Horizontal gene transfer (HGT) has been proved a major driving force in prokaryotic evolution.  However, the molecular functions of these transferred genes in pathogenic bacteria especially plant pathogenic bacteria are still not fully investigated.  In this study, the whole-genome in silico analysis was performed and found a syringopeptin synthetase (syp) homolog in Burkholderia glumae, which can cause bacterial panicle blight in rice, was predicted to be horizontally transferred from Pseudomonas ancestor with solid confidence by phylogenetic analysis.  The comprehensive molecular experiments were performed to study the potential role of this gene in B. glumae.  Inoculation of rice panicles with the syp mutant resulted in 60% lower disease index compared with the wild type (WT) parent strain, suggesting the requirement of syp for the full virulence of B. glumae.  Chromatography analysis of exudates from B. glumae showed suppression of synthesis of metabolites analogous to syringopeptin in the mutants.  All these data raise the possibility of HGT phenomenon in shaping the virulence and adaptation of B. glumae over evolutionary time.

 

Potato (Solanum tuberosum L.) is the third most important food crop worldwide after wheat and rice in terms of human consumption.  A critical domestication trait for potato was the decrease of toxic steroidal glycoalkaloids (SGAs) in tuber flesh.  Here, we used a diploid F2 segregating population derived from a cross between S. tuberosum and the wild potato species Solanum chacoense to map the quantitative trait loci (QTLs) associated with the regulation of SGAs content in tuber flesh.  In a three-year study, we identified two QTLs on chromosomes 2 and 8 affecting SGAs content in tuber flesh.  The QTL on chromosome 8 harbors 38 genes that are co-expressed with the GLYCOALKALOID METABOLISM genes.  These findings lay the foundation for exploiting the genes controlling SGAs content in tuber flesh and they provide a theoretical basis for the use of wild germplasm in potato breeding.

 

Sowing date and seeding rate are critical for productivity of winter wheat (Triticum aestivum L.).  A three-year field experiment was conducted with three sowing dates (20 September (SD1), 1 October (SD2), and 10 October (SD3)) and three seeding rates (SR67.5, SR90, and SR112.5) to determine suitable sowing date and seeding rate for high wheat yield.  A large seasonal variation in accumulated temperature from sowing to winter dormancy was observed among three growing seasons.  Suitable sowing dates for strong seedlings before winter varied with the seasons, that was SD2 in 2012–2013, SD3 in 2013–2014, and SD2 as well as SD1 in 2014–2015.  Seasonal variation in precipitation during summer fallow also had substantial effects on soil water storage, and consequently influenced grain yield through soil water consumption from winter dormancy to maturity stages.  Lower consumption of soil water from winter dormancy to booting stages could make more water available for productive growth from anthesis to maturity stages, leading to higher grain yield.  SD2 combined with SR90 had the lowest soil water consumption from winter dormancy to booting stages in 2012–2013 and 2014–2015; while in 2013–2014, it was close to that with SR67.5 or SR112.5.  For productive growth from anthesis to maturity stages, SD2 with SR90 had the highest soil water consumption in all three seasons.  The highest water consumption in the productive growth period resulted in the best grain yield in both low and high rainfall years.  Ear number largely contributed to the seasonal variation in grain yield, while grain number per ear and 1 000-grain weight also contributed to grain yield, especially when soil water storage was high.  Our results indicate that sowing date and seeding rate affect grain yield through seedling development before winter and also affect soil water consumption in different growth periods.  By selecting the suitable sowing date (1 October) in combination with the proper seeding rate of 90 kg ha^–1, the best yield was achieved.  Based on these results, we recommend that the current sowing date be delayed from 22 or 23 September to 1 October.