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| Selection of Submergence Tolerant Homozygous Line by STS Marker and Twice Submergence Stress |
| LI Jin-jiang, XIAO You-lun, XIAO Guo-ying |
| 1.Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Changsha 410125, P.R.China 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R.China 3.Hunan Plant Protection Institute, Changsha 410125, P.R.China 4.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P.R.China |
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摘要 One sequence tagged site marker Sub1-1 and twice submergence stress method were used in selection of submergence tolerant homozygous line from Sub-1BS, a submergence tolerant, bentazon sensitive and photoperiod-sensitive and/or thermo-sensitive genic male sterile line that developed by our laboratory. The results revealed that the original Sub-1BS was heterozygous in Sub1A-1 locus even though it was identical in almost all of agronomical traits and the segregation of Sub1A-1 was in accordance with Mendelian law based on chi-square test. And then the original Sub-1BS was divided into two groups: one was of Sub1A-1 introgression and the other was not; and the two groups were tested by twice submergence stress method. After the first submergence stress that lasted for 12 d, the average plant heights were significant difference at the 1% level between the two groups. After recovery for 10 d, the second submergence stress sustained for 18 d was carried on; and the group with Sub1A-1 gene was found apparently tolerant than the other group in submergence tolerance.
Abstract One sequence tagged site marker Sub1-1 and twice submergence stress method were used in selection of submergence tolerant homozygous line from Sub-1BS, a submergence tolerant, bentazon sensitive and photoperiod-sensitive and/or thermo-sensitive genic male sterile line that developed by our laboratory. The results revealed that the original Sub-1BS was heterozygous in Sub1A-1 locus even though it was identical in almost all of agronomical traits and the segregation of Sub1A-1 was in accordance with Mendelian law based on chi-square test. And then the original Sub-1BS was divided into two groups: one was of Sub1A-1 introgression and the other was not; and the two groups were tested by twice submergence stress method. After the first submergence stress that lasted for 12 d, the average plant heights were significant difference at the 1% level between the two groups. After recovery for 10 d, the second submergence stress sustained for 18 d was carried on; and the group with Sub1A-1 gene was found apparently tolerant than the other group in submergence tolerance.
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Received: 10 October 2011
Accepted:
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| Fund: This work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2- EW-N-01). |
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Corresponding Authors:
Correspondence XIAO Guo-ying, Tel: +86-731-84619770, Fax: +86-731-84612685, E-mail: xiaoguoying@isa.ac.cn
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Cite this article:
LI Jin-jiang, XIAO You-lun, XIAO Guo-ying.
2012.
Selection of Submergence Tolerant Homozygous Line by STS Marker and Twice Submergence Stress. Journal of Integrative Agriculture, 12(12): 1940-1947.
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