified to be related to the peel color of black purple and purplish red after screening 58 advanced inbred lines individually. Sequencing of the polymorphic fragment indicated that their lengths were 107 bp and 108 bp, respectively, and they were orthologous sequences with an insertion /deletion of only one nucleiotide. The AFLP marker was further converted into SCAR marker. The analysis on another set of 78 accessions, combining with previous data, indicated that the SCAR marker associated significantly with the black purple and purplish color of fruit peel, but with no correlation with other colors. Beside the above marker, another 6 markers showing polymorphic between black purple fruit pool and purplish red ones were also detected using bulked line analysis, and all of them were demonstrated to be highly correlated to the fruit purple color of eggplant as the SCAR marker when tested on the individual lines. 【Conclusion】 The generated SCAR markers and AFLP markers can be used in molecular marker-assisted breeding for fruit peel color in eggplant.

Key words: eggplant (Solanum melongena L.), peel color, anthocyanin, bulked line analysis, AFLP, SCAR