中国农业科学 ›› 2008, Vol. 41 ›› Issue (5): 1536-1541 .doi: 10.3864/j.issn.0578-1752.2008.05.038

• 研究简报 • 上一篇    下一篇

龙麦20小麦品种中7+8*亚基和17+18亚基近等基因系间的品质差异

张延滨,赵海滨,宋庆杰,于海洋,张春利,辛文利,肖志敏   

  1. 黑龙江省农业科学院作物育种研究所
  • 收稿日期:2006-01-25 修回日期:2006-05-16 出版日期:2008-05-10 发布日期:2008-05-10

Quality Difference of Near-isogenic Lines of HMW Glutenin Subunits

  

  1. 黑龙江省农业科学院作物育种研究所
  • Received:2006-01-25 Revised:2006-05-16 Online:2008-05-10 Published:2008-05-10

摘要: 【目的】确定高分子麦谷蛋白亚基17+18和7+8*间的遗传差异。【方法】利用生化标记和选择性回交(5次)的方法将拥有超量表达的Bx7亚基的加拿大超强筋小麦品种Glenlea的7+8*亚基转移到了小麦品种龙麦20亚基组成为1,17+18,5+10的近等基因系(NILs)中,获得了龙麦20的17+18亚基和7+8*亚基的近等基因系。2005年这对近等基因系被种植在黑龙江农业科学院育种研究所的试验地里,试验设计采用随机取组,6次重复。【结果】品质分析结果表明,7+8*亚基类型的龙麦20与17+18亚基龙麦20类型相比,面粉蛋白质含量提高5%(P =0.012)、干面筋含量增加4%(P =0.018)、湿面筋/干面筋降低2%(P =0.043)、沉降值提高10%(P =0.013)、形成时间提高13%(P=0.258)、稳定时间提高256%(P =0.029)、断裂时间提高86%(P =0.020)、软化度降低35%(P =0.007)。【结论】7+8*亚基对面筋强度有较大的正向影响。

关键词: 小麦, 品质, 高分子量麦谷蛋白亚基, 7+8*亚基, 近等基因系

Abstract: High molecular weigh glutenin subunits (HMW-GS) 7+8* was introduced into Longmai 20(1,17+18,5+10) by 5 consecutive backcrosses with biochemical marker assisted selection from a Canadian extra strong wheat cultivar Glenlea which possessing an overexpression of the Bx7 subunit. The near isogenic lines (NILs) of HMW-GS were obtained and grown in the experimental field of Crop Breeding Institute in 2005. The experimental design was a randomized block with six replicates. As compared to NILs with 17+18 subunit, the average of quality data of NILs with 7+8* subunit were 5 %(P =0.012)、4%(P =0.018)、10% (P =0.013)、13%(P=0.258)、256%(P =0.029) and 86%(P =0.020) higher in flour protein content、dry gluten content、Zeleny sedimentation、development time、stability and breakdown time, respectively, and were 2%(P =0.033)and 35%(P=0.007)lower in wet gluten/dry gluten and degree of softening, respectively. The application of subunits 7+8* in wheat breeding for quality was discussed in this study.

Key words: Wheat, Quality, HMW-GS, 7+8* subunits, Near-Isogenic Lines(NILs)