黄花蒿提取物对朱砂叶螨生物活性的研究

doi:10.3864/j.issn.0578-1752.2008.03.012

中国农业科学 ›› 2008, Vol. 41 ›› Issue (3): 720-726 .doi: 10.3864/j.issn.0578-1752.2008.03.012

黄花蒿提取物对朱砂叶螨生物活性的研究

张永强,丁伟,赵志模,吴静,樊钰虎

西南大学植物保护学院

收稿日期:2007-01-29 修回日期:1900-01-01 出版日期:2008-03-10 发布日期:2008-03-10
通讯作者: 丁伟

Temporal and Spatial Dynamic Studies on Acarcidal Bioactivities of Artemisia annua L. against Tetranychus cinnabarinus Bois. (Acari: Tetranychidae)

Yong-Qiang ZHANG Wei DING Zhi-mo ZHAO Jing WU Yu-Hu FAN

西南大学植物保护学院

Received:2007-01-29 Revised:1900-01-01 Online:2008-03-10 Published:2008-03-10
Contact: Wei DING

摘要/Abstract

摘要： 【目的】明确黄花蒿杀螨活性物质的最佳提取工艺；选择合适的溶剂和最佳的植株部位，明确黄花蒿杀螨活性物质的时间和空间分布状态，为综合开发利用黄花蒿提供科学依据。【方法】采集4、5、6、7和9月5个月份的黄花蒿植株，分成根、茎、叶3个部分，采用石油醚（30～60℃）、石油醚（60～90℃）、乙醇、丙酮和水溶剂的平行和顺序提取方法，共计获得135种提取物，用玻片毛细管法测定其对朱砂叶螨的杀螨活性。【结果】在杀螨活性方面，黄花蒿的杀螨活性随植株的生长呈增加的趋势，总体表现为7月份＞6月份＞5月份＞4月份，但9月份的杀螨活性与7月份相比有所下降。5个月份的黄花蒿叶丙酮平行提取物的活性都比其它提取物强，处理朱砂叶螨48 h的校正死亡率介于74%～100%之间。从对朱砂叶螨的致死时间动态上看，9月份、7月份、6月份、5月份、4月份黄花蒿叶的丙酮平行提取物对朱砂叶螨处理48 h，LC50分别为0.5986，0.4341，0.8376，0.9443和1.3817 mg•ml-1。对活性较高的7月份叶的丙酮提取物进行柱层析分离，得到13个组分，其中组份11和12的LC50分别为0.3683和0.1586 mg•ml-1，组分12的活性相对于七月份叶丙酮提取物而言提高了约5倍。【结论】黄花蒿7月份叶的丙酮提取物的毒力最好，5 mg•ml-1对朱砂叶螨处理48 h，校正死亡率为100%。7月份叶丙酮平行提取物柱层析组分11和12是活性组分，是进一步研究的重点。

关键词: 黄花蒿, 朱砂叶螨, 平行提取物, 生物活性

Abstract: 【Objective】This aim of this study was to determine the best extraction technique, the suitable solvent and the optimal plant parts and acaricidal activities temporal and spatial dynamic of Artemisia annua L.【Method】This paper dealt with the acarcidal bioactivities against Tetranychus cinnabarinus of total 135 extracts of petroleum ether 30-60℃, petroleum ether 60-90℃, ethanol, acetone and water parallel and sequenced extracts from the leaves, stems and roots of different period traditional Chinese medicine Artemisia annua L. in April, May, June, July and September 2006. All extracts were diluted to 5mg/ml, and examined their bioactivities against T. cinnabarinus in laboratory. 【Results】The results showed that at the concentration of 5mg/ml, the acaricidal bioactivities elevated as the development of A. annua plants, the general tendency exhibited July> June> May> April, but the September decreased comparing with the July. However, the most effective extracts in five months were all acetone parallel extract of A. annua leaf, and the corrected mortalities after 48h treatment were ranged from 74% to 100%. The median lethal concentrations (LC50) of acetone parallel extracts of A. annua leaves in September, July, June, May and April were 0.5986, 0.4341, 0.8376, 0.9443 and 1.3817mg/ml, respectively treated after 48h against T. cinnabarinus. The 13 groups were separated from acetone extracts of A. annua leaves in July by column chromatography, all of which the 11th and 12th groups exhibited strong activities. The median lethal concentrations of the 11th and 12th groups against T. cinnabarinus were 0.3683and 0.1586mg/ml, respectively. 【Conclusion】The acetone parallel extract of Artemisia annua leaf in July was most toxicity to T. cinnabarinus Bois. and the corrected mortality was 100% after exposure 48h. The No. 11 and No. 12 groups of acetone parallel extract was the most active components, could be acted as the emphases in further study.

Key words: Artemisia annua, Tetranychus cinnabarinus, Acetone parallel extract, bioactivities

张永强,丁伟,赵志模,吴静,樊钰虎. 黄花蒿提取物对朱砂叶螨生物活性的研究[J]. 中国农业科学, 2008, 41(3): 720-726 .

Yong-Qiang ZHANG Wei DING Zhi-mo ZHAO Jing WU Yu-Hu FAN. Temporal and Spatial Dynamic Studies on Acarcidal Bioactivities of Artemisia annua L. against Tetranychus cinnabarinus Bois. (Acari: Tetranychidae)[J]. Scientia Agricultura Sinica, 2008, 41(3): 720-726 .

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2008.03.012

https://www.chinaagrisci.com/CN/Y2008/V41/I3/720

[1]	李慧,尹士采,郭宗香,马好运,任梓齐,折冬梅,梅向东,宁君. 宽胫夜蛾性信息素类似物的合成及其生物活性[J]. 中国农业科学, 2022, 55(9): 1790-1799.
[2]	任梓齐,康玉洁,李海珍,王连刚,马好运,李慧,王留洋,梅向东,宁君. 陌夜蛾性信息素类似物的合成及其生物活性[J]. 中国农业科学, 2022, 55(23): 4640-4650.
[3]	刘泓,郭玉杰,许雄,李侠,张鸿儒,齐立伟,孙雪梅,张春晖. 不同畜禽骨蛋白肽的制备、理化特性表征及其生物活性[J]. 中国农业科学, 2022, 55(13): 2629-2642.
[4]	卞荣军,刘晓雨,郑聚锋,程琨,张旭辉,李恋卿,潘根兴. 生物质炭可溶性有机物化学组成及生物活性意义[J]. 中国农业科学, 2022, 55(11): 2174-2186.
[5]	王丽芳,张兴夫. 黄花蒿醇提物对奶牛乳腺细胞中共轭亚油酸合成相关酶基因表达的作用[J]. 中国农业科学, 2019, 52(18): 3271-3278.
[6]	刘小强，蒋红波，李慧敏，熊英，王进军. 赤拟谷盗章鱼胺受体3（TcOctβR3）cDNA克隆、表达及功能[J]. 中国农业科学, 2018, 51(7): 1315-1324.
[7]	申光茂，李恒，梁金辉，何林?. 朱砂叶螨TcGSTM7的体外表达及其功能[J]. 中国农业科学, 2018, 51(5): 905-913.
[8]	王丽芳,斯琴毕力格,敖长金. 黄花蒿提取物对奶牛瘤胃发酵指标的影响[J]. 中国农业科学, 2018, 51(23): 4548-4555.
[9]	敖已倩云，申光茂，王梦瑶，刘家路，潘宇，何林. 朱砂叶螨β-COP和Sro基因鉴定及其沉默致死效果[J]. 中国农业科学, 2017, 50(18): 3529-3539.
[10]	赵恒科，蓝月，南灿，胡月，饶萍，田亚，严伟，钱坤，何林. 8%甲氰菊酯·丁氟螨酯纳米乳剂的研制及其性能[J]. 中国农业科学, 2016, 49(14): 2700-2710.
[11]	罗楚平1, 2, 王晓宇1, 周华飞1, 2, 刘邮洲1, 陈志谊1, 2. 生防菌Bs916合成脂肽抗生素泛革素的操纵子结构功能及生物活性[J]. 中国农业科学, 2013, 46(24): 5142-5149.
[12]	罗金香, 丁伟, 张永强, 杨振国, 李阳, 丁丽娟. 双去甲氧基姜黄素及其N-甲基吡唑衍生物对朱砂叶螨生物活性和多种酶活性的影响[J]. 中国农业科学, 2013, 46(13): 2833-2844.
[13]	殷素侠, 廖美德, 徐汉虹. 光敏蛋白脂质体的制备与生物活性[J]. 中国农业科学, 2013, 46(1): 60-68.
[14]	方祖凯, 王勇, 李俊凯, 杜铁钢, 兰腾芳. N-苯氧苯基取代的α-氨基酸衍生物的合成及生物活性[J]. 中国农业科学, 2011, 44(24): 4999-5005.
[15]	罗楚平,王晓宇,陈志谊,刘永锋,张杰,刘邮洲,聂亚锋,余俊杰,尹小乐 . 枯草芽孢杆菌Bs916中脂肽抗生素Bacillomycin L的操纵子结构及生物活性 [J]. 中国农业科学, 2010, 43(22): 4624-4634 .

黄花蒿提取物对朱砂叶螨生物活性的研究

Temporal and Spatial Dynamic Studies on Acarcidal Bioactivities of Artemisia annua L. against Tetranychus cinnabarinus Bois. (Acari: Tetranychidae)

PDF

赞

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0