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| Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach |
| ZHAO Xiang-xiang, TANG Tang, LIU Fu-xia, LU Chang-li, HU Xiao-lan, JI Li-lian , LIU Qiaoquan |
1.Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian 223300, P.R.China
2.Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics, Ministry of Education/Yangzhou University, Yangzhou 225009, P.R.China |
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摘要 Development of new technologies for evaluating genetically modified (GM) crops has revealed that there are unintended insertions and expression changes in GM crops. Profiling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the application of a comparative proteomic approach to investigate the protein profile differences between a GM rice line, which has a lysine-rich protein gene, and its non-transgenic parental line. Proteome analysis by two-dimensional gel electrophoresis (2-DE) and mass spectrum analysis of the seeds identified 22 differentially expressed protein spots. Apart from a number of glutelins that were detected as targeted proteins in the GM line, the majority of the other changed proteins were involved in carbohydrate metabolism, protein synthesis and stress responses. These results indicated that the altered proteins were not associated with plant allergens or toxicity.
Abstract Development of new technologies for evaluating genetically modified (GM) crops has revealed that there are unintended insertions and expression changes in GM crops. Profiling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the application of a comparative proteomic approach to investigate the protein profile differences between a GM rice line, which has a lysine-rich protein gene, and its non-transgenic parental line. Proteome analysis by two-dimensional gel electrophoresis (2-DE) and mass spectrum analysis of the seeds identified 22 differentially expressed protein spots. Apart from a number of glutelins that were detected as targeted proteins in the GM line, the majority of the other changed proteins were involved in carbohydrate metabolism, protein synthesis and stress responses. These results indicated that the altered proteins were not associated with plant allergens or toxicity.
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Received: 14 June 2013
Accepted:
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| Fund: This research was supported by the National Major Special Project for the Development of Transgenic Organisms, China (2009ZX-08011-003B), the Jiangsu Province Qing Lan Project for Young and Middle-Aged Academic Leaders, China and the Jiangsu Province Qing Lan Project for Outstanding Scientific and Technological Innovation Team, China. |
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Corresponding Authors:
Correspondence JI Li-lian, Tel/Fax: +86-517-83525885, E-mail: jll2663@sina.com; LIU Qiao-quan, Tel/Fax: +86-514-87996648, E-mail: qqliu@yzu.edu.cn
E-mail: jll2663@sina.com
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| About author: ZHAO Xiang-xiang, E-mail: xxzhao2013@163.com |
Cite this article:
ZHAO Xiang-xiang, TANG Tang, LIU Fu-xia, LU Chang-li, HU Xiao-lan, JI Li-lian , LIU Qiaoquan.
2013.
Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach. Journal of Integrative Agriculture, 12(11): 2013-2021.
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