关键词: hrf2基因, 农杆菌介导转化法, 转基因油菜, 抗病性

Abstract: 【Objective】The objective of this study was to increase the resistance of rape using the method of transformation.【Method】The gene hrf2 derived from Xanthomonas oryzae pv. oryzicola encoding harpinXooc protein has been constructed into transgenic vector pCAMBIA1301.The cotyledonal petiole segments from rapeseed variety Yangyou 4 were infected and transformed by Agrobacterium tumefaciens strain LBA4404/ pCAMBIA1301-hrf2.【Result】Hygromycin-resistant green shoots were obtained. PCR，PCR-Southern，RT-PCR and GUS analysis have confirmed successful integration of the foreign gene into the genomes of the rapeseed variety Yangyou 4. Disease bioassays of transgenic plants indicated that the resistance of transgenic plants to Rape sclerotiniose.【Conclusion】The hrf2 gene can be transferred into rape using the method of Agrobacterium-mediated transformation, which increased the resistance to Sclerotinia Sclerotinorium in the transgenic plant.