|
|
|
|
|
| Ethylene and Spermidine in Wheat Grains in Relation to Starch Content and Granule Size Distribution Under Water Deficit |
| YANG Wei-bing, LI Yong, YIN Yan-ping, JIANG Wen-wen, PENG Dian-liang, CUI Zheng-yong, YANG Dong-qing , WANG Zhen-lin |
| National Key Laboratory of Crop Biology, Ministry of Science and Technology/Agronomy College, Shandong Agricultural University, Tai’an 271018, P.R.China |
|
|
|
摘要 Two wheat cultivars (Triticum aestivum L.) were used to evaluate the effects of post-anthesis severe water deficit (SD) on starch content and granule size distribution and their relations with ethylene and spermidine (Spd). Comparison to the well-watered (WW) treatment, SD led to lower Spd and higher 1-aminocylopropane-1-carboxylic acid (ACC) concentrations and ethylene evolution rate (EER) in grains at the critical stage of forming starch granules. Application of Spd or aminoethoxyvinylglycine (AVG) significantly reduced ACC concentration and EER and increased Spd concentration, while ethephon or methylglyoxal-bis (MGBG) had an opposite impact. The volume and surface area distribution of starch granules showed a bimodal curve, while the number distribution exhibited a unimodal curve. SD caused a marked drop in grain weight, grain number and starch content, also led to a significant reduction in the proportion (both by volume and by surface area) of B-type starch granules (<10 μm), with an increase in those of A-type starch granules (>10 μm). Application of Spd or AVG increased the proportion (both by volume and by surface area) of B-type starch granules under SD. Correlation analysis suggested that ethylene and Spd showed an antagonism relation in the formation of B-type granules. These results suggested that it would be good for the formation of B-type starch granules to have the physiological traits of higher Spd and lower ACC concentrations and ethylene emission under SD.
Abstract Two wheat cultivars (Triticum aestivum L.) were used to evaluate the effects of post-anthesis severe water deficit (SD) on starch content and granule size distribution and their relations with ethylene and spermidine (Spd). Comparison to the well-watered (WW) treatment, SD led to lower Spd and higher 1-aminocylopropane-1-carboxylic acid (ACC) concentrations and ethylene evolution rate (EER) in grains at the critical stage of forming starch granules. Application of Spd or aminoethoxyvinylglycine (AVG) significantly reduced ACC concentration and EER and increased Spd concentration, while ethephon or methylglyoxal-bis (MGBG) had an opposite impact. The volume and surface area distribution of starch granules showed a bimodal curve, while the number distribution exhibited a unimodal curve. SD caused a marked drop in grain weight, grain number and starch content, also led to a significant reduction in the proportion (both by volume and by surface area) of B-type starch granules (<10 μm), with an increase in those of A-type starch granules (>10 μm). Application of Spd or AVG increased the proportion (both by volume and by surface area) of B-type starch granules under SD. Correlation analysis suggested that ethylene and Spd showed an antagonism relation in the formation of B-type granules. These results suggested that it would be good for the formation of B-type starch granules to have the physiological traits of higher Spd and lower ACC concentrations and ethylene emission under SD.
|
|
Received: 07 August 2013
Accepted:
|
| Fund: We gratefully acknowledge the support of the National Natural Science Foundation of China (31271661, 30871477), the National Basic Research Program of China (2009CB118602), the Special Fund for Agro-Scientific Research in the Public Interest of China (201203100), and the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2012BAD04B05). |
|
Corresponding Authors:
WANG Zhen-lin, Tel/Fax: +86-538-8241359, E-mail: Zlwang@sdau.edu.cn
E-mail: Zlwang@sdau.edu.cn
|
| About author: YANG Wei-bing, E-mail: shennong05@126.com;These authors contributed equally to this study. |
Cite this article:
YANG Wei-bing, LI Yong, YIN Yan-ping, JIANG Wen-wen, PENG Dian-liang, CUI Zheng-yong, YANG Dong-qing , WANG Zhen-lin.
2014.
Ethylene and Spermidine in Wheat Grains in Relation to Starch Content and Granule Size Distribution Under Water Deficit. Journal of Integrative Agriculture, 13(10): 2141-2153.
|
| Ahmadi A, Baker D A. 2001. The effect of water stress on theactivities of key regulatory enzymes of the sucrose to starchpathway in wheat. Plant Growth Regulation, 35, 81-91Alcazar R, Marco F, Cuevas J C, Patron M, Ferrando A,Carrasco P, Tiburcio A F, Altabella T. 2006. Involvementof polyamines in plant response to abiotic stress.Biotechnology Letters, 28, 1867-1876Bechtel D B, Wilson J D. 2003. Amyloplast formation andstarch granule development in hard red winter wheat.Cereal Chemistry, 80, 175-183Bechtel D B, Zayas I Y, Kaleikau L, Pomeranz Y. 1990. Sizedistribution of wheat starch granules during endospermdevelopment. Cereal Chemistry, 67, 59-63Beltrano J, Ronco M G, Montaldi E R. 1999. Drought stresssyndrome in wheat is provoked ethylene evolution imbalanceand reversed by rewatering, aminoethoxyvinylglycine, orsodium benzoate. Journal of Plant Growth Regulation,18, 59-64Blumenthal C, Bekes F, Gras P W, Barlow E W, Wrigley CW. 1995. Identification of wheat genotypes tolerant to theeffects of heat stress on grain quality. Cereal Chemistry,72, 539-544Capell T, Bassie L, Christou P. 2004. Modulation of thepolyamine biosynthetic pathway in transgenic rice conferstolerance to drought stress. Proceedings of the NationalAcademy of Sciences of the United States of America, 101,9909-9914Cheng CY, Lur H S. 1996. Ethylene may be involved inabortion of the maize caryopsis. Physiology Plant, 98,245-252Dai Z M, Yin Y P, Zhang M, Li W Y, Yan S H, Cai R G, WangZ L. 2008. Starch granule size distribution in wheat grainsunder irrigated and rainfed conditions. Acta AgronomicaSinica, 34, 795-802 (in Chinese)Davies P J. 1995. Introduction. In: Davies P J, ed., PlantHormones, Physiology, Biochemistry and MolecularBiology. Kluwer Academic Publishers, Dordrecht, theNetherlands. pp. 1-12Duan H G, Yuan S, Liu W J, Xi D H, Qing D H, liang HG, Lin H H. 2006. Effects of exogenous ssermidine onphotosystem II of wheat seedlings under water stress.Journal of Integrative Plant Biology, 48, 920-927Feng H Y, Wang Z M, Kong F N, Zhang M J, Zhou S L. 2011.Roles of carbohydrate supply and ethylene, polyaminesin maize kernel set. Journal of Integrative Plant Biology,53, 388-398Flores H E, Galston A W. 1982. Analysis of polyamines inhigher plants by high performance liquid chromatography.Plant Physiology, 69, 701-706He Z F. 1985. Analysis Technique for Grain Quality in Cerealsand Oils. Agriculture Press, Beijing, China. pp. 290-294 (in Chinese)Hurkman W J, McCue KF, Altenbach S B. 2003. Effect oftemperature on expression of genes encoding enzymes forstarch biosynthesis in developing wheat endosperm. PlantScience, 164, 873-881Jiang D Y, Yu Z W. 2007. Effects of soil water on yield andgrain quality of wheat. Journal of Agricultural Science andTechnology, 21, 641-645 (in Chinese)Li W Y, Yan S H, Yin Y P, Wang Z L. 2010. Starch granulesize distribution in wheat grain in relation to shading afteranthesis. Journal of Agricultural Science, 148, 183-189Liu J H, Kitashiba H, Wang J, Ban Y, Moriguchi T. 2007.Polyamines and their ability to provide environmentalstress tolerance to plants. Plant Biotechnology, 24, 117-126Macleod L C, Duffus C M. 1988. Temperature effects on starchgranules in developing barley grains. Journal of CerealScience, 8, 29-37Morgan P W, Drew M C. 1997. Ethylene and plant responseto stress. Physiologia Plantarum, 100, 620-630Naik P K, Mohapatra P K. 2000. Ethylene inhibitors enhancedsucrose synthase activity and promoted grain filling ofbasal rice kernels. Australian Journal of Plant Physiology,27, 997-1008Narayana I, Lalonde S, Saini H S. 1991. Water-stress-inducedethylene production in wheat, a factor artifacting. PlantPhysiology, 96, 406-410Ni Y L, Wang Z L, Yin Y P, Li WY, Yan S H, Cai T. 2011.Starch granule size distribution in wheat grain in relation tophosphorus fertilization. Journal of Agricultural Science,10, 1-8Panozzo J F, Eagles H A. 1998. Cultivar and environmentaleffects on quality characters in wheat I: Starch. AustralianJournal of Agriculture Research, 49, 757-766Papadakis K A, Roubelakis-Angelakis K A. 2005. Spatial andtemporal distribution of polyamine levels and polyamineanabolism in different organs/tissues of the tobaccoplant. Correlations with age, cell division/expansion, anddifferentiation. Plant Physiology, 220, 826-837Park S H, Chung O K, Seib P A. 2004. Size distribution andproperties of wheat starch granules in relation to crumbgrain score of pup-loaf bread. Cereal Chemistry, 81,699-704Parker M L. 1985. The relationship between A-type and B-typestarch granules in the developing endosperm of wheat.Journal of Cereal Science, 3, 271-278 Peng M S, Gao M. 1999. Separation and characterization ofA- and B-type starch granules in wheat endosperm. CerealChemistry, 76, 375-379Peterson D G, Fulcher R G. 2001. Variation in Minnesota HRSwheats: starch granule size distribution. Food ResearchInternational, 34, 357-363Raeker M O, Gaines C S, Finney P L, Donelson T. 1998.Granule size distribution and chemical composition ofstarches from 12 soft wheat cultivars. Cereal Chemistry,75, 721-728Soh H N, Sissons M J, Turner M A. 2006. Effect of starchgranule size distribution and elevated amylose contenton durum dough rheology and spaghetti cooking quality.Cereal Chemistry, 83, 513-519Tester R F, Karkalas J, Qi X. 2004. Starch-composition, finestructure and architecture. Journal of Cereal Science, 39,151-165Vincent T, Farhang R, Delennea J Y. 2008. Wheat endospermas a cohesive granular material. Journal of Cereal Science,47, 347-356Wang Z, Xu Y, Wang J, Yang J, Zhang J. 2012. Polyamine andethylene interactions in grain filling of superior and inferiorspikelets of rice. Plant Growth Regulation, 66, 215-228Wilson J D, Bechtel D, Todd T C, Seib P A. 2006.Measurement of wheat starch granule size distributionusing image analysis and laser diffraction technology.Cereal Chemistry, 83, 259-268Xu Z Z, Yu Z W, Zhang Y L. 2003. The effects of soil moistureon grain starch synthesis and accumulation of winter wheat.Acta Agronomica Sinica, 29, 595-600(in Chinese)Yang J C, Cao Y, Zhang J H, Liu L, Zhang J. 2008. Involvementof polyamines in the post-anthesis development of inferiorand superior spikelets in rice. Planta, 228, 137-149Yang J C, Zhang J H, Liu K, Wang Z Q, Liu L J. 2006. Abscisicacid and ethylene interact in wheat grains in response to soildrying during grain filling. New Phytologist, 171, 293-303Yang J C, Zhang J H, Ye Y X, Wang Z Q, Zhu Q S, Liu LJ. 2004. Involvement of abscisic acid and ethylene in theresponses of rice grains to water stress during filling. Plant,Cell and Environment, 27, 1055-1064Yang W B, Yin Y P, Li Y, Cai T, Ni Y L, Peng D L, WangZ L. 2014. Interactions between polyamines and ethyleneduring grain filling in wheat grown under water deficitconditions. Plant Growth Regulation, 72, 189-201Zadoks J C, Chang T T, Konzak C F. 1974. A decimal code forthe growth stages of cereals. Weed Research, 14, 415-421Zhang C H, Jiang D, Li F, Cai J, Dai T B, Cao W X. 2010.Starch granules size distribution in superior and inferiorgrains of wheat is related to enzyme activities and theirgene expressions during grain filling. Journal of CerealScience, 51, 226-233Zhang T B, Wang Z L, Yin Y P, Cai R G, Yan S H, Li W Y.2010. Starch content and granule size distribution in grainsof wheat in relation to post-anthesis water deficits. Journalof Agronomy and Crop Science, 196, 1-8Zhao H, Dai T, Jiang D, Cao W X. 2008. Effects of hightemperature on key enzymes involved in starch and proteinformation in grains of two wheat cultivars. Journal ofAgronomy and Crop Science, 194, 47-54. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
