|
|
|
|
|
| Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.) |
| XU Liang, JIANG Qiu-wei, WU Jian, WANG Yan, GONG Yi-qin, WANG Xian-li, Limera Cecilia , LIU Li-wang |
1、National Key Laboratory of Crop Genetics and Germplasm Enhancement/Engineering Research Center of Horticultural Crop Germplasm
Enhancement and Utilization, Ministry of Education/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China
2、Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
|
|
|
摘要 Downy mildew (DM), caused by the fungus Peronospora parasitica, is a destructive disease of radish (Raphanus sativus L.) worldwide. Host resistance has been considered as an attractive and environmentally friendly approach to control the disease. However, the genetic mechanisms of resistance in radish to the pathogen remain unknown. To determine the inheritance of resistance to DM, F1, F2 and BC1F1 populations derived from reciprocal crosses between a resistant line NAU-dhp08 and a susceptible line NAU-qtbjq-06 were evaluated for their responses to DM at seedling stage. All F1 hybrid plants showed high resistance to DM and maternal effect was not detected. The segregation for resistant to susceptible individuals statistically fitted a 3:1 ratio in two F2 populations (F2(SR) and F2(RS)), and 1:1 ratio in two BC1F1 populations, indicating that resistance to DM at seedling stage in radish was controlled by a single dominant locus designated as RsDmR. A total of 1 972 primer pairs (1036 SRAP, 628 RAPD, 126 RGA, 110 EST-SSR and 72 ISSR) were screened, and 36 were polymorphic between the resistant and susceptible bulks, and consequently used for genotyping individuals in the F2 population. Three markers (Em9/ga24370, NAUISSR826700 and Me7/em10400) linked to the RsDmR locus within a 10.0 cM distance were identified using bulked segregant analysis (BSA). The SRAP marker Em9/ga24370 was the most tightly linked one with a distance of 2.3 cM to RsDmR. These markers tightly linked to the RsDmR locus would facilitate marker-assisted selection and resistance gene pyramiding in radish breeding programs.
Abstract Downy mildew (DM), caused by the fungus Peronospora parasitica, is a destructive disease of radish (Raphanus sativus L.) worldwide. Host resistance has been considered as an attractive and environmentally friendly approach to control the disease. However, the genetic mechanisms of resistance in radish to the pathogen remain unknown. To determine the inheritance of resistance to DM, F1, F2 and BC1F1 populations derived from reciprocal crosses between a resistant line NAU-dhp08 and a susceptible line NAU-qtbjq-06 were evaluated for their responses to DM at seedling stage. All F1 hybrid plants showed high resistance to DM and maternal effect was not detected. The segregation for resistant to susceptible individuals statistically fitted a 3:1 ratio in two F2 populations (F2(SR) and F2(RS)), and 1:1 ratio in two BC1F1 populations, indicating that resistance to DM at seedling stage in radish was controlled by a single dominant locus designated as RsDmR. A total of 1 972 primer pairs (1036 SRAP, 628 RAPD, 126 RGA, 110 EST-SSR and 72 ISSR) were screened, and 36 were polymorphic between the resistant and susceptible bulks, and consequently used for genotyping individuals in the F2 population. Three markers (Em9/ga24370, NAUISSR826700 and Me7/em10400) linked to the RsDmR locus within a 10.0 cM distance were identified using bulked segregant analysis (BSA). The SRAP marker Em9/ga24370 was the most tightly linked one with a distance of 2.3 cM to RsDmR. These markers tightly linked to the RsDmR locus would facilitate marker-assisted selection and resistance gene pyramiding in radish breeding programs.
|
|
Received: 11 November 2013
Accepted:
|
| Fund: This work was in part supported by grants from the National Key Technologies R&D Program of China (2012BAD02B01), the Key Technology R&D Program of Jiangsu Province, China (BE2013429), Jiangsu Agricultural Science and Technology Innovation Fund (JASTIF,CX(12) 2006, (13)2007], NAUNKL- ZW2009007 and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). |
|
Corresponding Authors:
LIU Li-wang, Tel: +86-25-84395563, Fax: +86-25-84395266, E-mail: nauliulw@njau.edu.cn
E-mail: nauliulw@njau.edu.cn
|
| About author: XU Liang, E-mail: nauxuliang@njau.edu.cn,* These authors contributed equally to this study. |
Cite this article:
XU Liang, JIANG Qiu-wei, WU Jian, WANG Yan, GONG Yi-qin, WANG Xian-li, Limera Cecilia , LIU Li-wang.
2014.
Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.). Journal of Integrative Agriculture, 13(11): 2362-2369.
|
| Admassu B, Perovic D, Friedt W, Ordon F. 2011. Geneticmapping of the stem rust (Puccinia graminis f. sp. triticiEriks. & E. Henn) resistance gene Sr13 in wheat (Triticumaestivum L.). Theoretical and Applied Genetics, 122,643-648Allen R L, Bittner-Eddy P D, Grenville-Briggs L J, Meitz J C,Rehmany A P, Rose L E, Beynon J L. 2004. Host-parasitecoevolutionary conflict between Arabidopsis and downymildew. Science, 306, 1957-1960Boer E D, Zhang N W, Pelgrom K, Visser R G F, Niks R E,Jeuken M J W. 2013. Fine mapping quantitative resistancesto downy mildew in lettuce revealed multiple sub-QTLswith plant stage dependent effects reducing or evenpromoting the infection. Theoretical and Applied Genetics,126, 2995-3007Coelho P S, Monteiro A A. 2003. Expression of resistanceto downy mildew at cotyledon and adult plant stages inBrassica oleracea L. Euphytica, 133, 279-284Constantinescu O, Fatehi J. 2002. Peronospora-like fungi(Chromista peronosporales) parasitic on Brassicaceae andrelated hosts. Nova Hedwigia, 74, 291-334Farinhó M, Coelho P, Carlier J, Svetleva D, Monteiro A, LeitaoJ. 2004. Mapping of a locus for adult plant resistance todowny mildew in broccoli (Brassica oleracea convar.italica). Theoretical and Applied Genetics, 109, 1392-1398Farnham M, Wang M, Thomas C E. 2002. A single dominantgene for downy mildew resistance in broccoli. Euphytica,128, 405-407Franchel J, Bouzidi M F, Bronner G, Vear F, Nicolas P,Mouzeyar S. 2013. Positional cloning of a candidate genefor resistance to the sunflower downy mildew, Plasmoparahalstedii race 300. Theoretical and Applied Genetics, 126,359-367Gaspero G D, Copetti D, Coleman C, Castellarin S D, EibachR, Kozma P, Lacombe T, Gambetta G, Zvyagin A, Cindri?P, Kovács L, Morgante M, Testolin R. 2012. Selectivesweep at the Rpv3 locus during grapevine breedingfor downy mildew resistance. Theoretical and AppliedGenetics, 124, 277-286Jiang L N, Wang L J, Liu L W, Zhu X W, Zhai L L, Gong Y Q.2012. Development and characterization of cDNA librarybased novel EST-SSR marker in radish (Raphanus sativusL.). Scientia Horticulturae, 140, 164-172Jiang M, He C M, Miao L X, Zhang Y C. 2012. Overexpressionof a broccoli defensin gene BoDFN enhances downymildew resistance. Journal of Integrative Agriculture,11, 1137-1144Katula-Debreceni D, Lencsés A K, Sz?ke A, Veres A,Hoffmann S, Kozma P, Kovács L G, Heszky L, Kiss E.2010. Marker-assisted selection for two dominant powderymildew resistance genes introgressed into a hybrid grapepopulation. Scientia Horticulturae, 126, 448-453Kim S, Song Y H, Lee J Y, Choi S R, Dhandapani V, Jang CS, Lim Y P, Han T. 2011. Identification of the BrRHP1locus that confers resistance to downy mildew in Chinesecabbage (Brassica rapa ssp. pekinensis) and developmentof linked molecular markers. Theoretical and AppliedGenetics, 123, 1183-1192Kosambi D D. 1944. The estimation of map distances fromrecombination values. Annals of Eugenics, 12, 172-175Li G, Quiros C F. 2001. Sequence-related amplifiedpolymorphism (SRAP), a new marker system based on asimple PCR reaction: its application to mapping and genetagging in Brassica. Theoretical and Applied Genetics,107, 168-180Liu L W, Guo W Z, Zhu X F, Zhang T Z. 2003. Inheritanceand fine mapping of fertility-restoration for cytoplasmicmale sterility in Gossypium hirsutum L. Theoretical andApplied Genetics, 106, 461-469Liu L W, Zhao L P, Gong Y Q, Wang M X, Chen L M, Yang JL, Wang Y, Yu F M, Wang L Z. 2008. DNA fingerprintingand genetic diversity analysis of late-bolting radishcultivar with RAPD, ISSR and SRAP markers. ScientiaHorticulturae, 116, 240-247Liu Z, Gulya T J, Seiler G J, Vick B A, Jan C C. 2012.Molecular mapping of the Pl16 downy mildew resistancegene from HA-R4 to facilitate marker-assisted selectionin sunflower. Theoretical and Applied Genetics, 125,121-131Michelmore R W, Paran I, Kesseli R V. 1991. Identificationof markers closely linked to disease-resistance genesby bulked segregant analysis: A rapid method to detectmarkers in specific genomic regions by using segregatingpopulations. Proceedings of the National Academy ofSciences of the United States of America, 88, 9828-9832Molinero-Ruiz M L, Melero-Vara J M, Dominguez J. 2003.Inheritance of resistance to two races of sunflower downymildew (Plasmopara halstedii) in two Helianthus annuusL. lines. Euphytica, 131, 47-51Monteiro A A, Coelho P S, Bahcevandziev K, Valério L. 2005.Inheritance of downy mildew resistance at cotyledon and adult-plant stages in ‘Couve Algarvia’ (Brassica oleraceavar. tronchuda). Euphytica, 141, 85-92Mulpuri S, Liu Z, Feng J H, Gulya T, Jan C C. 2009. Inheritanceand molecular mapping of a downy mildew resistancegene, Pl13 in cultivated sunflower (Helianthus annuus L.).Theoretical and Applied Genetics, 119, 795-803Mutlu N, Miklas P N, Coyne D P. 2006. Resistance gene analogpolymorphism (RGAP) markers co-localize with diseaseresistance genes and QTL in common bean. MolecularBreeding, 17, 127-135Nashaat N I, Heran A, Mitchell S E, Awasthi R P. 1997.New genes for resistance to downy mildew (Peronosporaparasitica) in oilseed rape (Brassica napus ssp. oleifera).Plant Pathology, 46, 964-968van Ooijen J W, Voorrips R E. 2001. JoinMap Version 3.0.Software for the calculation of genetic linkage maps. PlantResearch International, Wageningen.Perazzolli M, Moretto M, Fontana P, Ferrarini A, Velasco R,Moser C, Delledonne M, Pertot I. 2012. Downy mildewresistance induced by Trichoderma harzianum T39 insusceptible grapevines partially mimics transcriptionalchanges of resistant genotypes. BMC Genomics, 13, 660.Radwan O, Bouzidi M F, Mouzeyar S. 2011. Molecularcharacterization of two types of resistance in sunflower toPlasmopara halstedii, the causal agent of downy mildew.Phytopathology, 101, 970-979Riaz S, Tenscher A C, Ramming D W, Walker M A. 2011.Using a limited mapping strategy to identify major QTLsfor resistance to grapevine powdery mildew (Erysiphenecator) and their use in marker-assisted breeding.Theoretical and Applied Genetics, 122, 1059-1073Seehalak W, Moonsom S, Metheenukul P, Tantasawat P.2011. Isolation of resistance gene analogs from grapevineresistant and susceptible to downy mildew and anthracnose.Scientia Horticulturae, 128, 357-363Vear F, Serre F, Jouan-Dufournel I, Bert P F, Roche S,Walser P, de Tourvieille Labrouhe D, Vincourt P. 2008.Inheritance of quantitative resistance to downy mildew(Plasmopara halstedii) in sunflower (Helianthus annuusL.). Euphytica, 164, 561-570Voorrips R E. 2002. MapChart: Software for the graphicalpresentation of linkage maps and QTL. Journal ofHeredity, 93, 77-78Wang L Z, He Q W. 2005. Chinese Radish. Scientific andTechnical Documents Publishing House, Beijing. (inChinese)Wang P, Liu C Y, Wang D X, Liang C H, Zhao K H, Fan J J.2013. Isolation of resistance gene analogs from grapevineresistant to downy mildew. Scientia Horticulturae, 150,326-333Wen J, Can V T, Zhang Y M. 2013. Multi-QTL mappingfor quantitative traits using distorted markers. MolecularBreeding, 31, 395-404Xu L, Wang L J, Gong Y Q, Dai W H, Wang Y, Zhu X W,Wen T C, Liu L W. 2012. Genetic linkage map constructionand QTL mapping of cadmium accumulation in radish(Raphanus sativus L.). Theoretical and Applied Genetics,125, 659-670Xu S. 2008. Quantitative trait locus mapping can benefit fromsegregation distortion. Genetics, 180, 2201-2208Yu S C, Zhang F G, Yu R B, Zou Y M, Qi J N, Zhao X Y,Yu Y J, Zhang D S, Li L. 2009. Genetic mapping andlocalization of a major QTL for seedling resistance todowny mildew in Chinese cabbage (Brassica rapa ssp.pekinensis). Molecular Breeding, 23, 573-590Zhang L Y, Wang S Q, Li H H, Deng Q M, Zheng A P, LiS C, Li P, Li Z L, Wang J K. 2010. Effects of missingmarker and segregation distortion on QTL mapping inF2 populations. Theoretical and Applied Genetics, 121,1071-1082 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
