中国农业科学 ›› 2009, Vol. 42 ›› Issue (10): 3397-3404 .doi: 10.3864/j.issn.0578-1752.2009.10.003

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用SSR标记探讨骨干亲本欧柔在衍生品种的遗传

李小军,徐 鑫,刘伟华,李秀全,李立会   

  1. (中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程)
  • 收稿日期:2009-01-04 修回日期:2009-04-07 出版日期:2009-10-10 发布日期:2009-10-10
  • 通讯作者: 李立会

Genetic Diversity of the Founder Parent Orofen and Its Progenies Revealed by SSR Markers

LI Xiao-jun, XU Xin, LIU Wei-hua, LI Xiu-quan, LI Li-hui
  

  1. (中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程)
  • Received:2009-01-04 Revised:2009-04-07 Online:2009-10-10 Published:2009-10-10
  • Contact: LI Li-hui

摘要:

【目的】研究骨干亲本欧柔染色体片段在衍生后代的分布及所关联的性状,旨在揭示骨干亲本对后代品种的遗传贡献。【方法】对小麦骨干亲本欧柔及其衍生的23份在中国生产和育种中发挥重要作用的后代品种进行农艺性状调查和SSR扫描。【结果】在43个SSR位点欧柔特异等位变异在一代品种的传递频率高于理论比例0.38; 在41个位点欧柔特异等位变异在二代品种的传递频率高于理论比例0.18;在21个位点欧柔特异等位变异被持续选择(在2个子代的分布频率分别大于0.38和0.18)。SSR标记与农艺性状关联分析表明,在Xwmc710、Xbarc235和Xbarc252位点,欧柔等位变异类型在后代具有高的分布频率且与较高的穗粒数、产量相关。【结论】推测本研究发现的重要染色体区域及相关的优异性状在中国小麦品种改良中发挥了重要作用,是进一步研究的重点。

关键词: 小麦, 骨干亲本, 染色体位点, 选择, 关联分析

Abstract:

【Objective】 The main objective is to uncover the genetic contribution of founder parent to its offspring through tracking chromosomal regions of Orofen in its progeny. 【Method】 Orofen and its 23 representative progenies were analyzed using morphological traits in five environments and microsatellite markers. 【Result】 The frequencies of the alleles which were amplified in Orofen but absent in other 4 parental accessions (Jinan 4, Beijing 8, 54405 and Heine Hvede) at 43 SSR loci were higher than the theoretical ratio 0.38 in the first generation; the frequencies of the alleles which were amplified in Orofen but absent in other 6 parental accessions (Jinan 4, Beijing 8, 54405, Heine Hvede, Lovrin 10 and Lovrin 18) at 41 SSR loci were higher than the theoretical ratio 0.18 in the second generation; at twenty-one of the found loci above, the unique alleles amplified only in Orofen have been selected continuously in the both generations. Association mapping indicated that the alleles according to Orofen which occurred in most progenies at the loci Xwmc710, Xbarc235 and Xbarc252 were associated with higher grain number and yield when compared with the remaining amplified alleles at the same loci. 【Conclusion】 It was speculated that the genomic region detected and associated traits played an important role for the improvement of wheat in China and could be acted as the emphases for further study.

Key words: wheat, founder parent, chromosomal loci, selection, association mapping