Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (10): 1996-2001 .

• PLANT PROTECTION • Previous Articles     Next Articles

EST-Markers Flanking the Rice Bacterial Blight Resistance Gene Xa23 and Their Application in Marker-Assisted Selection

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  1. 中国农业科学院作物科学研究所
  • Received:2005-02-06 Revised:1900-01-01 Online:2005-10-10 Published:2005-10-10

Abstract: A F2 population, with 2 562 individuals, of JG30/CBB23 was constructed for molecular mapping of the bacterial blight resistance gene Xa23 in rice. Inoculation test of the F2 plants showed that the segregation ratio of resistant and susceptible fits to 3:1. Based on the amthors previous mapping of Xa23 gene, 12 EST markers from Rice Genome Program (RGP) database were surveyed in the susceptible F2 plants and two markers, C189 and CP02662, were found flanking the Xa23 gene, with genetic distance of 0.8cM and 1.3cM, respectively. These two EST markers were successfully used in rice breeding program. The results showed that efficiency of C189 marker-assisted selection (MAS) for Xa23 gene was almost 100%. Three elite hybrid rice restore lines with Xa23 gene and improved bacterial blight resistance have been developed.

Key words: Rice, Xa23, Rice bacterial blight, EST, MAS

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