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| Molecular Cloning and Characterization of Carnation EBF1 Gene During Flower Senescence and upon Ethylene Exposure and Sugar |
| FU Zhao-di, WANG Hui-nan, LIU Juan-xu, ZENG Hong-xue, ZHANG Jiao, KUANG Xiao-cong , YUYi-xun |
1. College of Horticulture, South China Agricultural University, Guangzhou 510642, P.R.China
2. Zhejiang Tongji Vocational College of Science and Technology, Hangzhou 311231, P.R.China |
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摘要 A cDNA clone encoding a putative EBF-like protein (DCEBF1) was obtained from total RNA isolated from senescing carnation (Dianthus caryophyllus L.) petals using reverse transcription PCR and rapid-amplification of cDNA ends techniques. The cDNA contained an open reading frame of 1 878 bp corresponding to 625 amino acids. Results of Northern blot indicated DCEBF1 expression was enhanced by endogenous and exogenous ethylene, and was inhibited by STS in petals and ovaries. Upon wounding treatment, DCEBF1 showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The levels of DCEBF1 mRNA increased in both petals and ovaries by sucrose treatment compared with the control.
Abstract A cDNA clone encoding a putative EBF-like protein (DCEBF1) was obtained from total RNA isolated from senescing carnation (Dianthus caryophyllus L.) petals using reverse transcription PCR and rapid-amplification of cDNA ends techniques. The cDNA contained an open reading frame of 1 878 bp corresponding to 625 amino acids. Results of Northern blot indicated DCEBF1 expression was enhanced by endogenous and exogenous ethylene, and was inhibited by STS in petals and ovaries. Upon wounding treatment, DCEBF1 showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The levels of DCEBF1 mRNA increased in both petals and ovaries by sucrose treatment compared with the control.
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Received: 24 September 2010
Accepted:
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| Fund: This study was supported by the National Natural Science Foundation of China (30800758 and 30972410), SRF for ROCS, SEM, Fok Ying Tung Education Foundation (104031), China, and the Plant Biology National Experimental Teaching Demonstration Center of South China Agricultural University, China. |
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Corresponding Authors:
Correspondence YU Yi-xun, Tel: +86-20-85280228, Fax: +86-20-85282107, E-mail: yuyixun@scau.edu.cn
E-mail: yuyixun@scau.edu.cn
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Cite this article:
FU Zhao-di, WANG Hui-nan, LIU Juan-xu, ZENG Hong-xue, ZHANG Jiao, KUANG Xiao-cong , YUYi-xun .
2011.
Molecular Cloning and Characterization of Carnation EBF1 Gene During Flower Senescence and upon Ethylene Exposure and Sugar. Journal of Integrative Agriculture, 10(12): 1872-1880.
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